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Preparation method of eurotium cristatum spore suspension

A technology of spores of Saccharomyces coronoides and Saccharomyces coronoides, applied in the direction of fungi, etc., which can solve the problems of tea leaves not growing

Inactive Publication Date: 2011-07-13
HUNAN CITY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experimental data show that Fuzhuan brick tea hardly grows or does not grow well on the tea leaves at the raw material stage.

Method used

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  • Preparation method of eurotium cristatum spore suspension
  • Preparation method of eurotium cristatum spore suspension
  • Preparation method of eurotium cristatum spore suspension

Examples

Experimental program
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Effect test

preparation example Construction

[0033] 2. Preparation of liquid culture medium for Saccharomyces coronoides:

[0034] Preparation of tea juice: leaching in a water bath at 100°C for 2 hours at a ratio of 1:30 (mass ratio) to tea and water, and filtering through double-layer gauze for later use.

[0035] Potato juice preparation: leaching in a water bath at 100° C. for 1 hour at a ratio of potato to water of 3:10 (mass ratio), filtering with double-layer gauze for later use.

[0036] Accurately weigh 200 grams of sucrose and 100 grams of NaCl and dissolve them in appropriate amount of distilled water successively, add 100 mL of prepared tea juice and 300 mL of prepared potato juice, and finally set the volume to 1000 mL.

[0037] Divide into 250mL shaker flasks, add about 20 glass beads, and sterilize at 121°C for 20 minutes.

[0038] 3. Cultivation of the spores of S. coronoides:

[0039] The culture condition is 28°C, static culture for 5-7 days; the later temperature is 30-32°C, static culture for 2-3 da...

Embodiment 1

[0050] Embodiment 1: Separation and purification of Flos Chinensis:

[0051] The finished sample of Xiangyi brand Fuzhuan brick tea was pulverized with a plant micro pulverizer, sieved with 200 mesh, dried at 65°C for 2 hours, weighed 1g of the sample, fixed to a volume of 10mL, and prepared a concentration of 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Each 10mL, take 10 -3 、10 -4 、10 -5 Spread 0.0.5ml of each of the three gradients on a PDA plate added with 10% tea juice, blow dry on an ultra-clean bench, incubate at 28°C for 3 days, pick golden yellow dominant colonies, and draw a line on the PDA plate added with 10% tea juice On the same condition continue to culture until purification.

[0052] The isolated and purified golden flower colonies are shown in the appendix figure 1 .

Embodiment 2

[0053] Embodiment 2: the spore suspension preparation of golden flower:

[0054]Pick the purified golden flower colony and inoculate it into the PDA liquid medium with 10% tea juice, 20% sucrose, and 10% NaCl, the culture condition is 28°C, and the culture is static for 5-7 days; -32°C, static culture for 2-3 days, then 280rpm shaking culture to disperse conidia, filter with double-layer sterile gauze, and measure the OD value of 0.6-0.8 with a 722-type spectrophotometer, which is the available gold. flower spore suspension.

[0055] The results of the spore suspension culture of Jinhua are attached figure 2 , attached image 3 , attached Figure 4 .

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Abstract

The invention discloses a preparation method of eurotium cristatum spore suspension. The preparation method is characterized by comprising the following steps of: 1) culturing eurotium cristatum; 2) preparing a liquid culture medium of the eurotium cristatum; 3) culturing eurotium cristatum spores; 4) dispersing the eurotium cristatum spores; 5) filtering the eurotium cristatum spores; 6) determining the concentration of the eurotium cristatum spores; and 7) storing the eurotium cristatum spores. The eurotium cristatum spore suspension has high concentration, the eurotium cristatum spores have uniform and hypertrophic size and bright color, and the eurotium cristatum spore suspension can be prepared within a short period of 10 days and can full meet the requirement of eurotium cristatum inoculation of Fuzhuan tea, and has stable and reliable quality.

Description

Technical field: [0001] The invention relates to the technical field of agricultural product processing, and in particular to the culture conditions and preparation method of the spore suspension of the dominant fungus of Fuzhuan tea, S. coronis, commonly known as Jinhua. Background technique [0002] For a long time, Fuzhuan brick tea has been an indispensable product in the daily life of ethnic minorities in the frontier. It enjoys the reputation of "it is better to be without food for three days than to be without tea for a day" in the frontier. Its mystery is inseparable from the quality and flavor of Fuzhuan brick tea. divided. Blooming is the key process to form the unique quality and flavor of Fuzhuan tea. Its purpose is to promote the growth and reproduction of the dominant bacteria in Fuzhuan tea - Saccharomyces coronata through the control of external conditions (such as temperature, humidity, etc.), so that Fuzhuan Brick tea has a unique quality flavor. The fung...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 赵运林刘石泉
Owner HUNAN CITY UNIV
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