Brevibacillus brevis TW effervescent granule and preparation method thereof
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A technology of Bacillus microbacterium and effervescent granules, which is applied in the directions of botanical equipment and methods, fungicides, biocides, etc., can solve the problems of short validity period, difficult preservation, and inability to reach the diseased site, and achieves low cost and convenient application. Effect
Active Publication Date: 2011-07-20
内蒙古永太化学有限公司
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Problems solved by technology
At present, the control of rice sheath blight is mainly based on the agricultural antibiotic Jinggangmycin, but Jinggangmycin has been widely used since the 1970s, and the drug resistance has increased, and the dosage has gradually increased. Once the sheath blight breaks out, it is difficult to use Jinggangmycin. Controlling the damage of sheath blight in a short period of time
Especially at present, farmers use the method of low-water fine spraying, and the drug-receiving parts are mainly the leaves and
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Embodiment 1
[0017] The preparation of embodiment 1 Bacillus pumilus TW effervescent granule
[0018] (1) Acquisition of biocontrol living bacteria liquid
[0019] Use a sterile bud stick to pick a single colony of Microbacillus brevis TW strain that has been streaked and cultured on LB solid medium in advance, inoculate it in a 20ml Erlenmeyer flask containing 5ml of PDA liquid culture medium, and incubate at 30°C 1. Shaking culture at 200rpm for 16 hours, overnight, inoculate all 5ml of the culture solution obtained in the previous step into a 1000ml Erlenmeyer flask containing 400ml of PDA culture solution, and shake at 200rpm and 30°C for 16 hours. In a 30L fermenter (the model is Zhenjiang Dongfang GUS-30), the 400ml nutrient solution obtained was inoculated into a 20L fermentation nutrient solution. Starch 100g, sucrose 25g, yeast powder 25g, calcium carbonate 20g, MnSO 4 1.0g, the rest is water. The fermentation conditions were set as follows: dissolved oxygen 100%, pH 7.0-7.2, s...
Embodiment 2
[0022] Example 2 Field control test of Bacillus brevis TW effervescent preparation on rice sheath blight
[0023] The test location: Binhai County, Jiangsu Province.
[0024] Experimental field conditions: The rice variety is Wuyunjing 3, the transplanting method is machine transplanting of small seedlings, and the field management is carried out according to conventional methods.
[0025] Experimental treatment method: at the end of rice tillering stage, it was divided into three groups (the plot area of each group was 40m2) 2 ):
[0026] Test group: use 500g of the effervescent preparation of Bacillus pumilus TW per mu, mix it with 20kg of fine soil and spread evenly.
[0027] Control agent group: use 500ml of 5% Jinggangmycin water agent per mu to prevent and control, and the water consumption per mu is 50kg.
[0028] Blank control group: spray with water.
[0029] The experiment was repeated 4 times. After 30 days, the disease investigation was carried out, and samp...
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Abstract
The invention relates to a brevibacillus brevis TW effervescent granule. The brevibacillus brevis TW effervescent granule is prepared through the following steps of: mixing a brevibacillus brevis TW strain single colony plaque PDA (Potato Dextrose Agar) liquid culture solution with soybean meal, corn meal, starches, cane sugar, yeast powder, calcium carbonate and MnSO4 by adding water; mixing bacterial fermentation liquor obtained by fermenting under the conditions of 100% dissolved oxygen, the pH of 7.0-7.2 and the temperature of 30 DEG C with the cane sugar, polyvinyl alcohol, citric acid monohydrate, potassium tartrate and sodium bicarbonate according to a proportion; screening, granulating, and drying through steam flows at a temperature of 60 DEG C, wherein the total concentration ofviable bacteria of a finished product is about 1*10<9>-1*10<10> cfu/g. The brevibacillus brevis TW effervescent granule can be effectively suspended on a water surface and then fast disintegrated, releases the biocontrol bacteria of living bodies and enables the biocontrol bacteria to attach to the contact surface of the stem of a rice plant and the water surface, thereby effectively cutting off or spatially occupying the initial invasion of rice rhizoctonia solani on the rice stem and achieving the purpose that control effect on rice sheath blight reaches more than 80 percent; and the brevibacillus brevis TW effervescent granule is convenient for application, has low cost and can be preserved for 2-3 years at normal temperature.
Description
technical field [0001] The invention relates to a small Bacillus pumilus TW effervescent granule preparation and a preparation method for preventing and treating rice sheath blight. Background technique [0002] Rice sheath blight (Rice sheathblight) caused by the plant pathogenic fungus Rhizoctonia solani (Rhizoctonia solani) is currently the main disease of rice in my country. The disease can cause 5% to 45% yield loss all year round, and 70% yield reduction in high-incidence years, or even no harvest. At present, the control of rice sheath blight is mainly based on the agricultural antibiotic Jinggangmycin, but Jinggangmycin has been widely used since the 1970s, and the drug resistance has increased, and the dosage has gradually increased. Once the sheath blight breaks out, it is difficult to use Jinggangmycin. Control the damage of sheath blight in the short term. Especially at present, farmers mostly use the method of low-water fine spraying, and the drug-receiving pa...
Claims
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