Method for analyzing algae and chormophoric dissolved organic matters on line by non-external sheath fluid flow cytometry

A liquid flow and cytometry technology, applied in the direction of individual particle analysis, particle and sedimentation analysis, measurement devices, etc., can solve the problems of large size, inability to realize online detection of colored soluble organics, high price, etc., to avoid biological pollution, The effect of preventing cyanobacterial blooms and improving accuracy

Inactive Publication Date: 2011-07-20
无锡荣兴科技有限公司
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At present, this test can only be done on a fluorescence spectrophotometer. The professional equipment used in the test is not only expensive, bu...

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  • Method for analyzing algae and chormophoric dissolved organic matters on line by non-external sheath fluid flow cytometry
  • Method for analyzing algae and chormophoric dissolved organic matters on line by non-external sheath fluid flow cytometry
  • Method for analyzing algae and chormophoric dissolved organic matters on line by non-external sheath fluid flow cytometry

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Embodiment Construction

[0037] The present invention will be further described below in conjunction with specific drawings and embodiments. The following embodiments are only used to further illustrate the present invention, and should not be construed as any limitation to the description.

[0038] The invention adopts flow cytometry to continuously detect algae and CDOM on-line. The algae in the detection object contain phycobiliprotein, and its main function is to serve as a light-harvesting pigment complex for photosynthesis, which is formed by the covalent combination of the pigment group phycobilin and the carrier protein. It is divided into four types: phycoerythrin, phycocyanin, phycoerythrin and allophycocyanin. Due to their different contents in different algae, some algae appear red, while others are green. The protein contained in domestic cyanobacteria is mainly phycocyanin. The maximum absorption peak of this protein is around 620nm, which can emit strong fluorescence, has good light abs...

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Abstract

The invention relates to a method for analyzing algae and chormophoric dissolved organic matters (CDOM) on line by non-external sheath fluid flow cytometry, which comprises the following steps of: collecting and treating samples, detecting the algae, filtering the algae, detecting CDOM and outputting a result and calculating. In the method, sheath fluid is non-external sheath fluid, namely after the samples are subjected to primary treatment and filtered to remove the algae, the sheath fluid can be used directly, so the trouble of replacing the sheath fluid is saved and the biological pollution of a liquid fluid system in a system is prevented. By the method, the algae and the chormophoric dissolved organic matters (CDOM) in water can be detected and analyzed on line simultaneously, and an early warning can be given for the eutrophic degree of water quality so as to prevent the breakout of cyanobacterial blooms. No matter a cyclic sheath fluid mode or an acyclic sheath fluid mode is adopted in the flow cytometry, the CDOM can be detected effectively, and the accuracy of detection results of the algae is improved; and more water quality information can be acquired under the condition of increasing limited use cost.

Description

technical field [0001] The invention belongs to the technical field of online detection and analysis of water quality components, and relates to the detection of algae and colored soluble organic matter (Chromophoric dissolved organic matter, CDOM) in water. Soluble organic matter method. Background technique [0002] Algae refers to a class of eukaryotes in the Protist kingdom (some are also prokaryotes, such as algae in the cyanobacteria). Mainly aquatic, without vascular bundles, capable of photosynthesis. It contains phycobiliprotein, whose main function is to serve as a light-harvesting pigment complex for photosynthesis, which is formed by covalently combining the pigment group phycobililin and the carrier protein. It is divided into four types: phycoerythrin, phycocyanin, phycoerythrin and allophycocyanin. Due to their different contents in different algae, some algae appear red, while others are green. The protein contained in domestic cyanobacteria is mainly phyc...

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Application Information

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IPC IPC(8): G01N15/14
Inventor 邵建辉楚建军赵丙强陈力王欢
Owner 无锡荣兴科技有限公司
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