Preparation method of asterias amurensis polysaccharide extractive and medical applications thereof
A technology for extracting polysaccharides from the spiny sea of thorns is applied in the research and development field of pharmaceutical products, and can solve the problems of less research on polysaccharides.
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Embodiment 1
[0014] Step (1): remove the viscera from the sea spinach disc, dry the body wall, crush through a 60-mesh sieve, and percolate 1000g of the powder with 4000mL of ethanol in a percolation device to obtain a filter residue;
[0015] Step (2): add the filter residue in step (1) to 5000mL 0.1mol / L NaOH solution, stir overnight at room temperature, and centrifuge to obtain a supernatant;
[0016] Step (3): adjust the pH of the supernatant in step (2) to 7.0, add 1% Bacillus subtilis neutral protease to hydrolyze overnight, and centrifuge to obtain the supernatant;
[0017] Step (4): adjust the pH of the supernatant in step (3) to 8.0, add 5% hydrogen peroxide, decolorize to light yellow at 50°C, concentrate under reduced pressure to obtain a concentrate;
[0018] Step (5): deproteinize the concentrated solution in step (4) with Sevage reagent twice, then add 5 times the amount of absolute ethanol, and centrifuge to obtain a precipitate;
[0019] Step (6): drying the precipitate in...
Embodiment 2
[0022] Step (1): remove the viscera from the sea spinach disc, dry the body wall, crush through a 60-mesh sieve, and percolate 800 g of the powder with 3200 mL of ethanol in a percolation device to obtain a filter residue;
[0023] Step (2): add the filter residue in step (1) to 4000 mL of 0.1mol / L NaOH solution, stir overnight at room temperature, and centrifuge to obtain a supernatant;
[0024] Step (3): adjust the pH of the supernatant in step (2) to 7.0, add 1% Bacillus subtilis neutral protease to hydrolyze overnight, and centrifuge to obtain the supernatant;
[0025] Step (4): adjust the pH of the supernatant in step (3) to 8.0, add 5% hydrogen peroxide, decolorize to light yellow at 50°C, concentrate under reduced pressure to obtain a concentrate;
[0026] Step (5): deproteinize the concentrated solution in step (4) with Sevage reagent twice, then add 4 times the amount of absolute ethanol, and centrifuge to obtain a precipitate;
[0027] Step (6): drying the precipita...
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