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Preparation method of asterias amurensis polysaccharide extract and medical applications thereof

A technology for extracting polysaccharides from the spiny sea of ​​thorns is applied in the research and development field of pharmaceutical products, and can solve the problems of less research on polysaccharides.

Inactive Publication Date: 2012-12-26
SHANDONG UNIV AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Active substances such as saponins and sterols in starfish have been deeply studied, but there are few studies on polysaccharides in starfish

Method used

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  • Preparation method of asterias amurensis polysaccharide extract and medical applications thereof

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Experimental program
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Effect test

Embodiment 1

[0014] Step (1): get rid of the internal organs of the spiny sea, the body wall is dried, pulverized and passed through a 60-mesh sieve, and 1000g of powder is percolated with 4000mL of ethanol in a percolation device to obtain a filter residue;

[0015] Step (2): add the filter residue in step (1) into 5000mL 0.1mol / L NaOH solution, stir at room temperature overnight, and centrifuge to obtain a supernatant;

[0016] Step (3): adjust the pH of the supernatant in step (2) to 7.0, add 1% Bacillus subtilis neutral protease for enzymatic hydrolysis overnight, and centrifuge to obtain the supernatant;

[0017] Step (4): adjust the pH of the supernatant in step (3) to 8.0, add 5% hydrogen peroxide, decolorize to light yellow at 50 ° C, and concentrate under reduced pressure to obtain a concentrated solution;

[0018] Step (5): deproteinize the concentrated solution in step (4) with Sevage reagent twice, then add 5 times the amount of absolute ethanol, and centrifuge to obtain precip...

Embodiment 2

[0022] Step (1): get rid of the internal organs of the spiny sea, the body wall is dried, pulverized and passed through a 60-mesh sieve, and 800g of powder is percolated with 3200mL of ethanol in a percolation device to obtain a filter residue;

[0023] Step (2): add the filter residue in step (1) into 4000mL 0.1mol / L NaOH solution, stir at room temperature overnight, and centrifuge to obtain a supernatant;

[0024] Step (3): adjust the pH of the supernatant in step (2) to 7.0, add 1% Bacillus subtilis neutral protease for enzymatic hydrolysis overnight, and centrifuge to obtain the supernatant;

[0025] Step (4): adjust the pH of the supernatant in step (3) to 8.0, add 5% hydrogen peroxide, decolorize to light yellow at 50 ° C, and concentrate under reduced pressure to obtain a concentrated solution;

[0026] Step (5): deproteinize the concentrated solution in step (4) with Sevage reagent twice, then add 4 times the amount of absolute ethanol, centrifuge to obtain precipitati...

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Abstract

The invention belongs to the technical field of medical product research and development and relates to a preparation method of an asterias amurensis polysaccharide extractive and medical application thereof. The preparation method comprises the following steps of: taking asterias amurensis, removing abdominal organs, drying the organ walls, crushing and filtering with a sieve of 60 meshes; seeping the powder in a seeping device with ethanol, adding 0.1 mol / L of NaOH solution to filter residue, stirring at a room temperature and standing overnight, centrifugating, adjusting the pH value of a supernatant to 7.0, adding 1% grass bacillus neutral proteinase for enzymolysis and standing overnight, centrifugating and adjusting the pH value to 8.0; adding 5% hydrogen peroxide, decoloring at 50 DEG C to be lightyellow, concentrating under a pressure reduction state and deproteinizing the concentrated liquid with a Sevage reagent twice; and adding absolute ethyl alcohol, centrifugating, precipitating, drying under a pressure reduction state and crushing to obtain asterias amurensis polysaccharide extractive powder. The product provided by the invention can improve the GSH (Glutathione) content in the liver, improve the detoxification capability of the liver, has the liver protection action and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of research and development of pharmaceutical products. The present invention relates to a preparation method and medical use of polysaccharide polysaccharide extracts from P. chinensis. The product of the invention can increase the content of glutathione (GSH) in the liver, improve the detoxification ability of the liver, and has the effect of protecting the liver, and the product of the invention is suitable for industrial production. Background technique [0002] Asterias amurensis belongs to the phylum Echinodermata, Asteroidea, Forcipulata, and Asteriidae. It is the main species in the northern waters of my country. Stomach, pain relief, analgesia and other effects. In recent years, Weihai has been flooded with starfish (mainly in the sea of ​​thorns), which seriously endangers shellfish farming. Therefore, the development and utilization of starfish resources has huge economic and social benefits. Ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/56A61P1/16A61K35/616
Inventor 张崇禧刘铁铮郭爱洁王韶莉王桂春吴泽华褚福龙
Owner SHANDONG UNIV AT WEIHAI
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