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Asterias amurensis enzymatic hydrolysis polypeptide and application thereof as ACE inhibitor

A technology of enzymolysis and enzymatic hydrolysis of sea thorns, which is applied in the field of enzymatic hydrolysis of peptides of sea thorns, can solve problems such as side effects, and achieve the effects of low cost, easy acquisition, and broad market prospects

Inactive Publication Date: 2016-07-20
DALIAN OCEAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use of current artificially synthesized antihypertensive drugs (such as captopril) often produces side effects of varying degrees, such as cardiovascular disease, angioedema, cough, rash, etc.

Method used

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  • Asterias amurensis enzymatic hydrolysis polypeptide and application thereof as ACE inhibitor
  • Asterias amurensis enzymatic hydrolysis polypeptide and application thereof as ACE inhibitor
  • Asterias amurensis enzymatic hydrolysis polypeptide and application thereof as ACE inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Put the whole thorny sea bark into a drying oven, dry and crush it at 65°C, pass through a 20-mesh sieve, mix the thorny sea bark powder with distilled water at a solid-to-liquid ratio of 1:20g / ml, And add pepsin to it, the added pepsin is 5% of the mass of sea thorn carcass, after stirring evenly, adjust the pH=2.0 with concentrated hydrochloric acid, and then enzymolyze it in a constant temperature water bath for 8 hours under the condition of 37°C , to obtain the enzymatic hydrolyzate of S. spinosae, put the enzymatic solution of S. spinacha in a boiling water bath for 10 minutes, and then centrifuge after cooling. Centrifugation conditions: 4000r / min, centrifuge for 15min, then take the supernatant, dialyze, concentrate, Freeze-dry to obtain pepsin-enzymatic polypeptide (PE-P).

Embodiment 2

[0024] Put the whole thorny sea bark into a drying oven, dry and crush it at 65°C, pass through a 20-mesh sieve, mix the thorny sea bark powder with distilled water at a solid-to-liquid ratio of 1:20g / ml, And add papain to it, the added papain is 5% of the mass of spiny caravan, after stirring evenly, adjust the pH=6.5 with concentrated hydrochloric acid, and then enzymolyze it in a constant temperature water bath for 8 hours under the condition of 60°C , to obtain the enzymatic hydrolyzate of S. spinosae, put the enzymatic solution of S. spinacha in a boiling water bath for 10 minutes, and then centrifuge after cooling. Centrifugation conditions: 4000r / min, centrifuge for 15min, then take the supernatant, dialyze, concentrate, Freeze-dry to obtain papain hydrolyzed polypeptide (PA-P).

Embodiment 3

[0026] Put the whole thorny sea bark into a drying oven, dry and crush it at 65°C, pass through a 20-mesh sieve, mix the thorny sea bark powder with distilled water at a solid-to-liquid ratio of 1:20g / ml, And add alkaline protease to it, the added alkaline protease is 5% of the quality of sea spiny caravan, after stirring evenly, adjust pH=12.0 with sodium hydroxide, and then under the condition of 50 ℃, enzyme in a constant temperature water bath Disintegrate for 8 hours to obtain the enzymatic hydrolyzate of sea spinach, put the enzymatic hydrolyzate of sea spinach in a boiling water bath for 10 minutes, and then centrifuge after cooling. Centrifugation conditions: 4000r / min, centrifuge for 15 minutes, then take the supernatant and dialyze , concentrated, and freeze-dried to obtain alkaline protease hydrolyzed polypeptide (AP-P).

[0027] The application of the above-mentioned three kinds of sea spinach enzymatically hydrolyzed polypeptides as ACE inhibitors.

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Abstract

The invention discloses asterias amurensis enzymatic hydrolysis polypeptide, wherein the asterias amurensis enzymatic hydrolysis polypeptide is prepared by the following steps: drying entire asterias amurensis in a drying oven at 65 DEG C and crushing the dried asterias amurensis; filtering the crushed asterias amurensis by virtue of a 20-mesh sieve; mixing the asterias amurensis powder with distilled water at the ratio of 1g to 20ml of solid to liquid; adding protease to an obtained mixture, wherein the dosage of the added protease is 5%of the mass of the asterias amurensis; uniformly stirring; enzymatically hydrolyzing in a thermostat water bath for 8h, so that asterias amurensis enzymatic hydrolysate is obtained; keeping the asterias amurensis enzymatic hydrolysate in a boiling water bath for 10min, cooling and conducting centrifugal treatment at 4000r / min for 15min; and then, reserving a supernatant liquid, dialyzing, concentrating and freeze-drying, so that the asterias amurensis enzymatic hydrolysis polypeptide is obtained. The invention also discloses application of the asterias amurensis enzymatic hydrolysis polypeptide as an ACE inhibitor.

Description

technical field [0001] The present invention relates to an enzymolyzed polypeptide of sea thorns and the specific application of the enzymatically hydrolyzed polypeptide of sea thorny as an ACE inhibitor. Background technique [0002] Asteria samurensis, in the shape of a five-pointed star with a diameter of about 14cm, a flat body, yellow or light brown, a slightly raised back, and a flat mouth, is a very common starfish along the coast of the Yellow Sea in my country. It belongs to the marine invertebrates, echinodermata, starfish class, and sea starfish family, and is a known edible starfish. Sea thorns are widely distributed in the Bohai Sea and the Yellow Sea in my country, and many kinds of steroidal saponins, sterols, polysaccharides, ceramides, and cerebrosides have been extracted from sea thorns. The thorny sea plate has a long history of consumption among the coastal residents of the Yellow Sea and the Bohai Sea in China. Its edible part is the gonad. The composit...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K38/01A61P9/12A23L33/18
CPCC12P21/06A23V2002/00A61K38/012A23V2200/326A23V2250/55
Inventor 李敏晶谭成玉孔亮王迪王旭莹
Owner DALIAN OCEAN UNIV
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