Method for separating single fungus spore
A separation method and spore technology, applied in the field of microbial culture, can solve the problems of cumbersome operation, difficulty in making and using glass needles, inability to separate single spores, etc., and achieve simple production and operation, easy reusability, easy disassembly and sterilization Effect
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[0013] The present invention will be described in further detail below in conjunction with accompanying drawing:
[0014] a. According to the size of the spores to be separated, several hard neutral glass capillaries with an inner diameter of 0.1 mm and a length of 100 mm are spared, a glass Pasteur pipette with a slender tip and a rubber head, and an ordinary glass carrier of 25.4×76.2 mm Tablets and 20ml of distilled water, and sterilize the above equipment and distilled water at 121°C for 20 minutes;
[0015] b. Insert the glass capillary from the tip into the Pasteur pipette, leaving a length of 20 mm outside the pipette, and seal the tip of the pipette with Parafilm;
[0016] c. Take a dissecting needle and sterilize it by burning red on the flame of an alcohol lamp. After the dissecting needle cools down, pick some fungal materials to be separated, select Alternaria colonies and Penicillium colonies, and place them in a sterilized On the glass slide, add a drop of steri...
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