Artificial propagation method of diversinervus elegans silvestri

Abstract

The invention discloses an artificial propagation method of diversinervus elegans silvestri. In the method, a pumpkin is used as propagation material, parasaissetia nigra nietner is propagated to be used as an intermediate host, the parasaissetia nigra nietner is used for producing the diversinervus elegans silvestri, the parasaissetia nigra nietner used as the intermediate host is inoculated on the pumpkin and cultured for 35-50 days under indoor natural conditions to inoculate a parasitic wasp; the proportion of the male diversinervus to the female diversinervus is controlled to be 2-4 : 1,the diversinervus are fed with 10% honey water, the proportion of the male encyrtid to the parasaissetia nigra nietner is controlled to be 1 : 5, and after 1-3 days, the pumpkin used as the propagation material is taken out; another pumpkin with the intermediate host is used as the propagation material for propagation of the diversinervus elegans silvestri, the parasaissetia nigra nietner is inoculated on the pumpkin for 1-3 days, and then, the provenances of the diversinervus are discarded after inoculating 1-2 pumpkins; the pumpkin being taken out is placed in the room for cultivation undernatural conditions, the parasitized parasaissetia nigra nietner used as the intermediate host is cut off by a blade so as to collect honeybee pupae, and the grown diversinervus are collected after the eclosion of the pupae. The method can implement volume production of the diversinervus elegans silvestri, and can effectively solve the problems of low progenitive rate and delayed supply and demandof biological control.

Description

technical field [0001] The invention belongs to the field of biological control of agricultural and forestry pests, and in particular relates to an artificial propagation method of the natural enemy insect of the wax scale. Background technique [0002] Coccidae belongs to the class Insecta, Homoptera, Coccoidea. Among the 20 families of Coccidae, Coccidae is the third largest family, second only to Diaspididae and Pseudococcidae. The amount of wax secreted by scale insects is relatively the largest. According to Ben-Dov (1993 ), about 1088 species have been recorded in the undergraduate course, divided into 144 genera. There are 83 species of 39 genera and 83 species known in my country (Tang Fangde, 1991), among which nearly 30 species are common pests of agriculture, forestry, fruit trees and ornamental plants. The important pests of the wax scale family are: red wax scale, orange green wax scale, Japanese wax scale, brown wax scale, jujube ball wax scale, rubber helme...

AI Technical Summary

Problems solved by technology

The above breeding methods are limited to laboratory research, and the production efficiency is low, so it is difficult to mass-produce on a large scale. The material is bulky and requires a relatively closed environment to prevent bees from escaping, making it difficult to inoculate and collect parasitic bees, making it impossible to realize large-scale production; finally, the management of seedlings in the breeding program also increases the difficulty and cost

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