Unlock instant, AI-driven research and patent intelligence for your innovation.

Process for extracting bamboo leaf coarse polysaccharide by supercritical CO2 fluid extraction technology

A supercritical, crude polysaccharide technology, which is applied in medical preparations containing active ingredients, plant raw materials, antitumor drugs, etc., can solve the problems of low extraction rate and no reports.

Inactive Publication Date: 2012-10-31
BEIJING FORESTRY UNIVERSITY
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to supercritical CO 2 Fluid has higher extraction efficiency for non-polar and medium-polar compounds, but the extraction rate for substances with strong polarity and large molecular weight (such as polysaccharides, etc.) is low. In order to improve the selectivity of some polar substances, it is often used in supercritical CO 2 The method of adding a suitable entrainer (or modifier) ​​to the extraction system. Commonly used entrainers include methanol, ethanol, acetone, ethyl acetate, water, etc., and the entrainer is used to improve CO 2 Fluid solubility properties such that supercritical CO 2 It is possible to extract highly polar substances such as polysaccharides
[0005] Although using supercritical CO 2 Fluid extraction technology has been reported to extract some plant polysaccharides (such as shiitake mushrooms, Poria cocos, etc.), but supercritical CO 2 The study of extracting crude polysaccharides from bamboo leaves by fluid extraction technology has not been reported yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process for extracting bamboo leaf coarse polysaccharide by supercritical CO2 fluid extraction technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 .Preparation of crude polysaccharide extract from Phyllostachys pubescens

[0026] Install the kettle lid at one end of the 1000mL extraction kettle, put the folded 3 layers of degreasing gauze into one end of the extraction kettle and flatten it, put 200g of the moso bamboo leaf powder sample crushed to 20 mesh into the extraction kettle, and mix the 600mL volume concentration (v / v ) is 75% ethanol solution into the extraction kettle, put the stacked 3-layer degreasing gauze into the other end of the extraction kettle, and install the lid on the other end, fix the filled extraction kettle in the heating box, and tighten Connect the joints at both ends of the extraction kettle and close the heating box. At this time, all the gas valves in the system should be closed. Open supercritical CO 2 For the power supply of the fluid extraction equipment, set the temperature of the heating box to 40°C. When the temperature of the extraction kettle reaches the set val...

Embodiment 2

[0027] Example 2. Preparation of crude polysaccharide extract from green bamboo leaves

[0028] Install the kettle lid at one end of the 1000mL extraction kettle, put the folded 3 layers of degreasing gauze into one end of the extraction kettle and flatten it, put 200g of the green bamboo leaf powder sample crushed to 20 mesh into the extraction kettle, and mix the 600mL volume concentration (v / v) Pour 75% ethanol solution into the extraction kettle, put the stacked 3-layer degreasing gauze into the other end of the extraction kettle, install the lid on the other end, and fix the filled extraction kettle in the heating box, Tighten the joints at both ends of the extraction kettle, and close the heating box. At this time, all air valves in the system should be closed. Open supercritical CO 2 For the power supply of the fluid extraction equipment, set the temperature of the heating box to 40°C. When the temperature of the extraction kettle reaches the set value, turn on the ...

Embodiment 3

[0029] Example 3. Preparation of Crude Polysaccharide Extract from Bamboo Bamboo Leaves

[0030] Install the kettle lid at one end of the 1000mL extraction kettle, put the folded 3 layers of degreasing gauze into one end of the extraction kettle and flatten it, put 200g of bitter bamboo leaf powder samples crushed to 20 mesh into the extraction kettle, and mix the 600mL volume concentration (v / v ) is 75% ethanol solution into the extraction kettle, put the stacked 3-layer degreasing gauze into the other end of the extraction kettle, and install the lid on the other end, fix the filled extraction kettle in the heating box, and tighten Connect the joints at both ends of the extraction kettle and close the heating box. At this time, all the gas valves in the system should be closed. Open supercritical CO 2 For the power supply of the fluid extraction equipment, set the temperature of the heating box to 50°C. When the temperature of the extraction kettle reaches the set value, ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a process for extracting bamboo leaf coarse polysaccharide by a supercritical CO2 fluid extraction technology, and belongs to the technology of the extraction of bamboo leaf active ingredients. The process comprises the following steps of: putting a crushed bamboo leaf sample into an extraction kettle, and adding ethanol solution as an entrainer; performing static extraction and dynamic extraction at the temperature of between 45 and 50 DEG C under the pressure of between 30 and 50 MPa for 1 to 1.5 hours respectively; and after finishing extracting, concentrating and freeze-drying the obtained extracting solution to obtain the bamboo leaf coarse polysaccharide finally. In the process, an ethanol-water system is used as the entrainer, so that non-pollution operation is realized; and due to the adoption of the supercritical CO2 fluid extraction method in which both the static extraction and dynamic extraction are considered, the extraction rate of the bamboo leaf coarse polysaccharide is high, and CO2 air sources and energy are saved.

Description

technical field [0001] The present invention relates to a kind of preparation technology of bamboo leaf crude polysaccharide, especially relate to a kind of supercritical CO 2 Fluid extraction of crude polysaccharides from bamboo leaves. Background technique [0002] Bamboo is a perennial evergreen plant of the Poaceae (Poaceae) Bambooideae (Bambusoideae). Bamboo leaf polysaccharide is a heteropolysaccharide with a medium relative molecular weight. Its medicinal use began in 1943. Studies have confirmed that bamboo leaf polysaccharides have obvious medicinal effects, and bamboo leaf active polysaccharides have anti-cancer activity, anti-oxidation and immune regulation. [0003] In the past, researchers used water extraction, microwave method, ultrasonic method, etc. to extract bamboo leaf polysaccharides. These traditional extraction methods often have low extraction efficiency, time-consuming, and may lead to unsatisfactory extraction results such as decomposition of bambo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/899C08B37/00A61P35/00A61P37/02A61P39/06A61K127/00
CPCY02P20/54
Inventor 吕兆林任美玲欧阳呓林林西张柏林
Owner BEIJING FORESTRY UNIVERSITY