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Production method for biological source theanine raw material

A production method, the technology of theanine, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problems of inability to obtain tea tree roots, inability to realize large-scale production of biological theanine, etc., to facilitate demonstration and Promote, improve root growth efficiency, and facilitate storage

Inactive Publication Date: 2011-08-17
HUANGSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent with the publication number CN 1308846A discloses a method of aerosol cultivation for tea tree cultivation and can achieve the purpose of producing high-quality and high-yield tea leaves, but it is impossible to obtain tea tree roots that are recycled and contain high theanine content. Therefore, it is also impossible to achieve the purpose of large-scale production of bio-theanine using recycled tea tree roots as raw materials

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  • Production method for biological source theanine raw material
  • Production method for biological source theanine raw material

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The tea tree variety is Shuchazao. The seeds were collected in the tea garden in mid-March, and the experiment began in mid-April. Seed soaking: wash the seeds with tap water for 3 times and then soak them in slow running water for 7 days; seed selection and disinfection: select plump and bottom-sinking seeds and remove them with 0.1% HgCl 2 Soak for 20 minutes to disinfect, then rinse with distilled water for 3 times; Germination: Sow the seeds into a germination pot with 10cm thick perlite, cover with moist perlite, cover with a perforated germination pot lid, and place in a variable temperature seed germination box Medium germination, the daytime temperature is 20°C~30°C, and the night temperature is 15°C~22°C. Spray water appropriately in the future to make the perlite humidity 40%~60%.

[0026]When the tea seed radicle length is 1-2cm, the seedlings are bound with a sponge strip with a width of about 1cm, and the buds and leaves are fixed on the surface of the ae...

Embodiment 2

[0029] The tea tree variety is Keemun Muye species. The seeds were collected in the tea garden in mid-March, and the experiment began in mid-April. Seed soaking: wash the seeds with tap water for 3 times and then soak them in slow running water for 7 days; seed selection and disinfection: select plump and bottom-sinking seeds and remove them with 0.1% HgCl 2 Soak for 20 minutes to disinfect, then rinse with distilled water for 3 times; germination: seeds are sown in a germination pot with 10cm thick perlite, covered with moist perlite, covered with a perforated germination pot lid, and placed in a variable temperature seed germination box For germination, the daytime temperature is 20°C~30°C, and the night temperature is 15°C~22°C. Spray water appropriately in the future to make the perlite humidity 40%~60%.

[0030] When the tea seed radicle length is 1-2cm, the seedlings are bound with a sponge strip with a width of about 1cm, and the buds and leaves are fixed on the aerop...

Embodiment 3

[0033] The tea tree variety is hillside green. The seeds were collected in the tea garden in mid-March, and the experiment began in mid-April. Seed soaking: wash the seeds with tap water for 3 times and then soak them in slow running water for 7 days; seed selection and disinfection: select plump and bottom-sinking seeds and remove them with 0.1% HgCl 2 Soak for 20 minutes to disinfect, then rinse with distilled water for 3 times; Germination: Sow the seeds into a germination pot with 10cm thick perlite, cover with moist perlite, cover with a perforated germination pot lid, and place in a variable temperature seed germination box Medium germination, the daytime temperature is 20°C~30°C, and the night temperature is 15°C~22°C. Spray water appropriately in the future to make the perlite humidity 40%~60%.

[0034] When the tea seed radicle length is 1-2cm, the seedlings are bound with a sponge strip with a width of about 1cm, and the buds and leaves are fixed on the surface of ...

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Abstract

The invention discloses a production method for a biological source theanine raw material. The production method comprises the following steps of: (1) screening and sprouting tea tree seeds; (2) performing aerial fog cultivation on tea seedlings; (3) cutting roots and regenerating root systems; and (4) repeating the step (3) to circularly obtain tea tree roots which are used as the raw material for producing theanine, wherein aerial fog nutrient solution in the step (2) comprises C2H7N or C2H8ClN or C3H7NO2 and C5H9NO4 or C5H10N2O3 besides nutrients necessary to the growth of the tea seedlings, and the pH of the aerial fog nutrient solution is between 5 and 5.5. By the production method, the raw material tea tree roots for producing the theanine can be obtained throughout the year, and a special formula of the tea tree growth nutrient solution is used to effectively improve the content of the theanine in the tea tree root systems; and the tea seedlings obtained by the aerial fog cultivation can be repeatedly harvested and repeatedly grow so as to realize industrial operation for the production of the tea tree roots throughout the year.

Description

[0001] technical field [0002] The invention relates to a production method of biological source theanine raw material. Background technique [0003] Theanine is a characteristic non-protein amino acid of tea tree. It is a white needle-like body, easily soluble in water, and has a sweet and fresh taste. It is a component of the taste of tea and is also widely used to improve the flavor of food. Modern medicine has confirmed that theanine has many health care effects on the human body, especially that theanine can calm the nerves without toxic side effects, and is an ideal drug for treating depression. [0004] At present, the sources of biological theanine include extraction and separation of theanine from tea leaves or waste liquid for preparing tea polyphenols, cell culture methods, hairy root culture methods, microbial fermentation methods, etc. Extracting theanine from tea leaves is not only costly, but also competes with commercial tea production for raw materials; ...

Claims

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Application Information

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IPC IPC(8): A01G7/06C07C237/06
Inventor 马雪泷房江育
Owner HUANGSHAN UNIV
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