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Method for extracting Dicranopteris dichotoma total flavone under assistance of microbial-fermentation self-made mixed enzymes

A technology of microbial fermentation and assisted extraction, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems such as flavonoids of strychnids that have not yet been seen, and achieve short extraction time, low cost and stable enzyme source. Effect

Inactive Publication Date: 2011-08-31
GUANGDONG UNIV OF TECH
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  • Application Information

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Problems solved by technology

[0005] So far, there has been no report on the direct application of the compound enzyme liquid prepared by microbial fermentation to the enzymatic hydrolysis method to assist the extraction of Osmanthus flavonoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take an appropriate amount of Aspergillus niger to activate, expand the culture, connect it to the slant medium of Chase, and cultivate it for 3 days; wash the spores on the slant with sterile water, transfer them to sterile water with glass beads, and shake to prepare the spores Suspension, inoculated in sterilized solid fermentation medium, solid fermentation basal medium (bran 10g, water 15ml, pH naturally in a 250ml Erlenmeyer flask), cultured at 36°C for 72 hours, metabolized to produce complex enzymes. Add water to the solid fermented bacteria and culture medium to extract the compound enzyme liquid, and set aside. Add 2.5ml of compound enzyme to the soaking solution containing 5g of Osmanthus punctatus and 100ml of sterile water, the extraction temperature is 80°C, the pH value is 5, the extraction time is 2h, and centrifugal suction filtration is obtained to obtain the total flavonoids extract of Osmanthus lanceolata. Taking rutin as a reference, the extraction ...

Embodiment 2

[0027] Take an appropriate amount of Aspergillus oryzae strains to activate, expand the culture, connect to Cha's slant medium, and cultivate for 3 days; wash the spores on the slant with sterile water, transfer them to sterile water with glass beads, and shake to prepare spores Suspension, inoculated in sterilized solid fermentation medium, solid fermentation basal medium (bran 10g, water 15ml, pH naturally in a 250ml Erlenmeyer flask), cultured at 36°C for 72 hours, metabolized to produce complex enzymes. Dry the solid fermented bacteria and their culture base at 60°C, crush them, and store them as powdered dry bacteria. Temporary preparation of enzyme solution when needed: Weigh powdered dry bacteria, add water to extract the compound enzyme solution, and store it at 4°C for later use. Add 3.5ml of compound enzyme to the soaking solution containing 5g of Osmanthus punctatus and 100ml of sterile water, the extraction temperature is 80°C, the pH value is 5, the extraction tim...

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Abstract

The invention provides a method for extracting Dicranopteris dichotoma total flavone under assistance of microbial-fermentation self-made mixed enzymes, which belongs to the field of total flavone extracting methods and mainly solves a problem of low total flavone extracting rate. The method comprises the processes of: activating microbial strains; carrying out expanded culture; preparing a complex enzyme for the future use by using a solid fermentation method; adding 1-5 ml of complex enzyme to a Dicranopteris dichotoma soak solution in a specific solid-liquid ratio; extracting for 0.5-5 hours at 40-70 DEG C and with the pH value of 4-8; and centrifuging and leaching to obtain a Dicranopteris dichotoma total flavone extracting solution. The total flavone extracting method provided by the invention has the advantages of low cost, short extraction time, low extraction temperature, no use of organic solvent, friendly operation environment and high one-time extraction rate. The method is suitable for scientific researches and experiments and industrial production and is worthy of popularization.

Description

technical field [0001] The invention relates to the field of a method for extracting total flavonoids, in particular to a method for assisting the extraction of total flavonoids of Osmanthus flavonoids by using self-made mixed enzymes by microbial fermentation. technical background [0002] At present, there are direct extraction methods for extracting total plant flavonoids: hot water extraction, lye extraction, organic solvent extraction, supercritical fluid extraction; auxiliary extraction methods: microwave-assisted extraction, ultrasonic-assisted extraction, enzymatic hydrolysis assisted extraction, etc. The active ingredients of plants, flavonoids, are often wrapped in the cell wall. Most flavonoids are slightly soluble in water, and flavonoid glycosides are soluble in water. The plant cell wall is composed of cellulose, etc., so that the flavonoids in the cell wall To be captured by the extractant to the maximum extent, the cell wall must be broken. [0003] The ass...

Claims

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Application Information

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IPC IPC(8): A61K36/11C12N9/00C12R1/685C12R1/69
Inventor 丁利君孙俊熊俊娟
Owner GUANGDONG UNIV OF TECH
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