A kind of method that immobilized enzyme catalyzes to produce citicoline sodium

A technology of citicoline sodium and immobilized enzyme, which is applied in the field of immobilized enzyme catalyzed production of citicoline sodium, which can solve the problems of enzymatic synthesis, low reaction conversion rate, and low product concentration, etc., and achieve reduction Effects of synthesis process time, environmental friendliness, and mild reaction conditions
CN103849666BActive Publication Date: 2015-09-02KAIPING GENUINE BIOCHEM PHARMA
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Patents(China)
Current Assignee / Owner
KAIPING GENUINE BIOCHEM PHARMA
Publication Date
2015-09-02

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Abstract

The invention provides a method for catalytically producing citicoline sodium with an immobilized enzyme. The method comprises the following steps: by utilizing engineered escherichia coli of a molecularly cloned cytidine phosphotransferase gene for fermentation, preparing cytidine phosphotransferase, and preparing cytidine phosphotransferase liquid through purification; immobilizing cytidine phosphotransferase; by using phosphorylcholine and cytidine disodium triphosphate as raw materials, catalytically generating citicoline with immobilized cytidine phosphotransferase. The method is simple in production process, short in production cycle and low in production cost and can be widely applied to industrial production of citicoline.
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Description

Technical field:

[0001] The invention relates to the field of pharmacy, in particular to a method for catalyzing the production of citicoline sodium by immobilized enzymes. Background technique:

[0002] Citicoline sodium, also known as citicoline sodium, the chemical name is the monosodium salt of choline cytosine nucleoside diphosphate, a brain metabolism activator, which is a nucleoside derivative and is a derivative of phosphatidylcholine. The precursor substance is a coenzyme necessary for the synthesis of lecithin. It was discovered in 1954, synthesized in 1956, and successfully produced in China in 1973.

[0003] The pharmacological effects of citicoline sodium mainly include: it can restore the structure of the cell membrane after brain cell injury, enhance the function of the cell membrane, improve the function of neurons; reduce cerebrovascular resistance, increase cerebral blood flow, promote brain metabolism, improve cerebral circulation, Improve brain function;...

Claims

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