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A method employing Enterococcus faecalis fermentation for preparing chitin from exoskeletons of crustaceans

A technology of crustaceans and Enterococcus faecalis, applied in the biological field, to achieve the effect of reducing the use of strong acid and alkali and reducing pollution

Inactive Publication Date: 2013-05-01
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The report of producing chitin by fermentation of Enterococcus faecalis has not yet been seen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for preparing chitin from crustacean exoskeleton by fermentation of Enterococcus faecalis, comprising the following steps:

[0019] The first step is to crush 100g of shrimp shells, add 100g of water and 10g of glucose, and mix and stir;

[0020] In the second step, Enterococcus faecalis is inserted into the activation medium for activation, and then cultured at a constant temperature at 37°C for 24 hours, and then inserted into the mixed solution of the first step according to the inoculum amount of 10%;

[0021] The third step is to ferment the mixed solution of Enterococcus faecalis at 27°C for 48 hours at a constant temperature;

[0022] In the fourth step, after the fermentation broth and the fermentation residue are separated by filtration, the fermentation residue is soaked in dilute hydrochloric acid with a concentration of 0.5 mol / L for 6 hours, and then decolorized with hydrogen peroxide to obtain chitin.

Embodiment 2

[0024] A method for preparing chitin from crustacean exoskeleton by fermentation of Enterococcus faecalis, comprising the following steps:

[0025] In the first step, crush 100g of crab shells, add 300g of water and 20g of sucrose, and mix and stir;

[0026] In the second step, Enterococcus faecalis is inserted into the activation medium for activation, and then cultured at a constant temperature at 37°C for 36 hours, and then inserted into the mixed solution of the first step according to the inoculum amount of 20%;

[0027] The third step is to ferment the mixed solution of Enterococcus faecalis at 45°C for more than 48 hours at a constant temperature;

[0028] In the fourth step, after the fermentation broth and the fermentation residue are separated by filtration, the fermentation residue is soaked in dilute hydrochloric acid with a concentration of 5 mol / L for 4 hours, and then decolorized with hydrogen peroxide to obtain chitin.

Embodiment 3

[0030] A method for preparing chitin from crustacean exoskeleton by fermentation of Enterococcus faecalis, comprising the following steps:

[0031] The first step is to pulverize the mixture of shrimp shells and crab shells with a total weight of 100g, add 200g of water and 15g of glucose, and mix and stir;

[0032] In the second step, enter the Enterococcus faecalis into the activation medium for activation, and then culture it at a constant temperature at 37°C for 30 hours, and then insert it into the mixed solution of the first step according to the inoculation amount of 15%;

[0033] The third step is to ferment the mixed solution of Enterococcus faecalis at 35°C for more than 48 hours at a constant temperature;

[0034] The fourth step is to filter and separate the fermentation broth from the fermentation residue, soak the fermentation residue in dilute hydrochloric acid with a concentration above 2 mol / L for 2 hours, and decolorize with hydrogen peroxide to obtain chitin...

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PUM

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Abstract

The invention provides a method employing Enterococcus faecalis fermentation for preparing chitin from exoskeletons of crustaceans. The method comprises the steps of firstly crushing the exoskeletons of the crustaceans, adding water and glucose or cane sugar, mixing and stirring; secondly, inoculating the Enterococcus faecalis to an activation medium for activation, culturing at a constant temperature and inoculating to the mixed liquid in the first step; thirdly, allowing the mixed liquid inoculated with the Enterococcus faecalis to ferment at a constant temperature; and finally, separating fermentation liquor and fermentation residue through filtering, soaking the fermentation residue with concentrated or diluted hydrochloric acid, and decolorizing for obtaining the chitin. The method for preparing the chitin enables effective utilization of all final products and reduces pollution.

Description

technical field [0001] The invention belongs to the field of biological technology, relates to a preparation method of chitin, in particular designs a method for preparing chitin from exoskeleton of crustaceans by fermenting Enterococcus faecalis. Background technique [0002] Chitin, also known as chitin and chitin, is a linear polymer with β-1,4-N-acetyl-D-glucosamine as the basic unit. Chitin is widely found in the crustaceans of shrimps and crabs, the carapaces of insects, and the cell walls of fungi (yeast, mold), algae, and plants. It is one of the most abundant organic substances on the earth. %-30%. [0003] Chitin and its degradation products chitooligosaccharide, chitosan and N-acetylglucosamine have anticancer, antibacterial, immune regulation and plant growth effects, and have broad application prospects and Economic Value. At present, chitin is mainly extracted from shrimp and crab shells. Because the chitin in shrimp and crab shells is covalently bonded to ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/46
Inventor 缑敬轩董文宾曾桥
Owner SHAANXI UNIV OF SCI & TECH
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