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Biological assay method and device for detecting toxicity potency of aconite traditional Chinese medicines

A bioassay, aconitum technology, applied in pharmaceutical formulations, preparations for in vivo tests, etc., can solve problems that are not conducive to the comparison of the toxicity of medicinal materials and safety control, difficult to characterize the overall efficacy or toxicity of drugs, and unsatisfactory Production and clinical needs, etc., to achieve the effect of intuitive and clear drug toxicity, convenient and fast injection process, and improved efficiency and accuracy of results

Inactive Publication Date: 2012-07-18
THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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AI Technical Summary

Problems solved by technology

However, the current safety control methods and standards for aconitum medicinal materials have many limitations and cannot meet the needs of production and clinical practice.
Traditional Chinese medicine itself is a complex system with multi-component and multi-target characteristics. Generally, there are many interactions between different components in terms of activity, such as synergy and antagonism. It is often difficult to characterize the overall efficacy of a drug by simply measuring a few specific components. or toxicity
For example, there are many types of toxic substances in aconitum medicinal materials. In addition to diester alkaloids, other alkaloids are also toxic. The content of the three diester alkaloids cannot accurately represent the overall toxicity of medicinal materials
The pharmacological experiments that have been carried out also show that the toxicity of the total extract of the medicinal material is far greater than the sum of the toxicity of the three diester alkaloids, and there are even three types of diester alkaloids with low content but high total toxicity among different batches of medicinal materials. Phenomena, which will bring great risks to clinical drug use
Secondly, the determination of the contents of the three diester-type alkaloids cannot intuitively explain the toxicity of medicinal materials, which is not conducive to the comparison of the toxicity of different medicinal materials and safety control

Method used

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  • Biological assay method and device for detecting toxicity potency of aconite traditional Chinese medicines
  • Biological assay method and device for detecting toxicity potency of aconite traditional Chinese medicines
  • Biological assay method and device for detecting toxicity potency of aconite traditional Chinese medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] Test product: two batches of Baifupian 1 and 2 processed from raw aconite

[0039] Standard product: aconitine, assigned value 1mg=1000u, u is unit number

[0040] Animal: SD rat, male, 0.2±0.02kg

[0041] Proceed as follows:

[0042] a. Accurately weigh 10 mg of aconitine and place it in a 10 ml volumetric flask, add absolute ethanol to the mark, seal tightly, shake to dissolve completely, and store at 4°C. During the determination, accurately measure 0.5 ml of aconitine solution and place it in a 100 ml volumetric flask, and dilute to the mark with 0.9% sodium chloride solution to obtain aconitine dilution.

[0043] b. Accurately weigh 10g of the powders of Baifu Tablet 1 and Baifu Tablet 2 (passed through a 100-mesh sieve), place them in two ground-mouth conical flasks, add 100ml of 70% ethanol respectively, soak for 30 minutes, and sonicate 30 minutes. The extract was filtered with a 0.2 μm microporous membrane, and the filtrate was concentrated to 10 ml to obta...

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Abstract

The invention relates to a biological assay method for detecting the toxicity potency of aconite traditional Chinese medicines, which can accurately and rapidly detect the whole toxicity of the aconite traditional Chinese medicines, and can mark the toxicity potency of each medicinal material in a unified manner. The invention also relates to a device applicable to the biological assay method fordetecting the toxicity potency of aconite traditional Chinese medicines.

Description

technical field [0001] The invention relates to a bioassay method and device for detecting the toxicity potency of aconitum traditional Chinese medicine. Background technique [0002] Aconitum traditional Chinese medicines such as Fuzi, Chuanwu and Caowu are widely used clinically, especially Fuzi has played a prominent role in the rescue of critical illnesses and the treatment of difficult cases that cannot be cured for a long time. One product. Aconitum traditional Chinese medicines have a common feature, that is, they are highly toxic, the therapeutic dose is close to the lethal dose, and the safety window is narrow, which brings great challenges to clinical safe drug use. For example, aconite is called "the most useful and the most difficult to use". "Chinese medicine. Diester-type alkaloids are the main toxic components of aconitine traditional Chinese medicine, mainly including aconitine, neoaconitine and hypoaconitine, which are extremely toxic, mainly causing serio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 肖小河王伽伯秦谊山丽梅金城
Owner THE FIFTH MEDICAL CENT OF CHINESE PLA GENERAL HOSPITAL
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