Nucleic acid hybridization membrane strip, polymerase chain reaction (PCR) primer and kit used for diagnosing G6PD (Glucose-6-phosphate Dehydrogenase) deficiency diseases

A nucleic acid hybridization and deficiency technology, which is applied in DNA/RNA fragmentation, recombinant DNA technology, and the determination/examination of microorganisms. strong effect

Active Publication Date: 2011-09-07
SHENZHEN YILIFANG BIOTECH CO LTD
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  • Claims
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Problems solved by technology

[0003] At present, commonly used clinical G6PD deficiency detection methods include methemoglobin reduction test, G6PD activity test, G6PD quantitative ratio method, etc. These chemical test methods are simple in process and low in price, and are suitable

Method used

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  • Nucleic acid hybridization membrane strip, polymerase chain reaction (PCR) primer and kit used for diagnosing G6PD (Glucose-6-phosphate Dehydrogenase) deficiency diseases
  • Nucleic acid hybridization membrane strip, polymerase chain reaction (PCR) primer and kit used for diagnosing G6PD (Glucose-6-phosphate Dehydrogenase) deficiency diseases
  • Nucleic acid hybridization membrane strip, polymerase chain reaction (PCR) primer and kit used for diagnosing G6PD (Glucose-6-phosphate Dehydrogenase) deficiency diseases

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Embodiment Construction

[0023] The present invention will be described in detail below with reference to the accompanying drawings and in combination with preferred specific embodiments.

[0024] 1. Preparation of 12 mutation detection probes and 9 normal control probes

[0025] 21 probes were synthesized according to the sequences in Table 1 and Table 2. The 3' or 5' ends of the mutation detection probes and / or normal control probes were labeled with amino groups. The synthetic method was the existing conventional DNA synthesis method.

[0026] Table 1:

[0027]

[0028] Table 2:

[0029]

[0030] Note: "M" stands for mutation detection probe, "N" stands for normal control probe.

[0031] Each of the mutation detection probes corresponds to a G6PD gene mutation site, and the G6PD gene mutation sites are cDNA95, cDNA392, cDNA487, cDNA493, cDNA592, cDNA871, cDNA1004, cDNA1024, cDNA1376, cDNA1381, cDNA1387, cDNA1388; One of the mutation detection probes comprises a sequence of 15-20 bases, an...

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Abstract

The invention discloses a nucleic acid hybridization membrane strip used for diagnosing G6PD (Glucose-6-phosphate Dehydrogenase) deficiency diseases, comprising a substrate on which specific mutation detection probes are fixed; each mutation detection probe corresponds to a G6PD gene mutational site; the G6PD gene mutational site is at least one of cDNA95, cDNA392, cDNA487, cDNA493, cDNA592, cDNA871, cDNA1004, cDNA1024, cDNA1376, cDNA1381, cDNA1387 and cDNA1388; each mutation detection probe comprises 15-20 base sequences; and the middle position in the sequence comprises mutation base of theG6PD gene corresponding to the middle position of the sequence. The nucleic acid hybridization membrane strip has the advantages of high accuracy and strong specificity, and capacity of detecting a gene recessive carrier and the like.

Description

technical field [0001] The present invention relates to a nucleic acid hybridization membrane strip for medically diagnosing human glucose-6-phosphate dehydrogenase (glucose-6-phosphate dehydrogenase, abbreviated as G6PD) deficiency. Polymerase Chain Reaction (PCR) primers for amplifying gene fragments of samples to be tested; the invention also relates to a kit for diagnosing glucose-6-phosphate dehydrogenase deficiency. Background technique [0002] G6PD deficiency is the most common X-linked incomplete dominant genetic disease in the world, and it is estimated that 400 million people are affected worldwide. The high-incidence areas of the disease include the Mediterranean coast, Africa, Southeast Asia and southern provinces of my country. Clinically, G6PD deficiency manifests as diseases such as neonatal jaundice, favism, drug-induced hemolysis, infectious hemolysis, and non-spherical cell hemolytic anemia. Serious, if not treated in time, it will endanger the life of t...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 危梅娟
Owner SHENZHEN YILIFANG BIOTECH CO LTD
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