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Heat-resistance microencapsulated probiotics and preparation method and use thereof

A technology of probiotics and microcapsules is applied in the field of heat-resistant microcapsule probiotics and their preparation, which can solve the problems such as insufficient environmental resistance of probiotics and restrict development, and achieve the effects of saving money, avoiding wastage and prolonging shelf life.

Active Publication Date: 2011-09-14
BIOGROWING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the insufficient environmental resistance of probiotics limits their development in the field of additives

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation method of heat-resistant microcapsule probiotics comprises the following steps:

[0033] a. Probiotics are inoculated, fermented and cultivated so that the concentration of live bacteria in the fermented liquid reaches 10 10 cfu / g;

[0034] b. centrifuging the fermented liquid in step a at a temperature of 4°C and a rotational speed of 2500-4000 rpm to obtain wet probiotic bacteria;

[0035] c. Mix 9% β-cyclodextrin and 8% hydroxypropyl methylcellulose in terms of the weight percentage of probiotic wet cells and dissolve them in hot water at 60°C, stir to fully dissolve to obtain a solution and cool down to 40°C;

[0036] d. Add the probiotic wet cells to the solution for the first embedding, and gently stir for 10 minutes at the speed of the disperser at 6000-8000rpm to fully mix to obtain the first embedding;

[0037] e. For the first emulsification and homogenization of the embedded material twice, the emulsification and homogenization pressure for...

Embodiment 2

[0043] The preparation method of heat-resistant microcapsule probiotics comprises the following steps:

[0044] a. Probiotics are inoculated, fermented and cultivated so that the concentration of live bacteria in the fermented liquid reaches 10 10 cfu / g;

[0045] b. centrifuging the fermented liquid in step a at a temperature of 4°C and a rotational speed of 2500-4000 rpm to obtain wet probiotic bacteria;

[0046] c. Mix 6% β-cyclodextrin and 7% hydroxypropyl methylcellulose in terms of the weight percentage of the probiotic wet cells and dissolve them in hot water at 70°C, stir to fully dissolve to obtain a solution and cool down to 45°C;

[0047] d. Add the probiotic wet cells to the solution, carry out the first embedding, stir lightly for 10 minutes at the speed of the disperser at 6000-8000rpm, and fully mix to obtain the first embedding;

[0048] e. Mechanically emulsify and homogenize the embedding material twice for the first time, the first emulsification and homog...

Embodiment 3

[0054] The preparation method of heat-resistant microcapsule probiotics comprises the following steps:

[0055] a. Probiotics are inoculated, fermented and cultivated so that the concentration of live bacteria in the fermented liquid reaches 10 10 cfu / g;

[0056] b. centrifuging the fermented liquid in step a at a temperature of 4°C and a rotational speed of 2500-4000 rpm to obtain wet probiotic bacteria;

[0057]c. Mix 5% β-cyclodextrin and 3% hydroxypropyl methylcellulose in terms of the weight percentage of the probiotic wet cells and dissolve them in hot water at 65°C, stir to fully dissolve to obtain a solution and cool down to 35°C;

[0058] d. Add the probiotic wet cells to the solution for the first embedding, and gently stir for 10 minutes at the speed of the disperser at 6000-8000rpm to fully mix to obtain the first embedding;

[0059] e. Mechanically emulsify and homogenize the embedding for the first time twice, the first emulsification and homogenization pressu...

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Abstract

The invention discloses heat-resistance microencapsulated probiotics. In the microencapsulated probiotics, the grain size is between 20 and 400 mu m, the embedding rate is more than or equal to 90 percent, the number of active bacteria is 10<9> to 10<10>cfu / g, the dissolution in cold water is instant, and the number of active bacteria after the heat-resistance microencapsulated probiotics are placed at 80 to 90 DEG C for 3 minutes is reduced by 1 to 1.5 orders of magnitude. The invention also discloses a preparation method for the heat-resistance microencapsulated probiotics. The heat-resistance microencapsulated probiotic product provided by the invention has high stress resistance and stability; the production process provided by the invention is simple, low in product cost, is universally applicable to probiotics; and the embedding rate of the prepared microcapsules is high, and the microcapsules are suitable to be used as additives for foods, health-care products, medicines or feed.

Description

technical field [0001] The invention relates to the field of microbial additives, and relates to a heat-resistant microcapsule probiotic, a preparation method thereof and an application as an additive. Background technique [0002] Since the early 1990s, all kinds of "probiotics" health care products have swept the whole world. At the same time, the research on "probiotics" has become a hot research topic in the world. The definition generally accepted by the international nutrition community is: probiotics are a kind of bacteria that are beneficial to animals, and they can be directly taken as food additives to maintain the balance of intestinal flora. So far, the probiotics that scientists have discovered can be roughly divided into three categories, including: ① Lactobacillus (such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus jannasii, Lactobacillus Lamansii, etc.); Bacilli (such as Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium ovale...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23K1/16A23P1/04A61K35/74A61K9/50A23K10/18A23K40/30A23L33/135A61K35/744A61K35/745A61K35/747
Inventor 王翀刘帅宋锦安闫屹泓
Owner BIOGROWING CO LTD
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