Heat-resistance microencapsulated probiotics and preparation method and use thereof

A technology of probiotics and microcapsules is applied in the field of heat-resistant microcapsule probiotics and their preparation, which can solve the problems such as insufficient environmental resistance of probiotics and restrict development, and achieve the effects of saving money, avoiding wastage and prolonging shelf life.

Active Publication Date: 2011-09-14
BIOGROWING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the insufficient environmental resistance of pro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1

[0032] The preparation method of heat-resistant microcapsule probiotics includes the following steps:

[0033] a. Inoculate and ferment the probiotics to make the concentration of viable bacteria in the fermentation broth reach 10 10 cfu / g;

[0034] b. Centrifuge the fermentation broth of step a at a temperature of 4°C and a rotation speed of 2500-4000 rpm to obtain wet probiotic bacteria;

[0035] c. Mix 9% of β-cyclodextrin and 8% of hydroxypropyl methylcellulose based on the weight percentage of the probiotic wet cells and dissolve them in hot water at 60°C, stir to fully dissolve the solution and cool down To 40°C;

[0036] d. Add the wet probiotic bacteria to the dissolving solution, carry out the first embedding, and lightly stir for 10 minutes under the condition of the speed of the disperser at 6000-8000 rpm to fully dissolve the first embedding;

[0037] e. The first time the embedded material is mechanically emulsified and homogenized twice, the first emulsi...

Example Embodiment

[0042] Example 2

[0043] The preparation method of heat-resistant microcapsule probiotics includes the following steps:

[0044] a. Inoculate and ferment the probiotics to make the concentration of viable bacteria in the fermentation broth reach 10 10 cfu / g;

[0045] b. Centrifuge the fermentation broth of step a at a temperature of 4°C and a rotation speed of 2500-4000 rpm to obtain wet probiotic bacteria;

[0046] c. Mix 6% β-cyclodextrin and 7% hydroxypropyl methylcellulose based on the weight percentage of the probiotic wet cells and dissolve them in 70°C hot water, stir to fully dissolve the solution and cool down To 45°C;

[0047] d. Add the wet probiotic bacteria to the dissolving solution, carry out the first embedding, and lightly stir for 10 minutes under the condition of the speed of the disperser at 6000-8000 rpm to fully dissolve the first embedding;

[0048] e. The first time the embedded material is mechanically emulsified and homogenized twice, the first emulsification ...

Example Embodiment

[0053] Example 3

[0054] The preparation method of heat-resistant microcapsule probiotics includes the following steps:

[0055] a. Inoculate and ferment the probiotics to make the concentration of viable bacteria in the fermentation broth reach 10 10 cfu / g;

[0056] b. Centrifuge the fermentation broth of step a at a temperature of 4°C and a rotation speed of 2500-4000 rpm to obtain wet probiotic bacteria;

[0057] c. Mix 5% of β-cyclodextrin and 3% of hydroxypropyl methylcellulose based on the weight percentage of probiotic wet cells and dissolve them in 65°C hot water, stir to fully dissolve the solution and cool down To 35°C;

[0058] d. Add the wet probiotic bacteria to the dissolving solution, carry out the first embedding, and lightly stir for 10 minutes under the condition of the speed of the disperser at 6000-8000 rpm to fully dissolve the first embedding;

[0059] e. The first time the embedded material is mechanically emulsified and homogenized twice, the first emulsificatio...

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Abstract

The invention discloses heat-resistance microencapsulated probiotics. In the microencapsulated probiotics, the grain size is between 20 and 400 mu m, the embedding rate is more than or equal to 90 percent, the number of active bacteria is 10<9> to 10<10>cfu/g, the dissolution in cold water is instant, and the number of active bacteria after the heat-resistance microencapsulated probiotics are placed at 80 to 90 DEG C for 3 minutes is reduced by 1 to 1.5 orders of magnitude. The invention also discloses a preparation method for the heat-resistance microencapsulated probiotics. The heat-resistance microencapsulated probiotic product provided by the invention has high stress resistance and stability; the production process provided by the invention is simple, low in product cost, is universally applicable to probiotics; and the embedding rate of the prepared microcapsules is high, and the microcapsules are suitable to be used as additives for foods, health-care products, medicines or feed.

Description

technical field [0001] The invention relates to the field of microbial additives, and relates to a heat-resistant microcapsule probiotic, a preparation method thereof and an application as an additive. Background technique [0002] Since the early 1990s, all kinds of "probiotics" health care products have swept the whole world. At the same time, the research on "probiotics" has become a hot research topic in the world. The definition generally accepted by the international nutrition community is: probiotics are a kind of bacteria that are beneficial to animals, and they can be directly taken as food additives to maintain the balance of intestinal flora. So far, the probiotics that scientists have discovered can be roughly divided into three categories, including: ① Lactobacillus (such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus jannasii, Lactobacillus Lamansii, etc.); Bacilli (such as Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium ovale...

Claims

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Application Information

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IPC IPC(8): A23L1/30A23K1/16A23P1/04A61K35/74A61K9/50A23K10/18A23K40/30A23L33/135A61K35/744A61K35/745A61K35/747
Inventor 王翀刘帅宋锦安闫屹泓
Owner BIOGROWING CO LTD
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