High-efficiency non-injection type transmission method for live vaccine

An injection-type vaccine technology, applied in the field of vaccinology, can solve the problem of low immune effect and achieve the effect of simple application, low price and high protection rate

Inactive Publication Date: 2011-09-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immune effect of the reported oral vaccines delivered by microsphere ca

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Routine single injection delivery of vaccines

[0019] Step 1) Immunization injection of vaccine. Cultivate Edwardsiella tarda vaccine strain 15947 (purchased from ATCC, USA) at 28°C in LB medium to OD 600 0.8-1, and then centrifuged (5000g, 4°C) for 10min. Collect the cells and suspend them in PBS to a final concentration of 1x10 8 cfu / ml, that is, 15947 suspension. 50 flounder were randomly divided into 2 groups (25 in each group), named A and B respectively. The fish in groups A and B were injected intraperitoneally with 15947 suspension or PBS (control group), and the injection volume of each fish was 100ul.

[0020] The LB composition is by weight percentage: 1.0% peptone, 0.5% yeast powder, 1.0% sodium chloride, 97.5% distilled water; the PBS composition is by weight percentage: 0.8% NaCl, 0.02% KCl, 0.358% Na 2 HPO 4 .12H 2 O, 0.024% NaH 2 PO 4 , 98.798% distilled water.

[0021] Step 2) Detection of the immune protection effect of the vaccine. Cultiva...

Embodiment 2

[0024] oral delivery of vaccines

[0025] Step 1) Preparation of vaccine sodium alginate microsphere feed and control sodium alginate microsphere feed. Cultivate Edwardsiella tarda vaccine strain 15947 to OD in LB medium at 28 °C 600 0.8-1, and then centrifuged (5000g, 4°C) for 10min. Collect the cells and suspend them in normal seawater to a final concentration of 1x10 9 cfu / ml, that is, 15947 suspension. Mix 50ml of sodium alginate (3%) with 30ml of 15947 suspension or PBS (control), then add 2000ml of paraffin, 10ml of S-80 emulsifier (all purchased from Sigma, USA) and 50ml of calcium chloride (0.15M) ( purchased from Shanghai Sangon Company); the mixed solution containing 15947 and PBS contrast was centrifuged (1000g, 10min) respectively, and the precipitate was collected; ) and mixed with F(II)-26. Twin-screw extruder granulator (South China University of Technology, Guangzhou). Processed into pellet feed (1.5mm×2.0mm), namely vaccine sodium alginate microsphere feed...

Embodiment 3

[0029] Immersion transfer of vaccines

[0030] Step 1) Preparation of vaccine soaking solution. Grow vaccine strain 15947 to OD in LB medium at 28 °C 600 0.8-1, and then centrifuged (5000g, 4°C) for 10min. Collect the cells and suspend them in normal seawater to a final concentration of 1x10 8 cfu / ml is the vaccine soaking solution.

[0031] Step 2) Immunization delivery of the vaccine. Fifty flounder were randomly divided into 2 groups (25 in each group), named E (sample group) and F (control group). Soak the fish of group E in the vaccine soaking solution of the above step 1) for 1 hour, and soak the fish of group F in ordinary sea water for 1 hour.

[0032] Step 3) Detection of the immune protection effect of the vaccine. Cultivation of Edwardsiella tarda TX1 to OD in LB medium 600 0.7, and then centrifuged (5000g, 4°C, 10min). Collect the cells and suspend them in PBS to a final concentration of 5x10 8 cfu / ml is the suspension of Edwardsiella tarda. On the 30th d...

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Abstract

The invention relates to the field of vaccinology, in particular to a high-efficiency non-injection type transmission method for a live vaccine. The transmission method comprises the steps of: making vaccines into an oral vaccine and an immersion vaccine; and transmitting the oral vaccine and the immersion vaccine by a superposition method. The vaccine transmission method avoids the limitation of the conventional injection method, is simple and practicable, has relatively low manufacture cost, has an unlimited application range, and has nearly the same high-efficiency immune effect as the injection method.

Description

technical field [0001] The invention relates to the field of vaccinology, in particular to a high-efficiency non-injection delivery method for live bacterial vaccines. Background technique [0002] Disease is one of the main bottlenecks restricting the development of aquaculture industry in my country. The prevention and control of aquaculture diseases can start from many aspects, among which vaccine immunization is one of the most effective means of fish disease prevention and control generally recognized in the world today. Vaccine delivery (i.e. vaccine introduction into animal body) methods can be simply divided into several categories, namely injection type, soaking type and oral type. The advantage of vaccine delivery by injection is that it has a good immune effect, but its disadvantage is that it is expensive and difficult to apply on a large scale. The advantages of soaking and oral delivery of vaccines are low price, simple application, and wide application, but ...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K9/16A61K47/36A61P31/04
Inventor 孙黎胡永华程爽
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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