Method for rapidly measuring nuclear DNA content

Abstract

The invention relates to a method for rapidly measuring nuclear DNA content. The method comprises the following steps: Feulgen dyeing is performed on the nucleus, then EA50 or eosin is used to dye the cytoplasm, after two-step dyeing, a computer image analysis system is adopted to scan a sample on a glass slide, the grayness is obtained to perform registration with a color image; the cells in the field of vision are classified according to the morphological characteristics of the cells, normal lymphocytes are selected as the standard cells for calculating the DNA content; and the epithelial cells in each field of vision are selected, the DNA content of each epithelial cell on the glass slide is calculated according to the DNA content of the standard cells, the positions of the epithelial cells are recorded, and a cell picture is stored. A grayscale image and a color image in the same area of field of vision are provided for the analysis of the computer image analysis system, and the color image is provided for morphological observation and physician review. By adopting the method, the nuclear DNA content can be measured rapidly and the clinician can observe the cell morphology conveniently.

Description

technical field [0001] The invention belongs to the field of nucleotide detection, in particular to a method for quickly measuring the DNA content in the nucleus. Background technique [0002] When the cells of an organism become cancerous, the DNA content in the nucleus increases. Therefore, it can be judged whether a cell is cancerous according to whether the DNA content in the nucleus increases significantly. The determination of DNA in the nucleus of organisms is mostly determined by optical methods. The general method is to specifically combine the dye or chromogen with the DNA in the nucleus, and measure the optical density in the nucleus under light. When the DNA content in the nucleus increases, the DNA-binding dye or chromogenic agent increases, and its optical density increases. Using this principle, the DNA content in the nucleus can be measured by image analysis to determine whether the cell is cancerous. In addition, when clinicians examine cells under a mic...

AI Technical Summary

Problems solved by technology

Because of this, there are certain differences in the diagnosis of different doctors.

At the same time, there are many cells in each sample, and a large number of fields of view must be observed under the microscope to read a slide, which is prone to visual fatigue when the sample size is large

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