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Preparation method of total glucosides from Paeonia lactiflora Pall., and Paeonia lactiflora Pall. total glucoside preparation and use thereof

A technology of total glucosides and total saponins of paeony, applied in medical preparations containing active ingredients, pill delivery, pharmaceutical formulations, etc., can solve problems such as complicated impurity removal process, and achieve easy solvent recovery, scientific and reasonable process, and operability. strong effect

Active Publication Date: 2013-01-23
傅宏征 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although ethyl acetate extraction is also used in the prior art, the process of removing impurities is very complicated

Method used

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  • Preparation method of total glucosides from Paeonia lactiflora Pall., and Paeonia lactiflora Pall. total glucoside preparation and use thereof
  • Preparation method of total glucosides from Paeonia lactiflora Pall., and Paeonia lactiflora Pall. total glucoside preparation and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Radix Paeoniae Alba is crushed into 10 kg of 12-mesh coarse particles, extracted with 75% ethanol water, extracted three times for 2 hours each time, combined the extracts, concentrated under reduced pressure, and the concentrated solution was subjected to resin column chromatography using an AB-8 resin column. Wash with water, then elute with 0.5-1% ammonia water, 70% methanol or ethanol, combine the 70% methanol or ethanol eluent and concentrate under reduced pressure to a specific gravity of 1.10-1.12; Extract three times, combine the extracts, recover under reduced pressure, concentrate to a relative density of about 110 (55 ° C thermal measurement), add 5% activated carbon to the concentrate, keep stirring for 30 minutes, decolorize and filter with suction, and concentrate the filtrate to a specific gravity of 1.20 (heat test at 65°C), vacuum drying or freeze-drying to obtain 380 g of total saponins of paeony powder. The total glycoside content is 81%.

Embodiment 2

[0041] Radix Paeoniae Alba was crushed into 10kg of 20-mesh coarse particles, extracted with water, extracted three times for 2 hours each time, combined the extracts, concentrated under reduced pressure, and the concentrated solution used D 101 The resin column is subjected to resin column chromatography, first washed with water, then eluted with 0.5-1% ammonia water, 70% methanol or ethanol, combined with 70% methanol or ethanol eluent and concentrated under reduced pressure to a specific gravity of 1.10-1.12 ; Extract three times with n-butanol (1: 1), combine the extracts, recover under reduced pressure, concentrate to a relative density of about 110 (55 ° C thermal measurement), add 5% activated carbon in the concentrated solution, keep stirring for 30 minutes, decolorize After the suction filtration is completed, the filtrate is concentrated to a specific gravity of 1.20 (65° C. thermal measurement), vacuum-dried or freeze-dried to obtain 415 g of total saponins of paeony...

Embodiment 3

[0043]Radix Paeoniae Alba is crushed into 10 kg of 15 mesh coarse particles, extracted with water, extracted three times for 2 hours each time, combined the extracts, concentrated under reduced pressure, and the concentrated solution was subjected to resin column chromatography using a HP-20 resin column, first washed with water, then Elute with 0.5-1% ammonia water, 70% methanol or ethanol, combine the 70% methanol or ethanol eluate and concentrate under reduced pressure to a specific gravity of 1.10-1.12; extract three times with n-butanol (1:1), The extracts were combined, recovered under reduced pressure, concentrated to a relative density of about 1.10 (thermally measured at 55° C.), 5% activated carbon was added to the concentrated solution, and kept stirring for 30 minutes. After decolorization, suction filtration was completed, and the filtrate was concentrated to a specific gravity of 1.20 (65 ℃ thermal measurement), vacuum drying or freeze-drying to obtain total sapon...

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Abstract

The invention relates to a preparation method of total glucosides from Paeonia lactiflora Pall., and a Paeonia lactiflora Pall. total glucoside preparation and use thereof. The method comprises: grinding, extracting, concentrating under reduced pressure, extracting, decolorizing, filtering, re-concentrating and drying. Column chromatography also can be used. The preparation method provided by theinvention greatly improves the content of total glucosides from Paeonia lactiflora Pall. to over 80 percent. The total glucosides from Paeonia lactiflora Pall., which is provided by the invention, can be used in the preparation of medicines for treating gynecological diseases, in particular the use in the preparation of medicines for treating dysmenorrheal, abnormal menstruation or endometriosis.The preparation method simplifies operation, adopts extraction solvent with low toxicity, allows for easy solvent recovery, is friendly to human beings and the environment and in accordance with environment protection requirements.

Description

technical field [0001] The invention relates to a process for extracting Chinese medicine components from Chinese medicinal materials, in particular to a method for extracting total saponins of paeony from the traditional Chinese medicine Radix Paeoniae Alba, its preparation of total saponins of paeony and its application. Background technique [0002] The traditional Chinese medicine Paeoniae Alba is the dried root of Paeoniae officinalis, which has the effects of calming the liver and relieving pain, nourishing blood and regulating menstruation, astringing yin and antiperspirant; it is used for headache, dizziness, hypochondriac pain, limb pain, blood deficiency and chlorosis, irregular menstruation, spontaneous sweating , Night sweats. Pharmacological studies have shown that Paeoniae Alba has antispasmodic, analgesic, immune regulation, and anti-inflammatory effects. The main active ingredients contained in Radix Paeoniae Alba are paeoniflorin, paeoniflorin, oxidized pae...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K36/71A61K9/08A61K9/10A61K9/16A61K9/20A61K9/48A61P15/00A61K125/00
Inventor 傅宏征李伟徐华
Owner 傅宏征
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