Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Loach protein antihypertensive peptide and preparation method thereof

A technology of antihypertensive peptides and antihypertensive peptides, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems that loach protein antihypertensive peptides have not been reported, and achieve good medicinal value and fecundity. Strong, steady-source effects

Active Publication Date: 2013-06-12
JIANGSU ACAD OF AGRI SCI
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research and application of loach are limited to the research on the antioxidant peptide of loach protein and the antibacterial and immunomodulatory activities of mucus polysaccharides, but there is no report on the antihypertensive peptide of loach protein

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Loach protein antihypertensive peptide and preparation method thereof
  • Loach protein antihypertensive peptide and preparation method thereof
  • Loach protein antihypertensive peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. The highly active ACE inhibitory peptide Leu-Glu-Tyr was isolated and purified from loach protein hydrolyzate.

[0032] (1) Enzymolysis of loach protein to prepare enzymatic solution

[0033] Grind loach meat into minced meat, add water to make a slurry, and then add 3‰ (w / w) bromelain (activity 3×10 5 U / g), adjust the pH value to 5.5, temperature 55°C, stir for 6 hours, inactivate the enzyme, centrifuge at 3000rpm for 20min, and the supernatant is the enzymolysis solution.

[0034] (2) Separation and purification of hypotensive peptides from enzymatic hydrolyzate

[0035] The enzymatic solution was decolorized with 2% (w / v) activated carbon for 20min, then passed through a 2500Da ultrafiltration membrane ultrafiltration, and the ultrafiltrate with an ultrafiltration of less than 2500Da was passed through a Sephadex G-15 column (1.6 × 100cm, Amersham Pharmacia Biotech , Piscataway, NJ, USA), flow rate 1.0mL / min, separated to obtain 5 peaks, automatic fracti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a loach protein antihypertensive peptide and a preparation method thereof, belonging to the technical field of biology. The antihypertensive short peptide separated and purified from an enzymolysis product of loach protein has high activity in inhibiting angiotensin converting enzyme (ACE). The preparation method provided by the invention comprises the following steps of: carrying out enzymolysis on the loach protein to prepare an enzymolysis liquid; separating and purifying the antihypertensive peptide from the enzymolysis liquid; and sequencing the antihypertensive peptide. The invention combines a modern enzyme engineering technique and a peptide purifying and identifying technique, deep development and utilization on the loach protein are utilized, and a novel-sequence short peptide Leu-Glue-Tyr with high ACE inhibiting activity is found; and the novel loach protein antihypertensive peptide provided by the invention has high ACE inhibiting activity and has an IC50 value of 0.103 mg / mL.

Description

1. Technical field [0001] The invention relates to an antihypertensive peptide with a new amino acid sequence isolated and purified from an enzymatic hydrolyzate of loach meat and its preparation method and application, which applies modern biotechnology to the deep processing of freshwater aquatic products, and belongs to the technical field of deep processing of freshwater aquatic products . 2. Background technology [0002] Most of the hydrolyzed products obtained by enzymatic hydrolysis of food-derived proteins are short peptides composed of several amino acid residues. These short peptides are absorbed into the circulatory system in a complete form and used by tissues. They have many physiological activities: anti-oxidation, improving Immunity, antibacterial and anti-inflammatory, promotion of passive transport of trace elements and storage in the body, antihypertensive activity, etc. [0003] Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptide carboxyl ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/083A61P9/12C12P21/06C07K1/36C07K1/34C07K1/16
Inventor 李莹周剑忠曾晓雄黄开红王英马艳弘张丽霞
Owner JIANGSU ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com