Normal pulse stripping method for detecting content of vitamin in blood samples

A vitamin and blood sample technology, applied in the direction of electrochemical variables of materials, etc., can solve the problems of high price, long detection time, and complicated detection methods.

Active Publication Date: 2011-09-21
天津市兰标电子科技发展有限公司
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Problems solved by technology

Due to high-performance liquid chromatography, fluorescence analyzer and other instruments are expensive, and require specialize...

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  • Normal pulse stripping method for detecting content of vitamin in blood samples
  • Normal pulse stripping method for detecting content of vitamin in blood samples

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Embodiment 1

[0028] Conventional pulse dissolution method for detecting vitamin content in blood samples: take a certain amount of sample to be tested and mix it with a certain amount of self-made vitamin sample treatment solution, and use conventional pulse dissolution method to make the vitamins in the sample to be tested undergo oxidation (reduction) on the self-made sensor probe ) React to generate a current signal. By comparing the current signal values ​​of standard samples of vitamins with different concentrations, a standard curve is developed, and the content of vitamins in the sample to be tested is obtained according to the value of the current signal generated by the sample to be tested.

[0029] The specific implementation process of detecting the content of vitamin B2 in blood samples:

[0030] 1. Prepare vitamin K3 sample treatment solution: Vitamin K3 sample treatment solution is composed of ammonium chloride, hydrochloric acid, and tetramethylammonium chloride, and the additio...

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Abstract

The invention relates to a normal pulse stripping method for detecting the content of vitamin in blood samples, belonging to the technical field of vitamin analysis and detection. The method is characterized by comprising the following steps: taking blood samples to be detected, mixing the blood samples to be detected with a vitamin sample treatment solution, and detecting current signals generated when the blood samples are subjected to oxidation reduction reaction on a sensor probe of a vitamin detector by using the normal pulse stripping method; and making a standard curve through comparing the current signal values of standard samples with different vitamin concentrations, then obtaining the content of the vitamin in the blood samples to be detected according to the current signal values generated by the samples to be detected. The method provided by the invention has the advantages of high sensitivity, good accuracy, simplicity and convenience in operation, quickness, wide application range, suitability for being used in medical and health departments so as to carry out analysis and detection on vitamins A, B1, B2, B6, B9, B12, C, D, E, K1 and K3 in blood samples, and the like; and the method can be used for rapidly detecting the content of the vitamin in the blood samples.

Description

Technical field [0001] The invention belongs to the technical field of vitamin analysis and detection, and particularly relates to a conventional pulse dissolution method for detecting the vitamin content of a blood sample. Background technique [0002] Vitamins are a type of trace organic matter that the body must ingest from food to maintain normal physiological functions. It plays an important role in regulating material metabolism and maintaining physiological functions. Long-term lack or excessive intake of certain vitamins will cause corresponding diseases. If vitamin A is lacking, night blindness, dry eye disease and dry skin may occur; lack of vitamin D may cause rickets; lack of vitamin B12 may cause pernicious anemia; excessive intake of vitamin E will destroy blood clotting function and increase the possibility of bleeding; intake of more than 1000 Milligrams of vitamin C can affect the detoxification function of the kidneys. [0003] At present, the instruments and de...

Claims

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Application Information

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IPC IPC(8): G01N27/26
Inventor 范清杰
Owner 天津市兰标电子科技发展有限公司
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