Solid forming method of active cell-hydrogel organ structure

Abstract

The invention provides a solid forming method of an active cell-hydrogel organ structure. The method comprises the following steps: firstly designing a hydrogel unit model according to natural organs; manufacturing a physical prototype of the unit by utilizing a micro-manufacturing technology, and then filling the physical prototype to obtain a negative structure; treating the surface of the negative structure, and then filling a negative die with a biological material-cell mixed solution; gelating the mixed solution in a cross-linking manner to obtain an active cell-hydrogel unit with a biomimetic blood flow channel; locating and superposing layer by layer and then performing secondary cross-linking to form the active cell-hydrogel organ structure; and finally culturing in vitro or implanting in vivo to construct a pre-vascularized active organ precursor. In the method, by assembling the two-dimensional active cell-hydrogel structure unit in a superposition manner, a complicated three-dimensional (3D) organ micro-fluidic channel system is constructed and cells are uniformly and sequentially seeded in a 3D space.

Description

technical field [0001] The invention relates to a forming method of an artificial active organoid structure, in particular to a three-dimensional forming method of an active cell-hydrogel organoid structure. Background technique [0002] In the 21st century, the in vitro manufacturing of human tissues and organs through the combination of manufacturing technology and biomedicine will bring fundamental changes to clinical treatment methods, thus benefiting thousands of patients. At present, artificial skin, cartilage, bone, bladder, etc. have entered the clinical application or experimental stage, but the in vitro manufacturing of important organs such as liver, kidney, lung, and heart has not made breakthroughs so far. The difficulty lies in the use of soft biomaterials to biomimetically construct a three-dimensional microstructure system and cell growth environment similar to natural organs in vitro. The traditional tissue engineering model of first forming the scaffold st...

AI Technical Summary

Problems solved by technology

The traditional tissue engineering model of first forming the scaffold structure and then planting cells cannot achieve high-density uniform planting of cells in the scaffold and orderly distribution of different cells, and more importantly, it cannot quickly achieve vascularization, so that cells inside the scaffold often due to Slow growth or death due to lack of adequate nutrient supply

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