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Enterococcus faecium and method for producing equol by using Enterococcus faecium and application of Enterococcus faecium

A technology of Enterococcus faecium and equol is applied in the field of Enterococcus faecium and its production of equol, and can solve the problems such as the inability to produce equol

Active Publication Date: 2011-09-28
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these bacteria cannot produce equol in pure culture

Method used

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  • Enterococcus faecium and method for producing equol by using Enterococcus faecium and application of Enterococcus faecium
  • Enterococcus faecium and method for producing equol by using Enterococcus faecium and application of Enterococcus faecium
  • Enterococcus faecium and method for producing equol by using Enterococcus faecium and application of Enterococcus faecium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation of Enterococcus faecium BY1

[0033] The preparation method of Enterococcus faecium BY1 is as follows:

[0034] 1. Collection of samples:

[0035] Take 1 g of fresh feces from infants, put them in 9 g of normal saline with a mass fraction of 0.85%, store them in a refrigerator at 4°C, and inoculate them within 12 hours.

[0036] 2. Gradient dilution of samples and screening of equol-producing strains:

[0037] (1) Single colony isolation culture

[0038] The previously prepared samples were serially diluted with sterile saline, diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 、10 -7 times, select 10 -5 、10 -6 、10 -7 The three gradients were inoculated into the pre-prepared Hungate anaerobic tube containing BHI solid medium, flushed with high-purity nitrogen with a nitrogen blower, and cultured anaerobically at 37°C for 48 hours.

[0039] (2) Screening of single colony with equol-producing function

[0040] Inoculate the single bacteriu...

Embodiment 2

[0051] Embodiment 2 Enterococcus faecium BY1 (Enterococcus faecium BY1) produces the method for equol

[0052] The method proceeds as follows:

[0053] (1) Expansion of Enterococcus faecium BY1 strain

[0054] Place the strain of Enterococcus faecium BY1 in a 37°C incubator for activation, inoculate 1 / 20 of the inoculum into the sterilized BHI medium in the Hungate anaerobic tube, and fill the nitrogen blower with high-purity Nitrogen, cultivated at 37°C for 48 hours to obtain the expansion culture fermentation liquid;

[0055] (2) Bacterial fermentation and metabolism to produce isoflavone compositions containing equol

[0056] Prepare the isoflavone-BHI medium, wherein the daidzein content is 14% of the total mass of isoflavones, the content of genistin is 57% of the total mass of isoflavones, the pH is adjusted to 6.6, and the nitrogen blowing instrument is filled with high-purity nitrogen gas for sterilization , sterilize the syringe to inoculate the expansion culture b...

Embodiment 3

[0059] Example 3 Manufacture of equol-containing fermented soybean milk

[0060] To prepare the equol-containing composition in the form of fermented soy milk, weigh and mix the ingredients according to the following list:

[0061]

[0062] The fermentation culture of soybean milk powder is prepared by the following method, adding 15g of commercially available soybean milk powder into 100mL of warm water, and adding Enterococcus faecium BY1 cultured for 48 hours to it, and culturing at 37°C for 12-14 days to obtain Fermentation culture of soybean milk powder containing equol.

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Abstract

The invention discloses Enterococcus faecium BY1, a method for producing equol by using the Enterococcus faecium and application of the Enterococcus faecium, and belongs to the field of biochemical industry. The bacteria were collected in China General Microbiological Culture Collection Center on March 28, 2011, and the collection number is CGMCC NO.4707. The Enterococcus faecium has the capacity of transforming a daidzin part in isoflavone into the equol, and can be used for preparing food containing the equol.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and specifically relates to an Enterococcus faecium and a method and application thereof for producing equol. Background technique [0002] Isoflavones belong to polyphenolic compounds, so they are easier to combine with glucose to form β-glucosidic bond coupling, which is also the main form of isoflavones in soy products. However, the conjugated isoflavones are not biologically active and cannot be absorbed by the human body. Under normal circumstances, the β-glucose enzyme in the stomach must first metabolize daidzein into the form of daidzein. Uptake and subsequent utilization are critical. [0003] Equol (equol) is a derivative of isoflavone, which is the product of human intestinal bacteria degrading daidzein. One of the currently recognized metabolic pathways of equol can be described as (Juliana, 2003): after the human body ingests daidzein, etc., the coupled isoflavones are hydrolyze...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/06A23L1/29C12R1/46A23L33/00
Inventor 程永强殷丽君刘鹏冯淑环陈明海王盼
Owner CHINA AGRI UNIV
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