Cyclic square wave voltammetry for detecting vitamin content in blood sample

A square wave voltammetry and vitamin technology, applied in the direction of electrochemical variables of materials, etc., can solve the problems of complicated detection methods, long detection time, difficult to popularize, etc., and achieve the effects of good accuracy, simple operation and high sensitivity

Active Publication Date: 2011-10-05
天津市兰标电子科技发展有限公司
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Problems solved by technology

Due to high-performance liquid chromatography, fluorescence analyzer and other instruments are expensive, and require specialized t

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  • Cyclic square wave voltammetry for detecting vitamin content in blood sample
  • Cyclic square wave voltammetry for detecting vitamin content in blood sample

Examples

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Embodiment 1

[0028] Cyclic square wave voltammetry to detect vitamin content in blood samples: Take a certain amount of sample to be tested and mix a certain amount of homemade vitamin sample treatment solution, and use cyclic square wave voltammetry to make the vitamins in the sample to be tested on the self-made sensor probe An oxidation (reduction) reaction occurs, generating a current signal. By comparing the current signal values ​​of vitamin standard samples with different concentrations, a standard curve is developed, and the vitamin content in the sample to be tested is obtained according to the current signal value generated by the sample to be tested.

[0029] The specific implementation process of detecting vitamin D content in blood samples:

[0030] 1. Prepare vitamin D sample treatment solution: Vitamin D sample treatment solution is composed of perchlorate, strong acid, and dimethylformamide; perchlorate is sodium perchlorate and potassium perchlorate, and the amount of per...

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Abstract

The invention relates to cyclic square wave voltammetry for detecting vitamin content in a blood sample. The invention belongs to the technical field of vitamin analysis and detection. The cyclic square wave voltammetry for detecting vitamin content in a blood sample is characterized by mixing a blood sample to be measured with a vitamin sample treatment fluid, detecting current signals generated by the sample in an oxidation reduction reaction on the sensor probe of a vitamin detector, formulating a standard curve based on a comparison of current signal values of vitamin standard samples with different concentration, and obtaining the vitamin content in the blood sample to be measured based on current signal values generated by the sample. The invention has the advantages of high sensitivity, good accuracy, simple operation, a high speed and a wide application scope; therefore, the invention is applicable to medical departments, and can be used for the analysis and detection of vitamin A, B1, B2, B6, B9, B12, C, D, E, K1 and K3 and for a rapid detection of vitamin content in a blood sample.

Description

technical field [0001] The invention belongs to the technical field of vitamin analysis and detection, in particular to a circulating square wave voltammetry method for detecting vitamin content in blood samples. Background technique [0002] Vitamins are a class of trace organic substances that the body must ingest from food in order to maintain normal physiological functions, and play an important role in regulating substance metabolism and maintaining physiological functions. Long-term lack or excessive intake of certain vitamins can lead to corresponding diseases. For example, night blindness, dry eyes, and dry skin can occur due to vitamin A deficiency; rickets can occur due to vitamin D deficiency; pernicious anemia can occur due to vitamin B12 deficiency; excessive intake of vitamin E can damage blood coagulation and increase the possibility of bleeding; intake of more than 1000 mg of vitamin C can affect the detoxification function of the kidneys. [0003] At prese...

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Application Information

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IPC IPC(8): G01N27/48
Inventor 范清杰
Owner 天津市兰标电子科技发展有限公司
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