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Culture medium for culturing lilium pumilum tissues

A tissue culture, lily lily technology, applied in the field of plant biology, can solve the problems of disease infection quality, degradation, limited reproduction number, etc.

Active Publication Date: 2013-04-17
INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificial propagation of Lily lily still mainly adopts the method of separate planting small bulbs, sowing and scale cuttings, the number of reproduction is limited and it is easy to cause disease infection and quality degradation
However, there are few studies on tissue culture propagation that can be used to solve the problem of degeneration and rejuvenation. Therefore, it has become one of the urgent tasks for scientific researchers to carry out in-depth research on tissue culture of Lily lily.

Method used

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  • Culture medium for culturing lilium pumilum tissues
  • Culture medium for culturing lilium pumilum tissues
  • Culture medium for culturing lilium pumilum tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The material of embodiment 1. Lilium tenifolia and screening statistical method

[0017] The lily lily used in the experiment was collected from Daqingshan, a suburb of Hohhot, and the collected bulbs were planted in the experimental nursery of our company. Samples were taken according to the progress of the experiment, at different periods, and supplemented at any time. The explants are the bulbs of Lilium microphylla.

[0018] Using MS as the basic medium, using 3.5g / L carrageenan as the solidifying agent, 3% edible sugar instead of sucrose as the carbon source, tap water instead of distilled water, and adding different ratios of cytokinin and auxin to the culture medium filter.

Embodiment 2

[0019] Example 2. Screening of the optimal medium for each stage of Lilium microphylla tissue culture

[0020] 1. Selection of induction differentiation medium

[0021] Set the concentration of 6-BA to five concentration gradients of 0.2mg / L, 0.5mg / L, 1.0mg / L, and 2.0mg / L, and set the concentration of NAA to 0.1mg / L, 0.2mg / L, 0.3mg / L, Four concentration gradients of 0.5mg / L were used in different combinations. When inoculating, each bottle was inoculated with a piece of bulb, and the occurrence of adventitious buds of the scale on different media was observed.

[0022] It can be seen from Table 1 that all seven hormone combinations can induce lily scales to differentiate into small bulbs, but the differentiation rates of each combination are different. No. 1 MS+6-BA 0.1mg / L+NAA 0.1mg / L is the medium for lily scales It is the best induction medium, not only has a high induction rate, but also has a large number of new shoots per explant and the highest differentiation rate, wh...

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Abstract

The invention relates to a culture medium for culturing lilium pumilum tissues. The best culture mediums for culturing the lilium pumilum tissues at different stages are screened; the proportion of various hormone in the culture medium is determined; and the culture mediums prepared according to the formula can meet the nutrient requirement and growth and development of the lilium pumilum at eachstage. The culture mediums comprise a first generation induction culture mediums consisting of MS, 1.0 mg / L of 6-BA and 0.1 mg / L of NAA, a subgeneration proliferation culture medium consisting of MS,0.2 mg / L of 6-BA and 0.01 mg / L of NAA, a rooting culture medium consisting of 1 / 2 MS and 0.5 mg / L of IBA and a capitulum culture medium consisting of 1 / 2 MS and 0.5 mg / L of IBA and 2 percent of sugar.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a culture medium of various stages in the tissue culture of Lilium pumilum. The present invention screens out the optimal culture medium for different periods in the tissue culture of Lilium tenifolia, determines the ratio of each hormone in the medium, and the medium formulated according to the formula of the present invention can satisfy the nutrition of Lilium tenifolia in various periods needs and growth. Background technique [0002] Lilium pumilum, also known as Shandan flower, is a perennial bulbous flower of the genus Lilium in the family Liliaceae. Lily lily grows in arid and semi-arid areas. It has tenacious vitality and can survive the winter in the wild. It can also grow normally in the case of little rain and no irrigation. It can meet the requirements of greening in terms of performance and appearance. It can be used and promoted in drought-resistant greening la...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 王召明梁德霖姚裕琪
Owner INNER MONGOLIA M GRASS ECOLOGY & ENVIROMENT GRP CO LTD
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