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Method for identifying gray mold resistance of gerbera

A technology for identification of resistance and botrytis, applied in the direction of microbial-based methods, biochemical equipment and methods, and microbial measurement/inspection, can solve the problems of plant infection and disease, long identification period, and the result is vulnerable to environmental conditions To achieve the effect of promoting the breeding and accurate identification of gerbera botrytis resistance

Inactive Publication Date: 2011-10-19
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the deficiencies and deficiencies that the field live plant inoculation method of the prior art has the defects and deficiencies of test plant infection, long identification period, and easily affected by environmental conditions to the identification of gerbera gray mold resistance. The purpose is to provide a simple, rapid, accurate and large-scale method for screening and identifying gerbera botrytis resistance resources, providing support for further cross breeding or molecular marker assisted breeding

Method used

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  • Method for identifying gray mold resistance of gerbera
  • Method for identifying gray mold resistance of gerbera
  • Method for identifying gray mold resistance of gerbera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1 The embodiment using petals as inoculation material

[0041] (1) Plant material

[0042] The gerbera cut-flower cultivars tested with different resistance to Botrytis cinerea were: Macy, Kaliki, Risto, Serena, CK100, 3142, 5544, a total of 7 genotypes, provided by Dutch gerbera breeding company Florist and Shreurs, and fresh petals were collected as Inoculation material;

[0043] (2) Botrytis cinerea Botrytis cinerea Culture and preparation of conidia suspension

[0044] Botrytis cinerea was cultivated with potato dextrose agar medium (abbreviation: PDA medium) Botrytis cinerea , Botrytis cinerea ( Botrytis cinerea) inoculated in the center of a 10cm-diameter petri dish with potato dextrose agar medium on a superbacteria workbench, and cultivated under continuous light for 16 hours at a temperature of 20°C to 22°C and a light intensity of 1800lx. After 3 days of cultivation, it was transferred to dark conditions for cultivation After 7 days, after t...

Embodiment 2

[0063] Embodiment 2 is the embodiment of inoculation material with complete young leaves

[0064] (1) Plant material

[0065] The gerbera cut flower cultivar and source for testing are the same as in Example 1, and the fresh complete young leaves are collected as inoculation material;

[0066] (2) Botrytis cinerea Botrytis cinerea Culture and preparation of conidia suspension

[0067] With embodiment 1.

[0068] (3) Preparation of inoculum

[0069] With embodiment 1.

[0070] (4) Inoculation

[0071] Inoculate 2 μl of the inoculum solution containing different concentrations of conidia and PD prepared in step (3) onto the young leaves described in step (1), and keep the young leaves in a plastic film, with a relative humidity of 90% to 95 %, under the condition of light intensity of 1800lx and temperature of 20℃~22℃, the disease development was observed 24 hours after inoculation, and the disease resistance of the tested materials was judged according to the disease dev...

Embodiment 3

[0086] Example 3 Test of suitable inoculation material

[0087] Except that the inoculation material described in Example 1 was tested, Example 2 also tested the gerbera leaf block and stem section, and the test method was carried out using the treatment method in Example 1.

[0088] (1) Plant material

[0089] The gerbera cut-flower cultivars tested with different resistance to Botrytis cinerea were: Macy, Kaliki, Risto, Serena, CK100, 3142, 5544, a total of 7 genotypes, provided by Dutch gerbera breeding companies Florist and Shreurs, which were fresh Collected gerbera leaf pieces and stem sections are used as inoculation materials. The leaf pieces have three types of diameters: 1.5cm, 2.5cm, and 3.5cm, and the length of the stem section is 8cm.

[0090] (2) Botrytis cinerea Botrytis cinerea Culture and preparation of conidia suspension

[0091] Botrytis cinerea was cultivated with potato dextrose agar medium (abbreviation: PDA medium) Botrytis cinerea , Inoculate the ...

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Abstract

The invention discloses a method for identifying gray mold resistance of gerbera, belonging to the technical field of plant disease resistance identification. The method comprises the following steps: (1) selection of proper inoculation material: selecting freshly collected and integrated young leaves and petals as the inoculation material; (2) culture of inoculation material and preparation of conidia suspension; (3) preparation of inoculation liquid: before inoculation, diluting the conidia stock solution into an inoculation liquid containing 3 wt% of potato glucose and 1*10<6> / ml conidia, or into an inoculation liquid containing 6wt% of potato glucose and 3*10<5> / ml conidia; and (4) inoculation. The method disclosed by the invention is carried out under manually controlled stable conditions, and achieves the goal of identifying the gray mold resistance of gerbera in a simple, quick and accurate way; and the accuracy of the identification result is high.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance identification, and in particular relates to a method for in vitro resistance identification of gerbera gray mold. Background technique [0002] Gerbera ( Gerbera spp .) is one of the five most important cut flowers in the world, and the application of potted flowers is becoming more and more popular, which has important economic value. Botrytis ( Botrytis cinerea ) is an air-borne saprophytic fungus with a wide suitable temperature range (4°C-25°C) and infects more than 200 kinds of hosts. Especially in the post-harvest storage and transportation of cut gerbera flowers, environmental conditions such as high humidity and large temperature fluctuations produce condensation, which provides good conditions for the growth and reproduction of Botrytis cinerea, and soft rot caused by Botrytis cinerea has become a One of the limiting factors for post-harvest quality maintenance of cu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/645
Inventor 吴红芝吴景芝吴兴恩陈溪都婷
Owner YUNNAN AGRICULTURAL UNIVERSITY
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