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Preparation method of macroporous cross-linked sodium alginate gel beads

A technology of sodium alginate, gel ball, applied in chemical instruments and methods, other chemical processes, alkali metal compounds, etc.

Inactive Publication Date: 2011-10-26
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the technology of preparing macroporous gel spheres with good protein adsorption capacity by cross-linking using its own carboxyl groups

Method used

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  • Preparation method of macroporous cross-linked sodium alginate gel beads
  • Preparation method of macroporous cross-linked sodium alginate gel beads
  • Preparation method of macroporous cross-linked sodium alginate gel beads

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Step 1: Dissolve 8g of sodium alginate and 32g of sodium sulfate in 400mL of deionized water.

[0025] The second step: drop the above solution into 500ml of 5% calcium chloride solution through a 0.5mm pinhole, and stir the calcium chloride solution at the same time. After the dropwise addition was complete, it was stirred for 8 hours.

[0026] The third step: filter the alginic acid gel prepared in the second step with gauze, and wash it with ethanol.

[0027] Step 4: Measure 50mL of the above-mentioned alginic acid gel balls into a 250mL three-necked flask, add 50mL of epichlorohydrin and 5g of NaOH, stir, raise the temperature to 60°C, and react for 8 hours. Cool to room temperature, filter out the cross-linked alginate gel balls with gauze, and wash with ethanol.

[0028] Step 5: Place the cross-linked alginic acid gel balls prepared above in 100 mL of 0.1M EDTA solution for 4 hours to dissolve the calcium sulfate in the cross-linked alginic acid gel balls, and r...

Embodiment 2

[0037] Step 1: Dissolve 8g of sodium alginate and 16g of sodium sulfate in 400mL of deionized water.

[0038] The second step: drop the above solution into 500ml of 5% calcium chloride solution through a 0.5mm pinhole, and stir the calcium chloride solution at the same time. After the dropwise addition was complete, it was stirred for 8 hours.

[0039] The third step: filter the alginic acid gel prepared in the second step with gauze, and wash it with ethanol.

[0040] Step 4: Measure 50mL of the above-mentioned alginic acid gel balls into a 250mL three-necked flask, add 50mL of epichlorohydrin and 5g of NaOH, stir, raise the temperature to 60°C, and react for 8 hours. Cool to room temperature, filter out the cross-linked alginate gel balls with gauze, and wash with ethanol.

[0041] Step 5: Place the cross-linked alginic acid gel balls prepared above in 100 mL of 0.1M EDTA solution for 4 hours to dissolve the calcium sulfate in the cross-linked alginic acid gel balls, and r...

Embodiment 3

[0047] Step 1: Weigh 8g of sodium alginate and dissolve it in 400mL of deionized water.

[0048] The second step: drop the above solution into 500ml of 5% calcium chloride solution through a 0.5mm pinhole, and stir the calcium chloride solution at the same time. After the dropwise addition was complete, it was stirred for 8 hours.

[0049]The third step: filter the alginic acid gel prepared in the second step with gauze, and wash it with ethanol.

[0050] Step 4: Measure 50mL of the above-mentioned alginic acid gel balls into a 250mL three-necked flask, add 50mL of epichlorohydrin and 5g of NaOH, stir, raise the temperature to 60°C, and react for 8 hours. Cool to room temperature, filter out the cross-linked alginate gel balls with gauze, and wash with ethanol.

[0051] Step 5: Place the cross-linked alginic acid gel balls prepared above in 100 mL of 0.1M EDTA solution for 4 hours to dissolve the calcium sulfate in the cross-linked alginic acid gel balls, and repeat the oper...

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Abstract

The invention discloses a preparation method of macroporous cross-linked sodium alginate gel beads. According to the method, an aqueous solution of a mixture of sodium alginate and sodium sulfate is dropped into calcium chloride solution; while sodium alginate forms gel beads, sodium sulfate forms calcium sulfate deposition; sodium alginate gel beads are cleaned with ethanol, and are subject to a reaction with epichlorohydrin, such that cross-linked sodium alginate gel is formed; calcium sulfate within cross-linked sodium alginate gel is solved; the obtained material is cleaned, such that macroporous cross-linked sodium alginate gel beads are obtained. The macroporous cross-linked sodium alginate gel beads prepared by the method has good bovine serum albumin absorbance, and has good application prospect in the respects of biologically-active substance extraction purification.

Description

(1).Technical field [0001] The invention relates to a preparation method of sodium alginate gel, in particular to a preparation method of macroporous cross-linked sodium alginate gel balls. (2). Background technology [0002] In recent years, biotechnology has developed very rapidly. Using biotechnology such as genetic engineering, protein engineering, and fermentation engineering, it has been possible to design, manufacture, and produce a variety of proteins that people urgently need. Like the production process of other biological products, the production process of protein is generally divided into upstream, middle and downstream processes. Downstream technology occupies a large proportion of the cost of biotechnology products, and the cost of the entire separation and purification process accounts for about 60% to 80% of the cost, which is the key to the industrialization of biotechnology. Ion exchange adsorbent is currently the most widely used separation medium in pr...

Claims

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Application Information

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IPC IPC(8): C08J9/26C08J3/24C08J3/14C08J3/075C08L5/04B01J20/24B01J20/28B01J20/30
Inventor 张为灿卢雪梅
Owner SHANDONG UNIV
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