Preparation method of composite extract for fermentation type tobacco
A fermented and extract technology, which is applied in the field of preparation of fermented tobacco compound extract, can solve the problems of application limitations and achieve the effects of increasing sweetness, improving taste and reducing irritation
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Embodiment 1
[0036] 1) Culture medium preparation
[0037] a Raw materials pre-treatment: Broken Rosa roxburghii and sweet and sour horn into large granules, and mixed 20 parts, 20 parts and 60 parts by weight respectively of Rosa rufica, Lycium barbarum and sweet and sour horn, and mixed raw materials and water according to The mass ratio is 1:8, reflux extraction for 3 hours, filtering with a 0.8 μm microporous membrane, concentrating, and concentrating to a relative density of 1.07 to obtain a composite extract;
[0038] b. Culture medium preparation: add 0.5% glucose and 0.5% sodium chloride of the compound extract mass to the prepared compound extract to prepare the culture medium;
[0039] 2) Vaccination
[0040] Sterilize the medium at 121°C for 20 minutes, and inoculate the aroma-producing active dry yeast into the medium under aseptic conditions, the inoculation ratio is 0.5% of the mass of the medium;
[0041] 3) fermentation
[0042] Ferment for 3 days at a pH value of 4.0, a...
Embodiment 2
[0046] 1) Culture medium preparation
[0047] a Raw materials pre-treatment: Broken Rosa roxburghii and sweet and sour horn into large granules, and mixed 10 parts, 15 parts and 50 parts by weight respectively of Rosa rufica, Lycium barbarum and sweet and sour horn, and mixed raw materials and water according to The mass ratio is 1:5, reflux extraction for 2 hours, filter with a 0.8 μm microporous membrane, concentrate, and concentrate to a relative density of 1.03 to obtain a composite extract;
[0048] b. Culture medium preparation: add 0.1% glucose and 0.1% sodium chloride of the compound extract mass to the prepared compound extract to prepare the medium;
[0049] 2) Vaccination
[0050] Sterilize the medium at 121°C for 30 minutes, and inoculate the aroma-producing active dry yeast into the medium under aseptic conditions, and the inoculation ratio is 0.01% of the mass of the medium;
[0051] 3) fermentation
[0052] Ferment for 9 days at a pH value of 2.5, a stirring sp...
Embodiment 3
[0056] 1) Culture medium preparation
[0057] a Raw materials pre-treatment: Fragrance rosae and sweet and sour horn are broken into large granules, and Rosa rosa, wolfberry and sweet and sour horn are mixed in parts by weight of 25 parts, 25 parts and 75 parts respectively, and the raw materials after mixing are mixed with water according to The mass ratio is 1:15, reflux and extract for 5 hours, filter with a 0.8 μm microporous membrane, concentrate, and concentrate to a relative density of 1.12 to obtain a composite extract;
[0058] b. Culture medium preparation: add 5% glucose and 0.9% sodium chloride of the compound extract mass to the prepared compound extract to prepare the culture medium;
[0059] 2) Vaccination
[0060] Sterilize the medium at 121°C for 15 minutes, and inoculate the aroma-producing active dry yeast into the medium under aseptic conditions, the inoculation ratio is 2% of the mass of the medium;
[0061] 3) fermentation
[0062] Ferment for 2 days a...
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