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A technology of soybean oil and extraction method, which is applied in the direction of oil/fat production and fat production, and can solve the problems of low oil yield and the like
Inactive Publication Date: 2011-11-02
NORTHEAST AGRICULTURAL UNIVERSITY
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[0004] The purpose of the present invention is to study and determine a plurality of process parameters in the pretreatment method of vacuum extrusion puffing and micro-grinding, aiming at the problem of low oil yield in the process of enzymatic oil extraction by water, to study a A method for extracting soybean oil to achieve the purpose of improving the yield of soybean oil
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Embodiment 1
[0024] Example 1: Screening experiment of optimal parameters for vacuum extrusion puffing of peeled soybean flakes
[0025] 1 Materials and methods
[0026] 1.1 Materials and reagents
[0027] Dehulled soybean flakes Harbin Jiusan Oil Group
[0028] protex-6L alkaline endoprotease Danish novo company
[0029] 1.2 Main instruments and equipment
[0030] pHS-25 acidity meter Shanghai Weiye Instrument Factory
[0037] Hammer Mill China Tianjin Test Instrument Co., Ltd.
[0038] Soxhlet Extractor Tianjin Glas...
Embodiment 2
[0073] Example 2: Screening experiment for optimal parameters of vacuum puffed soybean micronization
[0074] Based on the best vacuum extrusion technique for extracting soybean oil determined in Example 1, a single-factor micro-sized pulverization test was carried out to determine the micro-sized process parameters (ball milling time, ball milling speed, material filling rate, medium filling rate) scope. Taking the total oil extraction rate as the investigation index, a response surface design experiment with 4 factors and 5 levels was carried out.
[0075] 1 Materials and methods
[0076] 1.1 Materials and reagents
[0077] Dehulled soybean flakes Harbin Jiusan Oil Group
[0078] protex-6L alkaline endoprotease Danish novo company
[0079] 1.2 Main instruments and equipment
[0080] pHS-25 acidity meter Shanghai Weiye Instrument Factory
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Abstract
The invention discloses an extraction method of soybean oil and belongs to the technical field of extraction and processing of plant oil. The extraction method comprises the following steps that: dehulled soybean pieces as a raw material are pretreated by a vacuum extrusion-expansion process and a superfine ball-grinding process to form a soybean product; the soybean product is mixed with water; a protease is added into the mixture obtained the previous step and undergoes an enzymolysis reaction with the mixture; and the reaction products are subjected to a centrifugal separation process and soybean oil is obtained. Parameters of the vacuum extrusion-expansion process comprise that a mould aperture size is in a range of 14 to 26 mm; a screw speed is in a range of 70 to 160 rotations per minute; a sleeve temperature is in a range of 75 to 120 DEG C; and a vacuum degree is in a range of -0.03 to -0.07 MPa. Parameters of the superfine ball-grinding process comprise that a ball-grinding time is in a range of 20 to 100 min; a ball-grinding speed is in a range of 100 to 220 rotations per minute, a material filling rate is in a range of 10 to 30%; and a medium filling rate is in a range of 10 to 30%. The extraction method has the advantages of simple used equipment, safe operation, good soybean oil product properties of no solvent residue and high nutritional values, and high total oil extraction rate reaching to about 97.48%.
Description
technical field [0001] The invention belongs to the field of extraction and processing of vegetable oils, in particular to a method for extracting soybean oils by using an aqueous enzymatic method. Background technique [0002] Aqueous enzymatic extraction technology is the most ideal method to obtain oil and protein at the same time from oil, but it is also a difficult process to study, especially for oil with low oil content (the most typical is soybean), the oil yield is low, And the technical problems such as difficult demulsification and separation of the emulsion formed by protein and oil after enzymatic hydrolysis. [0003] The method of extracting vegetable oil with water as a solvent is applied to oil plants with low oil content like soybean, the effect is poor, and the oil extraction rate is low. The protein contained in soybean is not only large in quantity, but also excellent in quality. It is a high-quality plant protein. Therefore, it is of great significance ...
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