Dark current detection method

A detection method and dark current technology, applied in the direction of measuring current/voltage, measuring device, measuring electrical variables, etc., can solve problems such as long time, achieve the effects of improving accuracy, easy implementation, and simple structure

Inactive Publication Date: 2011-11-09
上海永创生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of relatively long time in the existing method, to provide a method for real-time detection of dark current, which improves the detection speed,

Method used

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Embodiment Construction

[0013] The embodiment of the present invention will be specifically described below by taking a microplate reader as an example.

[0014] The schematic diagram of the optical system of the microplate reader is shown in Figure 1 shown. The light emitted by the light source lamp 1 passes through the filter in the light passage hole of the filter wheel 2, and then transmits the optical signal to the eight photocells 5 test channels through the optical fiber 3 for testing. And output the corresponding electrical signal for subsequent calculation of absorbance.

[0015] For each measurement, turn the filter wheel to the filter position of the specified wavelength, before the bracket moves, the photocell is not blocked, read the AD conversion value A0 of the light source passing through the air, and move the opaque frame of the bracket 4 to the photocell Right above, block the light source, detect the dark current reading A1, use the bracket to move the 96-well plate equipped with...

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Abstract

The invention discloses a dark current detection method which comprises: a light source, an optical filter wheel, a fiber, photocells, a bracket, a stepping motor, a hold-in range, a photoelectric switch. The photocells are used for receiving optical signals and outputting corresponding electrical signals. The optical filter wheel is equipped with optical filters. The bracket is provided with an opaque framework. In the invention, the photocells can be shielded by the opaque framework of the bracket so that the dark current can be measured without cutting off the light. A dark current test can be real-timely performed. And there is no need to wait stabilization time. A real-time dark current value is deducted during processing the test data so that accuracy of absorbance can be raised.

Description

【Technical field】 [0001] The invention relates to a dark current detection method, which enables the microplate reader to deduct the influence of the dark current on the reading in real time when reading. 【Background technique】 [0002] In the medical field or optical research, it is usually necessary to measure some physical quantities of different wavelengths of light. The measurement method is usually to convert the optical signal into an electrical signal input, and use the current or voltage value to calculate the optical physical quantity. But when measuring, a current is detected even when no light passes through, and this current is called dark current. [0003] Microplate reader is an example. The microplate reader detects the absorbance value of the measured solution under a specific monochromatic light wavelength. Its optical system usually includes a light source, a filter wheel (built-in filter), an optical fiber, a microplate plate, and a photocell. and so on....

Claims

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Application Information

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IPC IPC(8): G01R19/00G01N21/31
Inventor 刘书刚李伟
Owner 上海永创生物工程有限公司
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