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Processing method for increasing lactobionic acid yield of pseudomonas fluorescence by applying physical field enhancement policy

A Pseudomonas fluorescens, processing method technology, applied in the field of food processing, to achieve the effect of promising market prospects, easy operation, and increased production

Inactive Publication Date: 2011-11-23
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research reports on ultra-high pressure processed food mainly focus on food sterilization and inactivation of enzymes, and so far there is no relevant report on the use of physical field strengthening strategies to promote high production of lactobionic acid by Pseudomonas fluorescens

Method used

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  • Processing method for increasing lactobionic acid yield of pseudomonas fluorescence by applying physical field enhancement policy
  • Processing method for increasing lactobionic acid yield of pseudomonas fluorescence by applying physical field enhancement policy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Activate and inoculate Pseudomonas fluorescens SK17.001 into liquid fermentation medium (in g / 100mL, lactose 3.0, yeast extract and / or peptone 0.5, potassium phosphate 0.2, magnesium sulfate 0.2, ferrous sulfate 0.005, adjust with distilled water to pH 6.2), cultured at 30oC, 200 rpm for 18 hours and then centrifuged to obtain resting cells; then the resting cells were placed in an autoclave under the conditions of OD600nm value 0.4, lactose concentration 4%, and pH 6.0. Middle; Finally, after treatment at 100MPa and 20°C for 30 minutes, it was determined that the synthetic product lactobionic acid increased by 3.7 times.

Embodiment 2

[0024] Activate and inoculate Pseudomonas fluorescens SK17.001 into liquid fermentation medium (in g / 100mL, lactose 5.0, yeast extract and / or peptone 3.5, potassium phosphate 0.2, magnesium sulfate 0.2, ferrous sulfate 0.005, pH 6.1) After culturing at 25oC and 350 rpm for 24 hours, the resting cells were obtained by centrifugation; then the resting cells were placed in a high-pressure reactor according to the concentration of the cells at an OD600nm value of 40, a lactose concentration of 4%, and a pH of 9.0; finally, at 50MPa, After treatment at 35°C for 10 minutes, the synthetic product lactobionic acid was determined to increase by 1.9 times.

Embodiment 3

[0026] Activate and inoculate Pseudomonas fluorescens SK17.001 into liquid fermentation medium (in g / 100mL, lactose 3.8, yeast extract and / or peptone 2.0, potassium phosphate 0.2, magnesium sulfate 0.2, ferrous sulfate 0.005, pH 6.0) After culturing at 40oC and 100 rpm for 12 hours, the resting cells were obtained by centrifugation; then the resting cells were placed in an autoclave according to the cell concentration of OD600nm value 12, lactose concentration 4%, and pH 4.0; finally, at 400MPa, Treated at 50°C for 60 minutes, the measured synthesis product lactobionic acid increased by 5.2 times.

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Abstract

The invention discloses a processing method for increasing lactobionic acid yield of pseudomonas fluorescence by applying a physical field enhancement policy, which belongs to the technical field of food processing. In the method, after being undergoing resting cell culture, the pseudomonas fluorescence synthesizes lactobionic acid in presence of a catalyst under stress caused by a high-pressure physical field. The processing method comprises the following steps: placing resting cells into a high-pressure reaction kettle according to requirements of biological processing; treating the restingcells under set temperature and pressure conditions for a certain period of time; and after the treatment is finished, regulating the pressure in the reaction kettle to normal temperature. In the invention, the lactobionic acid yield is increased by changing the permeability and structure of cell membrane with a high-pressure physical field technique; and compared with the conventional method, the method has a more obvious substrate conversion rate, adopts a simple processing process, realizes low production cost and meets requirements for industrial production.

Description

technical field [0001] The invention relates to a processing method for promoting the high production of lactobionic acid by Pseudomonas fluorescens by using a physical field strengthening strategy, and belongs to the technical field of food processing. Background technique [0002] Lactobionic acid, also known as the third-generation fruit acid, is the most advanced fruit acid with various excellent functions such as repairing, anti-aging, moisturizing, anti-oxidation and promoting body renewal. Lactobionic acid itself exists in the human body and has no irritation problem. Its excellent antioxidant capacity has long been used in organ transplantation to prevent organs from being damaged by free radicals and increase the preservation of organs; in medical cosmetology, it can avoid The cell membrane is damaged by oxidation; in terms of food processing, the US Food and Drug Administration (FDA) has approved lactobionic acid to be used as a bifidus factor, a mineral element ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12R1/39
Inventor 江波缪铭白会钗
Owner JIANGNAN UNIV
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