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Blattella germanica kdr mutant allele detection kit and special primers thereof

A technology of German cockroaches and kits, applied in the field of in situ gene detection kits and special primers, can solve the problems of no molding

Active Publication Date: 2013-01-16
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no currently formed kdr mutant allele detection method and kit for Blattella germanica

Method used

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  • Blattella germanica kdr mutant allele detection kit and special primers thereof
  • Blattella germanica kdr mutant allele detection kit and special primers thereof
  • Blattella germanica kdr mutant allele detection kit and special primers thereof

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Effect test

Embodiment 1

[0058] Embodiment 1, German sickle kdr mutation allele detection

[0059] 1. PCR amplification

[0060] 1. Design of primers

[0061] Design specific primers for the gene sequence kdr (unmutated, Accession number: U73583.1) of the sodium ion channel of the German cockroach:

[0062] CD1: 5'-GATATGCCGAGATGGAACTTTACG-3' (SEQ ID NO: 1, downstream primer)

[0063] CD2: 5'-AATATCCCTGACCAACCTGTGAA-3' (SEQ ID NO: 2, upstream primer)

[0064] CD3: 5'-CCACTGTCGTCATTGGAAACTTG-3' (SEQ ID NO: 3, downstream primer)

[0065] Specific primers designed according to the kdr mutant kdr L993F mutant gene (Accession number: U73584.1):

[0066] CD1: 5'-GATATGCCGAGATGGAACTTTACG-3' (SEQ ID NO: 1, downstream primer)

[0067] CD2: 5'-AATATCCCTGACCAACCTGTGAA-3' (SEQ ID NO: 2, upstream primer)

[0068] CD4: 5'-CCACTGT CGT CAT TGG AAA CTT C-3' (SEQ ID NO: 4, downstream primer)

[0069] 2. Genomic DNA acquisition

[0070] The genomic DNAs of German cockroaches numbered 1-3 captured in the wild wer...

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Abstract

The invention discloses a Blattella germanica kdr mutant allele detection kit and special primers thereof. The invention provides primers for detecting kdr gene mutant site, which are composed of a primer set A and a primer set B, wherein the primer set A comprises a primer 2 and a primer 3, and the primer set B comprises a primer 2 and a primer 4. The experiment proves that the invention provides a quick, simple and sensitive kit for detecting Blattella germanica drug resistance in order to master the drug resistance of Blattella germanica to pyrethroid insecticides.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for the kdr mutant allele of the German cockroach and special primers thereof. Background technique [0002] German cockroach (Blattella germanica) is one of the common indoor pests, which can carry bacteria, viruses, parasite eggs, etc., leading to the spread of various diseases. At the same time, German cockroach secretions or dead worms can cause body allergies. Chemical control is widely used because of its convenient implementation and quick effect. Among all kinds of chemical insecticides, pyrethroids are widely used in the control of agricultural pests and health pests because of their advantages of high efficiency, rapidity, low retention and low toxicity. Insecticide resistance. Due to the widespread use of relatively single chemical drugs for the control of German cockroaches, frequent use of drugs, and short life generations, the problem of drug resistanc...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 汪中明赵彤言李春晓董言德郭晓霞张映梅刘美德邢丹
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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