Method for increasing artemisinin content in Artemisia annua by salicylic acid treatment
A technology of artemisinin and salicylic acid, applied in the biological field, can solve the problems of low feasibility, difficulty, and high cost of artemisinin, and achieve the effects of facilitating large-scale production, reducing production costs, and increasing content
Inactive Publication Date: 2011-11-30
上海柏泰来生物技术有限公司
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Problems solved by technology
Large-scale commercial production of this drug is limited by the very low content of artemisinin in Artemisia annua (0.01%-1% dry weight)
Although it is possible to artificially synthesize artemisinin, it is not feasible for commercial production due to the difficulty, low yield and high cost.
[0003] The content of artemisinin in the callus is less than 0.1% of the dry weight, and the highest in the shoot is only 0.16% of the dry weight, and most studies have not detected artemisinin in the root, so the use of tissue culture and cell engineering The feasibility of producing artemisinin is not high
In addition, using genetic engineering technology to obtain new varieties of Artemisia annua with higher content of artemisinin is a more feasible method, but the use of genetic engineering to increase the content of artemisinin is still in the research stage, and there is no report on commercial production.
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Embodiment
[0012] The seeds of Artemisia annua used in this experiment were from the varieties of Artemisia annua with high artemisinin content in Xiangxi, Hunan. Sow Artemisia annua seeds at the end of February.
[0013] Sprinkle water to moisten the seedbed one day before sowing. When sowing, first mix the seeds into seed ash, spread evenly on the border, and then cover with a thin layer of plant ash to cover the seeds. Seedlings will emerge 10-15 days after sowing. Thin out the seedlings after emergence, properly pull out dense seedlings, thin seedlings, diseased seedlings, leggy seedlings or deformed seedlings, remove seedlings and weeds of other species mixed in them, and keep seedlings at a distance of about 10cm. When the Artemisia annua seedlings grow to 10-15cm, they are transplanted and planted in the field. Before transplanting, combined with deep plowing and plowing, apply 50 kg of nitrogen, phosphorus, and potassium compound fertilizers, and pour enough root-fixing water af...
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The invention belongs to the field of biotechnology, and specifically relates to a method for increasing the content of artemisinin in Artemisia annua through salicylic acid treatment. According to the invention, the salicylic acid solution is sprayed on the Artemisia annua plants 6-8 days before flowering, so that the content of artemisinin in the Artemisia annua can be increased. The invention has simple operation, can effectively increase the content of artemisinin in the artemisia annua, and has great significance for reducing the production cost of artemisinin.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for increasing the content of artemisinin in Artemisia annua. Background technique [0002] Artemisinin is currently the most effective antimalarial drug. The main source of artemisinin is extracted from the aerial part of the Artemisia annua plant. Artemisia annua ( Artemisia annua L.), also known as Artemisia annua, is an annual herbal medicinal plant. The large-scale commercial production of this drug is limited by the very low content of artemisinin in Artemisia annua (0.01%-1% dry weight). Although it is possible to artificially synthesize artemisinin, it is not feasible for commercial production due to the difficulty, low yield and high cost. [0003] The content of artemisinin in the callus is less than 0.1% of the dry weight, and the highest in the shoot is only 0.16% of the dry weight, and most studies have not detected artemisinin in the root, so t...
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IPC IPC(8): A01G7/06A01N37/40A01P21/00
Inventor 孙光新叶忠
Owner 上海柏泰来生物技术有限公司