A preparation method of adipose tissue breast augmentation material enriched with autologous stem cells

Abstract

The invention belongs to the field of biomedicine, and particularly discloses a preparation method of an adipose tissue breast augmentation material enriched with autologous stem cells. In the present invention, only 5ml of tissue is extracted from the patient, and a large number of adipose stem cells are obtained through separation, purification, and in vitro culture and expansion, and finally added to the newly prepared SVF components, mixed with adipose tissue for breast augmentation transplantation. The invention can reduce the amount of adipose tissue used for preparing SVF during fat transplantation, so as to make up for the disadvantage that a large number of women cannot obtain a large amount of adipose tissue to prepare SVF due to low body mass index and cannot perform autologous fat breast augmentation.

Description

(1) Technical field [0001] The invention belongs to the field of biomedicine, in particular to a preparation method of adipose tissue breast augmentation material enriched with autologous stem cells. (2) Background technology [0002] Breast augmentation or mammary augmentation (mammary augmentation) is the most common cosmetic surgery in plastic surgery. Since its birth in the early 20th century, filling materials have become the focus of attention. Many breast augmentation materials have been tried, such as paraffin wax, silicon wax, etc. Gel, saline, autologous dermal fat flap, etc. However, long-term medical practice has proved that all types of tissue substitutes have more or less discomfort and complications (including contracture, deformation, hardening, and leakage). It brings great confusion to medical seekers and doctors. Therefore, in the late 1970s, some people began to try to use autologous fat tissue for breast augmentation on the basis of dermal fat f...

AI Technical Summary

Problems solved by technology

If adipose stromal cells (ASCs) are relatively deficient in the aspirated adipose tissue, it will affect the replacement process and lead to postoperative lipoatrophy

This is also the reason why the breast augmentation effect of simple fat particle transplantation cannot last long. Therefore, in order to obtain a long-lasting breast augmentation effect, it is necessary to supplement the number of adipose stromal cells (ASC) or adipose stem cells to the inhaled adipose tissue with insufficient progenitor cells to increase the amount of transplanted fat. survival rate