A kind of cultivation method of caproic acid bacteria and liquid pit mud mixed bacterial solution

A culturing method and a technology for mixing bacterial liquid, which are applied in the field of winemaking and can solve the problems of poor use effect, cumbersomeness and instability of caproic acid bacteria and liquid pit mud.

Active Publication Date: 2011-11-30
SICHUAN MIANYANG FORGOOD DISTILLERY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is: how to provide a culture method of caproic acid bacteria and liquid pit mud mixed bacterial solution, which solves the problem that artificially added calcium substances enter the pit mud to promote the aging of the pit mud and in the cylinder type cultivation The problems of cumbersome operation, limited quality and instability, at the same time, also solve the problem of poor effect of caproic acid bacteria and liquid pit mud alone

Method used

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  • A kind of cultivation method of caproic acid bacteria and liquid pit mud mixed bacterial solution
  • A kind of cultivation method of caproic acid bacteria and liquid pit mud mixed bacterial solution
  • A kind of cultivation method of caproic acid bacteria and liquid pit mud mixed bacterial solution

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Caproic acid bacteria medium formula: NaAC: 0.5%, (NH 4 ) 2 SO 4 : 0.05%, K 2 HPO 4 : 0.04%, MgSO 4 : 0.02%, waste extract: 5%, yeast extract: 0.1%, 95% alcohol: 2%, water: 92.29%;

[0031] Liquid Pit Mud Medium Formula: Lost Grass Extract: 50%, Daqu Powder: 2%, Bottom Pot Water: 2%, K 2 HPO 4 : 0.3%, NaAC: 0.3%, 95% alcohol: 2%, yellow water: 3%, water: 40.4%;

[0032] Inoculate the Karst tank with 1% caproic acid bacteria and 9% liquid cellar mud strains, the inoculation amount of stainless steel seed tank and stainless steel fermentation tank is 10%, and the Karst tank, stainless steel seed and stainless steel fermentation tank are all used in liquid cellar Mud medium.

[0033] Cultivation of caproic acid bacteria in a triangular flask: A. Fill a 500mL triangular flask with 440mL caproic acid culture medium except 95% alcohol; B. Autoclave the triangular flask at 0.1Mpa for 30min, cool to 32°C, Inoculate 50 mL caproic acid bacterial seed solution, add 10 mL ...

Embodiment 2

[0040] Caproic acid bacteria medium formula: NaAC: 0.5%, (NH 4 ) 2 SO 4 : 0.05%, K 2 HPO 4 : 0.04%, MgSO 4 : 0.02%, waste extract: 4.29%, yeast extract: 0.1%, 95% alcohol: 4%, water: 91%;

[0041] Formula of liquid pit mud medium: dregs soaking liquid: 45%, Daqu powder: 2%, bottom pot water: 2%, K 2 HPO 4 : 0.3%, NaAC: 0.3%, 95% alcohol: 4%, yellow water: 4.6%, water: 45%;

[0042] Inoculate the Karst tank with 1% caproic acid bacteria and 9% liquid cellar mud strains, the inoculation amount of stainless steel seed tank and stainless steel fermentation tank is 10%, and the Karst tank, stainless steel seed and stainless steel fermentation tank are all used in liquid cellar Mud medium.

[0043] Cultivation of caproic acid bacteria in a triangular flask: A. Fill a 500mL triangular flask with 430mL caproic acid culture medium except 95% alcohol; B. Autoclave the triangular flask at 0.1Mpa for 30min, cool to 32°C, Inoculate 50 mL caproic acid bacterial seed solution, add 2...

Embodiment 3

[0050] Caproic acid bacteria medium formula: NaAC: 0.5%, (NH 4 ) 2 SO 4 : 0.05%, K 2 HPO 4 : 0.04%, MgSO 4 : 0.02%, waste extract: 3%, yeast extract: 0.1%, 95% alcohol: 2.29%, water: 94%;

[0051] Liquid Pit Mud Medium Formula: Lost Grass Extract: 49.6%, Daqu Powder: 2%, Bottom Pot Water: 2%, K 2 HPO 4 : 0.3%, NaAC: 0.3%, 95% alcohol: 3.8%, yellow water: 5%, water: 37%;

[0052] Inoculate Karst tanks with 1% caproic acid bacteria and 9% liquid cellar mud strains. The inoculum amount of stainless steel seed tanks and stainless steel fermentation tanks is 10%. Mud medium.

[0053]Cultivation of caproic acid bacteria in a triangular flask: A. Fill a 500mL triangular flask with 438.55mL caproic acid culture medium except 95% alcohol; B. Autoclave the triangular flask at 0.1Mpa for 30min and cool to 32°C , inoculate 50 mL of caproic acid bacterial seed solution, add 11.45 mL of 95% alcohol, seal with a silica gel plug, and incubate at 30-32°C for 7 days. the

[0054] Tri...

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Abstract

The invention discloses a hexanoic acid bacterium and liquid pig mud bacterium mixed bacterial solution culture method, which comprises: culturing hexanoic acid bacteria in a hexanoic acid bacterium culture medium and liquid pig mud bacteria in a liquid pig mud culture medium separately; inoculating 1 percent of hexanoic acid bacterium strain and 9 percent of liquid pig mud bacterium strain into a Carlsberg flask, a stainless stain seed tank and a stainless stain fermentation tank in turn; and thus, obtaining the hexanoic acid bacterium and liquid pig mud bacterium mixed bacterial solution. The hexanoic acid bacterium and liquid pig mud bacterium mixed bacterial solution prepared by the method, when used for maintaining pig mud and culturing artificial pig mud, can solve the problem that the artificial addition of calcium matters into the pig mud promotes the aging of the pig mud and the problem of complex operation, low and instable quality in tank-type culture and also solve the problem of undesirable use effect of hexanoic acid bacteria and liquid pig mud bacteria, which are used alone.

Description

technical field [0001] The invention relates to the technical field of brewing, in particular to a method for cultivating caproic acid bacteria and a mixed bacterial solution of liquid pit mud. Background technique [0002] In Luzhou-flavor Daqu liquor, the biochemical synthesis of ethyl caproate, the main aroma substance, is formed by the condensation of caproic acid metabolized by caproic acid bacteria in the pit mud and ethanol in the fermented grain system. Therefore, the quantity and quality of Paenibacillus caproic acid bacteria in the pit mud have a great relationship with the wine quality. In the cultivation of artificial pit mud and the maintenance of pit mud, the artificial cultivation of functional bacteria in pit mud plays an important role, and the cultivation methods are generally the cultivation of caproic acid bacteria and the cultivation of liquid pit mud. However, when artificially cultivating functional bacteria in pit mud, a large amount of calcium subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/145
Inventor 杜礼泉唐聪
Owner SICHUAN MIANYANG FORGOOD DISTILLERY
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