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A kind of preparation method of bacterial cellulose support material and its products

A technology of bacterial cellulose and bacterial cellulose membrane, which is applied in the field of preparation of bacterial cellulose scaffold materials and its products, can solve the problems of long fermentation time, inability to prepare porous scaffold materials, complicated process, etc., and achieve low cost and excellent preparation process. Simple and easy to operate effect

Inactive Publication Date: 2011-12-14
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are problems that the fermentation time is longer, the process is more complicated, and it is difficult to completely remove the pore-forming agent in the scaffold material.
However, the sodium chloride crystal particles are usually uniformly dispersed in the unshaped raw material before the porous scaffold material can be prepared, while the bacterial cellulose membrane is a shaped product, if it is dissolved or beaten, the mechanical properties of the bacterial cellulose membrane will meet the requirements. Without the requirements of the scaffold material, sodium chloride crystal particles cannot be prepared by uniformly dispersing the dissolved or beaten bacterial cellulose raw material to prepare a porous scaffold material. An effective method for porous scaffold materials, and obtain a bacterial cellulose scaffold material with micron-scale macropores uniformly distributed in a three-dimensional network structure of bacterial cellulose with controllable pore size

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029]Soak the bacterial cellulose membrane in 40% saturated sodium chloride aqueous solution at 20°C, let it stand or stir for 30 minutes, then take out the treated bacterial cellulose membrane, and cool it down to -20°C within 1 minute. The bacterial cellulose membrane is frozen into a solid state and then freeze-dried; then, sodium chloride is removed by washing at a temperature of 20°C and a washing time of 30 minutes until chloride ions no longer appear in the solution after washing, and the three-dimensional network of bacterial cellulose nanometers is obtained. Bacterial cellulose scaffold materials with 50-70 μm macropores are evenly distributed in the structure;

Embodiment 2

[0031] Soak the bacterial cellulose membrane in 60% saturated sodium chloride aqueous solution at 50°C, let it stand or stir for 30 minutes, then take out the treated bacterial cellulose membrane, and cool it down to -80°C within 1 minute. The bacterial cellulose membrane was frozen into a solid state and then freeze-dried; then washed to remove sodium chloride, and then washed to remove sodium chloride, the washing temperature was 30 ° C, and the washing time was 50 minutes, until chloride ions no longer appeared in the solution after washing. That is, the bacterial cellulose scaffold material with 50-100 μm macropores evenly distributed in the bacterial cellulose nanometer three-dimensional network structure is obtained;

Embodiment 3

[0033] Soak the bacterial cellulose membrane in 80% saturated sodium chloride aqueous solution at 80°C, let it stand or stir for 30 minutes, then take out the treated bacterial cellulose membrane, cool it down to -120°C within 1 minute, and wait for The bacterial cellulose membrane is frozen into a solid state and then freeze-dried; then the sodium chloride is removed by washing, and the sodium chloride is removed by washing, the washing temperature is 60°C, and the washing time is 60min, until no chloride ions appear in the washed solution. That is, the bacterial cellulose scaffold material with 50-140 μm macropores evenly distributed in the bacterial cellulose nanometer three-dimensional network structure is obtained;

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Abstract

The invention belongs to the field of biomedical materials, and relates to a method for preparing a bacterial cellulose scaffold material and its products, in particular to a bacterial cellulose scaffold material in which micron-scale macropores are uniformly distributed in a bacterial cellulose nano-scale three-dimensional network structure The preparation method and its products, specifically, the method and its products are obtained by using sodium chloride crystals as a porogen to obtain a bacterial cellulose scaffold material with 50-250 μm macropores uniformly distributed in the bacterial cellulose nanometer three-dimensional network structure. The method of the present invention prepares a biomedical scaffold material by using sodium chloride crystals as a porogen to form a porous structure on the formed bacterial cellulose membrane on the basis of insoluble, beating and dispersed bacterial cellulose membrane, and the preparation process is simple Easy operation, convenient operation, no pollution and low cost.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and relates to a method for preparing a bacterial cellulose scaffold material and its products, in particular to a bacterial cellulose scaffold material in which micron-scale macropores are uniformly distributed in a bacterial cellulose nano-scale three-dimensional network structure The preparation method and its products, specifically, use sodium chloride crystals as a porogen to obtain a bacterial cellulose scaffold material with micron-scale macropores evenly distributed in the bacterial cellulose nano-scale network structure and its products. Background technique [0002] Tissue engineering is the principle and method of applying engineering and life sciences. Functionally related living cells cultured and expanded in vitro are planted on porous scaffolds. Cells proliferate and differentiate on the scaffolds to construct biological substitutes, and then transplant them into Tissue damage s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/20
Inventor 尹娜李喆王华平陈仕艳洪枫欧阳炀杨敬轩徐秋舒
Owner DONGHUA UNIV