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A method for increasing α-linolenic acid content in transgenic plant seeds

A linolenic acid and transgenic technology, applied in botany equipment and methods, biochemical equipment and methods, plant products, etc., can solve problems such as low α-linolenic acid content, waste, and increase the metabolic burden of transgenic plants, and achieve large applications foreground effect

Inactive Publication Date: 2011-12-14
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the use of constitutive promoters to drive the high-intensity expression of the target gene in the entire period of plant growth and development and in all plant tissues will bring potential adverse effects, such as: increasing the metabolic burden of transgenic plants and unnecessary waste, etc., while the target organ seeds The alpha-linolenic acid content in

Method used

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  • A method for increasing α-linolenic acid content in transgenic plant seeds
  • A method for increasing α-linolenic acid content in transgenic plant seeds
  • A method for increasing α-linolenic acid content in transgenic plant seeds

Examples

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Effect test

Embodiment 1

[0046] Example 1. Using an endosperm-specific promoter to express the rice ω-3 fatty acid dehydrogenase gene to increase the content of α-linolenic acid in the seeds of transgenic plants

[0047] 1. Acquisition of rice omega-3 fatty acid dehydrogenase gene

[0048] The nucleotide sequence of rice ω-3 fatty acid dehydrogenase was searched from GenBank (GenBank number is AK071185), and primers were designed to amplify the rice ω-3 fatty acid dehydrogenase gene (OsFAD3). To facilitate vector construction, two restriction sites (underlined) were sequentially added to each pair of primers.

[0049] Rice ω-3 fatty acid dehydrogenase gene amplification primer: forward primer is F2: 5′-AA CCCGGG ATGGTTAAAGACACAAAG-3′( SmaI ) (sequence 1), the reverse primer is R2: 5'-AA GAGCTC TCAGTCTCGTTGCGAGTGGA-3′( Sac I ) (sequence 2).

[0050] Using the Trizol kit (purchased from Invitrogen), from rice Kitaake 12 days after flowering (Oryza sativaL.cv.Kitaake, Qu and Takaiwa, Plant Biote...

Embodiment 2

[0084] Example 2. Using an endosperm-specific promoter to express soybean ω-3 fatty acid dehydrogenase gene to increase the content of α-linolenic acid in transgenic plant seeds

[0085] 1. Acquisition of soybean omega-3 fatty acid dehydrogenase gene

[0086] Search soybean ω-3 fatty acid dehydrogenase nucleotide sequence from GenBank (GenBank number is AY204710), design primers to amplify soybean ω-3 fatty acid dehydrogenase gene (GmFAD3). To facilitate vector construction, two restriction sites (underlined) were sequentially added to each pair of primers.

[0087] Primers for soybean ω-3 fatty acid dehydrogenase gene amplification: the forward primer is F3: 5′-AACCCGGGATGGCGGCGTCGGCGACCCAG-3′ (Sma I), and the reverse primer is R3: 5′-AAGAGCTCTCACTTGTGCTTAGCATCTTC-3′ (Sac I).

[0088] Using the Trizol kit (purchased from Invitrogen), total RNA was extracted from soybean leaves 12 days after flowering (Shennong 25104, a gift from Professor Yu Jianzhang of Shenyang Agricultura...

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Abstract

The invention discloses a method for improving alpha-linolenic acid content in transgenic plant seeds. The method comprises the steps that: an expression box for specific expression of an omega-3 fatty acid dehydrogenase gene by endosperm is introduced into a plant, and a transgenic plant that has specific expression of the omega-3 fatty acid dehydrogenase gene in endosperm can be obtained through screening; the expression box for specific expression of an omega-3 fatty acid dehydrogenase gene by endosperm comprises a plant endosperm specific promoter and an omega-3 fatty acid dehydrogenase gene connected to the downstream of the promoter. The method of the invention can improve seed quality, and is applicable to large scale production of alpha-linolenic acid and cultivation of transgeniccrops that can be inherited stably and have seeds with high alpha-linolenic acid content.

Description

technical field [0001] The invention relates to a method for increasing the content of α-linolenic acid in transgenic plant seeds, in particular to a method for increasing the content of α-linolenic acid in transgenic plant seeds by using an endosperm-specific promoter to express ω-3 fatty acid dehydrogenase gene. Background technique [0002] Unsaturated fatty acids are essential fatty acids for the human body. Unsaturated fatty acids are divided into monounsaturated fatty acids and polyunsaturated fatty acids according to the number of double bonds. According to the position and function of the double bond, polyunsaturated fatty acids are divided into ω-3 series and ω-6 series. α-linolenic acid, EPA, DHA belong to omega-3 series, linoleic acid, γ-linolenic acid and arachidonic acid belong to omega-6 series. These long-chain polyunsaturated fatty acids are components of biological membranes of body tissues and some important organs (such as: cerebral cortex, nerve tissue ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/113C12N15/53C12Q1/68G01N33/53G01N30/02A01H5/00
Inventor 曲乐庆刘华梁
Owner INST OF BOTANY CHINESE ACAD OF SCI
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