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Simple industrial production method for dendrobium candidum

A technology of dendrobium candidum and its production method, which is applied in the field of simple chemical plant production of dendrobium candidum seedlings, can solve the problems of low survival rate, low yield and slow growth of wild dendrobium candidum seeds, achieve considerable economic and social benefits, and reduce carbon The effect of emissions

Active Publication Date: 2013-06-26
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The survival rate of wild Dendrobium officinale seeds is extremely low, and the growth is slow. It takes 5-7 years to harvest from seed germination to the commercial stage, resulting in very little yield

Method used

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  • Simple industrial production method for dendrobium candidum
  • Simple industrial production method for dendrobium candidum
  • Simple industrial production method for dendrobium candidum

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Embodiment

[0024] In April 2005, diploid Dendrobium officinale (2n=38) was introduced from Wenshan, Yunnan Province, as figure 1 , artificial pollination of the same plant with different flowers was carried out on May 20 of that year. figure 2 , cut off the capsule, in the ultra-clean workbench, first disinfect with 75% alcohol for 1 minute, then disinfect with 0.1% mercuric chloride for 10 minutes, rinse with sterile water 5 times, and cut along the longitudinal axis of the capsule with a sterile scalpel , the seeds of Dendrobium officinale were inoculated in 1 / 2MS liquid medium and cultured with shaking at 25°C. After about 20 days, all the seeds germinated, as image 3 . Then transfer to 1 / 2MS solid medium to carry out protocorm induction culture, such as Figure 4 . Figure 5 Show that after the seed class protocorms are differentiated, they are transferred and inoculated in PP plastic bags, and each bag is inoculated with 10-15 seedlings, such as Figure 8 , Seal the mouth of t...

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Abstract

The invention relates to a simple industrial production method for dendrobium candidum, which comprises the following steps: carrying out the hybrid pollination of dendrobium candidum according to the conventional method; performing the non-symbiosis germination culture of the seeds of dendrobium candidum; subjecting the protocorm-like bodies (PLBs) of the seeds to induction differentiation on a 1 / 2 Murashige and Skoog (MS) culture medium; inoculating the differentiated protocorms into polypropylene (PP) plastic bags and performing seedling raising differentiated culture; and transplanting seedlings 3 to 4 months later. In the invention, a new variety is provided for agriculture production in green houses in rural areas, the industrial batch production of dendrobium candidum seedlings is realized, natural heating and lighting reduce carbon emission and prevent secondary pollution, the plastic bags are suspended in the green houses for fully utilizing effective space, millions of dendrobium candidum seedlings can be grown in each mu in the green houses, and thus, the method has very considerable economic and social benefit.

Description

technical field [0001] The invention relates to a simple chemical factory production method for dendrobium officinale seedlings. Background technique [0002] Dendrobium candidum Wall.ex Lindl, also known as black knot grass, is an epiphytic, perennial herbaceous orchid plant. Mainly distributed in my country's Yunnan, Guizhou, Guangxi, Taiwan and other places. The annual average temperature is 12-18 degrees, the relative humidity is 60-75%, the light transmittance in the forest is about 60%, and the annual rainfall is 1100-1500mm. Grows in evergreen broad-leaved forests, epiphytic on tree trunks or limestone. The survival rate of wild Dendrobium candidum seeds is extremely low, and the growth is slow. It takes 5-7 years to harvest from seed germination to entering the commercial stage, resulting in very little yield. In recent years, the natural resources of Dendrobium candidum have been depleted day by day due to the shortage of supply in the market, over-excavation by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 李秀兰陈力陈瑞阳
Owner NANKAI UNIV
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