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Composite microbial enzyme preparation and preparation method thereof

A technology of compound microorganisms and enzyme preparations, applied in the field of microorganisms, can solve problems such as unsuitable conditions, insufficient substrate concentration, and insignificant application effects, and achieve the effect of improving the quality of liquor and having broad application prospects.

Active Publication Date: 2013-03-06
LUZHOU PINCHUANG TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application effect in fermented grains or filling cellars is not obvious (no difference from the control), the specific reason may be that the initial concentration of the substrate is not enough, or it is affected by other microorganisms, or caused by unsuitable conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 Adopt the method of the present invention to prepare the compound microbial enzyme preparation for brewing

[0033] 1. Preparation of caproic acid bacteria solution

[0034] Put caproic acid bacteria into sterile water at a ratio of 1:4. After 8-10 minutes of treatment in a hot water bath at 80°C, inoculate the caproic acid bacteria seeds in the caproic acid bacteria seed culture medium. The culture condition is that the temperature is 34 °C, the pH value is 6, and the culture time is 7-8 days. When the culture solution turns pale yellow and small bubbles rise, and the bacteria are in a vigorous metabolic period, expand the culture.

[0035] Cultivation of caproic acid bacteria liquid for production: Rinse the 10L earthenware pot with water → fermented grain filtrate, sodium acetate and ammonium sulfate (the amount added is half of the container) → add steam and heat to boil, keep for 30 minutes → wait for the altar to cool to 40 ℃, add high-temperature s...

Embodiment 2

[0047] Example 2 Using the method of the present invention to prepare pit mud functional bacteria

[0048] 1. Preparation of caproic acid bacteria solution

[0049] Put caproic acid bacteria into sterile water at a ratio of 1:4. After 8-10 minutes of treatment in a hot water bath at 80°C, inoculate the caproic acid bacteria seeds in the caproic acid bacteria seed culture medium. The culture condition is that the temperature is 34 °C, the pH value is 6.5, and the culture time is 7-8 days. When the culture solution turns pale yellow and small bubbles rise, and the bacteria are in a vigorous metabolic period, expand the culture.

[0050] Cultivation of caproic acid bacteria liquid for production: Rinse the 10L earthenware pot with water → fermented grain filtrate, sodium acetate and ammonium sulfate (the amount added is half of the container) → add steam and heat to boil, keep for 30 minutes → wait for the altar to cool to 40 ℃, add high-temperature sterilized calcium carbonate...

Embodiment 3

[0062] Example 3 Using the method of the present invention to prepare pit mud functional bacteria

[0063] 1. Preparation of caproic acid bacteria solution

[0064] Put caproic acid bacteria into sterile water at a ratio of 1:4. After 8-10 minutes of treatment in a hot water bath at 80°C, inoculate the caproic acid bacteria seeds in the caproic acid bacteria seed culture medium. The culture condition is that the temperature is 34 °C, the pH value is 6, and the culture time is 7-8 days. When the culture solution turns pale yellow and small bubbles rise, and the bacteria are in a vigorous metabolic period, expand the culture.

[0065] Cultivation of caproic acid bacteria liquid for production: Rinse the 10L earthenware pot with water → fermented grain filtrate, sodium acetate and ammonium sulfate (the amount added is half of the container) → add steam and heat to boil, keep for 30 minutes → wait for the altar to cool to 40 ℃, add high-temperature sterilized calcium carbonate a...

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PUM

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Abstract

The invention relates to a composite microbial enzyme preparation and a preparation method thereof and belongs to the field of microbes. The technical problem to be solved by the invention is to provide the method for producing the composite microbial enzyme preparation which can improve fermentation fragrance of distiller's grains for improving quality of produced white liquor. The method for producing the composite microbial enzyme preparation comprises the following steps: a, preparing hexanoic acid bacterial solution; b, preparing pit mud enriched bacterial solution; c, preparing pit mud functional bacteria, wherein the hexanoic acid bacterial solution and pit mud enriched bacterial solution are mixed and inoculated into a pit mud functional bacterium culture medium, the bacteria are cultured at 32 to 36 DEG C for 22 to 27 days to form pit mud functional bacterium solution, the pit mud functional bacterium solution is dried to prepare the pit mud functional bacteria; and d, mixingdry pit mud functional bacteria, fragrance producing yeasts, B type enzymatic matters and red starter esterified enzyme in a dry weight ratio of 1:(0.25-0.35):(1-2):(0.30-0.45) and thus, obtaining the composite microbial enzyme preparation.

Description

technical field [0001] The invention relates to a compound microbial enzyme preparation and a preparation method thereof, belonging to the field of microorganisms. Background technique [0002] With its unique solid-state fermentation process and product style, traditional Chinese liquor occupies an important position among the six major distilled spirits in the world. Lu-type liquor, as a representative of Luzhou-flavor liquor, occupies a huge market share in the Chinese liquor market and has become the most influential model of Chinese liquor. [0003] The unique production process of Luzhou-flavor koji liquor is characterized by "solid-state fermentation in mud cellar, continuous grain ingredients, mixed steaming and burning". Pit mud is the basis for the brewing of Luzhou-flavor koji wine, and its quality directly affects the quality of Luzhou-flavor liquor. The microbial flora in the pit mud is extremely complex, inhabiting a large number of caproic acid bacteria, but...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01C12R1/865C12G3/02
Inventor 张宿义方军易彬卢中明敖宗华
Owner LUZHOU PINCHUANG TECH CO LTD
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