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Papanicolaou staining solution and its rapid staining method

A pasteurized staining and rapid staining technology, applied in the field of pasteurized staining solution and its dyeing, can solve the problems of difficult operation, difficult to master, and long time for inspection technicians, and achieves simple and reliable staining method, improved work efficiency, and improved preparation. simple method effect

Active Publication Date: 2011-12-21
ANHUI CHAOHU HONGCI MEDICAL DEVICES
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  • Abstract
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Problems solved by technology

[0004] The preparation of traditional Papanicolaou staining solution is complicated, the staining procedures are complicated, the time is long and difficult to master
Therefore, inspection technicians generally feel that the operation is difficult

Method used

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  • Papanicolaou staining solution and its rapid staining method
  • Papanicolaou staining solution and its rapid staining method

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Embodiment Construction

[0028] In this embodiment, the Papanicolaou staining solution is composed of a nuclear staining solution and a cytoplasmic staining solution;

[0029] Preparation of cell nucleus staining solution:

[0030] Separately dissolve 0.3g of hematoxylin color essence in 30ml of absolute ethanol to obtain hematoxylin alcohol liquid; put 6g of ammonium aluminum sulfate in a 1000ml large beaker, add 700ml of distilled water, heat to 70-80°C, stir to dissolve completely, and then heat To 90°C, turn off the heat source and add hematoxylin alcohol solution, stir while adding, continue to heat until boiling and leave the heat source; then add 0.5g yellow mercuric oxide powder to it, stir while adding, continue heating to make the solution pass through It is dark purple by visual inspection, immediately placed in water at 10-30°C for cooling in a water bath; after cooling to 10-30°C, place it in a plastic bucket, add 2ml of glacial acetic acid with a volume percentage of 1%, and filter the r...

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Abstract

The invention discloses a papanicolaou staining liquid and a fast staining method of the papanicolaou staining liquid. The papanicolaou staining liquid consists of cell nucleus staining liquid and cytoplasm staining liquid. The fast staining method of the papanicolaou staining liquid is operated in 22-DEG C room-temperature environment. A preparation method of the papanicolaou staining liquid is simple, and the cost is low. The staining method using the papanicolaou staining liquid is simple and reliable, the traditional 90 minutes can be reduced to 7 minutes, and the work efficiency is greatly improved.

Description

technical field [0001] The invention relates to Papanicolaou staining solution and its staining method, belonging to in vitro diagnostic reagents and reagent usage methods Background technique [0002] Papanicolaou staining is the best and most commonly used staining method in pathological sections and exfoliated cells. This staining method not only has the characteristics of clear nucleus structure, obvious color separation, good transparency, and bright cytoplasm, but also The dyed specimens are not easy to decolorize and can be stored for a long time. [0003] The main component of the nucleus is deoxyribonucleic acid, and its isoelectric point is pH 1.6-2.0. When the pH is greater than 2.0, DNA is negatively charged and can combine with positively charged dye cations. Hematoxylin is a dye for dyeing nuclei. After oxidation, it becomes oxidized hematoxylin, that is, hematoxylin red or hematoxylin. Its plasma point is PH 6.5. When the pH of the dye solution is adjusted to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N1/30
Inventor 吴志昇袁立成吴松
Owner ANHUI CHAOHU HONGCI MEDICAL DEVICES
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