Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain-specific qualitative and quantitative PCR detection method for transgenic carnation moonlite

A detection method, carnation technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., to achieve high sensitivity and strong specificity

Inactive Publication Date: 2011-12-21
SHANGHAI ACAD OF AGRI SCI
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic scientific research institutions have not conducted research on the detection technology of transgenic carnation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain-specific qualitative and quantitative PCR detection method for transgenic carnation moonlite
  • A strain-specific qualitative and quantitative PCR detection method for transgenic carnation moonlite
  • A strain-specific qualitative and quantitative PCR detection method for transgenic carnation moonlite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0043] The technical solution of the present invention will be further described in detail below in conjunction with specific embodiments.

[0044] The present invention takes transgenic carnation Moonlite strain specific detection research as an example,

[0045] The specific method is as follows:

[0046] 1. Extract the genomic DNA of transgenic carnation Moonlite.

[0047] Genomic DNA of transgenic Carnation Moonlite was extracted using the plant genome extraction kit "Plant DNA Mini-prep kit" (Ruifeng Biotechnology Co., Ltd., Shanghai). Utilize spectrophotometer (Thermo Company EV60) to measure DNA concentration and purity to be 10ng / μL-100ng / μL and OD260 / 280=1.7-2.0 respectively, according to carnation genome DNA size (Royal botanic Gardens, Kew.Plant c-DNA value The database (release 4.0, October 2005) determined its copy number to be 15873-158730 copy / μL.

[0048] 2. Amplification and confirmation of the adjacent sequence on the left side of the foreign gene insertio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a qualitative and quantitative PCR (polymerase chain reaction) detection method for strain specificity of transgenic carnation Moonlite, which comprises the following steps: 1) extraction of genome DNA (deoxyribonucleic acid) of the transgenic carnation Moonlite; 2) amplification and confirmation of the left boundary adjacent sequence of the transgenic carnation Moonlite exogenous gene insertion site; 3) establishment and verification of the qualitative PCR detection method for transgenic carnation Moonlite; and 4) establishment and verification of the quantitative PCRdetection method for transgenic carnation Moonlite. The invention successfully establishes the qualitative and quantitative PCR detection method for strain specificity of transgenic carnation Moonlite, and verifies the specificity and sensitivity of the detection method, thereby providing references for import detection, supervision and environmental safety evaluation of transgenic carnation.

Description

technical field [0001] The invention belongs to the technical field of transgenic plant detection, and in particular relates to a strain-specific qualitative and quantitative PCR detection method of transgenic carnation Moonlite. Background technique [0002] Since the first transgenic tomato FLAVR SAVR was approved by the USDA for commercial production in 1994, more and more transgenic plants have been used in agricultural production. The industrialization of genetically modified plants, especially the industrialization of genetically modified crops can reduce the impact of agriculture on the environment, increase food production, and help solve the global food problem. According to statistics, during the 15 years from 1996 to 2010, the total area of ​​GM crops planted exceeded one billion hectares. The planting area of ​​genetically modified crops increased 87 times from 17,000 hectares in 1996 to 1.48 million hectares in 2010. In 2010, the number of countries planting g...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 潘爱虎李鹏贾军伟蒋玲曦朱宏白蓝王金斌唐雪明
Owner SHANGHAI ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com