Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tube pressing-type chronic nerve root injury model in animal and constructing method and application thereof

A construction method and animal model technology, applied in veterinary instruments, veterinary surgery, medical science, etc., to achieve the effect of easy replication, easy comparison, and unified pressure

Inactive Publication Date: 2012-01-04
SHANGHAI HOSPITAL OF TRADITIONAL CHINESE MEDICINE
View PDF0 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a standard, stable animal model of tube pressure chronic nerve root injury that can comprehensively study and describe the characteristics of pathological changes in nerve roots, ganglia, spinal cord, etc., and its construction methods and applications are still unknown. No report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tube pressing-type chronic nerve root injury model in animal and constructing method and application thereof
  • Tube pressing-type chronic nerve root injury model in animal and constructing method and application thereof
  • Tube pressing-type chronic nerve root injury model in animal and constructing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Construction of Chronic Nerve Root Injury Animal Model

[0031] 1. Experimental method

[0032] 1. Experimental animals:

[0033] Eight-week-old male SD rats with a body weight of 200-300 grams, 5 in each group; they were uniformly raised in plastic cages, and the rats were allowed to drink water and eat freely. The laboratory light is 06:00-18:00, the temperature is 22-24°C, and the humidity is 40-60%.

[0034] 2. Modeling method:

[0035] All animal operations were performed by the same physician to ensure the technical quality of the operation and the comparability between groups, and eliminate or reduce the technical errors caused by the operation.

[0036] Anesthetized by intraperitoneal injection of ketamine (0.10-0.12g / kg), under the operating microscope in L 5 / L 6 With the intervertebral space as the center, a posterior median incision was taken and incision was made layer by layer, and the left inferior articular process of L5 lumbar vertebrae...

Embodiment 2

[0065] In terms of making lumbar nerve root compression injury models, domestic and foreign scholars have done different animal models, but a standard and stable model has not been formed.

[0066] Wang Yongjun et al. used the method of placing 30±1.5mg of silica gel sheet in the axilla at the junction of the L5 nerve root and the dural sac to make an animal model of nerve compression. (For details, see: Wang Yongjun, Wan Chao. Model establishment of experimental lumbar nerve root compression. Chinese Journal of Orthopedics and Traumatology [J], 1999, 7(1): 9-12.) Liu Jinxian and others will have a length of 3.0mm and an inner diameter of A 1.0mm, 1mm-thick silicone tube was placed in the axilla at the junction of the nerve root and the dural sac to make an animal model of nerve compression. (For details, see: Liu Jinxian, Li Shangrong. Experimental Research on the Establishment of Rat Lumbar Nerve Root Compression Model. http: / / www.yixue360.com / FileUp / S81 / 116241736121003.html...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The inside diameter ofaaaaaaaaaa
Outer diameteraaaaaaaaaa
Tube chiefaaaaaaaaaa
Login to View More

Abstract

The invention relates to a tube pressing-type chronic nerve root injury model in animal. A method for constructing the model comprises the steps of: wrapping a silicone tube with a C-shaped cross section on the position 2-3mm above the dorsal root ganglion of the nerve root of a rat, wherein the inner diameter of the silicone tube is 0.9-1.1mm, the outer diameter is 1.2-3mm, and the tube length is 2-4mm. The invention further provides the method for constructing the model and the application of the model. The tube pressing-type chronic nerve root injury model in animal provided by the invention has the advantages of simple technology and easy duplication, uniform, objective and standard produced pressure, small individual variation for pathological change, stability, reliability, high repeatability and convenience for comparison, and is closer to the pathological conditions of chronic compression caused on the nerve root by the intervertelbral disc or osteophyte, and a compressed model of nerve root disease which is stable, reliable, easy to standardize and convenient to display effects of treatment factors is provided for operation, medicine, acupuncture and moxibustion and the like in treating chronic nerve root injury.

Description

technical field [0001] The invention relates to an animal model, in particular to a tube pressure chronic nerve root injury animal model and its construction method and application. Background technique [0002] Radiculopathy is a neuropathic pain-based disease caused by mechanical compression or stimulation of nerve roots by inflammatory factors. Herniated intervertebral discs or hypertrophic osteophytes can compress or stimulate nerve roots, resulting in a series of clinical symptoms And physical symptoms. Mechanical compression and chemical stimulation are the two major pathogenic factors that lead to nerve root injury, but the specific mechanism of nerve root injury is not clear, so the establishment of relevant animal models is the first key to solve the problem. Researchers have established different animal models based on these two major pathogenic factors. [0003] 1. Chemical injury model: chemical injury model is a kind of animal model for studying how chemical f...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61D1/00
Inventor 高令军魏佑震
Owner SHANGHAI HOSPITAL OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com