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Method for producing L-threonine

A technology of threonine and isoleucine, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem of no feeding process control method, lack of feeding basis for the control method, and unfavorable feeding. Process real-time control and other issues

Active Publication Date: 2013-09-04
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the above feeding process control technology can improve the production efficiency of amino acid fermentation to a certain extent, there are still the following problems and deficiencies: Some existing technical solutions are strong, although they emphasize that adding certain components will improve the fermentation production efficiency, but there is no Provide a clear feeding process control method; although some existing technical solutions provide some simple feeding process control methods, these control methods lack the basis for feeding and cannot achieve accurate and optimal control; some existing technologies use fermentation condition parameters (such as pH) is the feeding process control basis to carry out batch method feeding, but can't realize continuous accurate control; Part of the prior art uses the concentration of some components (such as sulfate, amino acid) in the fermentation broth as the feeding process However, the determination methods of these components are complex, time-consuming, and poor in operability, which is not conducive to the real-time control of the feeding process

Method used

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  • Method for producing L-threonine
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  • Method for producing L-threonine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1. Fermentation to produce L-threonine (isoleucine feed)

[0063] 1. Experimental materials

[0064] The seed culture medium used in this example is composed of water and solute; the concentration of the solute in the culture medium is as follows: glucose 40g / L, ammonium sulfate 15g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 2g / L, Yeast powder 2g / L, L-isoleucine 0.05g / L, calcium carbonate 15g / L, trace element mixture 2mL / L.

[0065] The fermentation initial medium used in this example is composed of water and solute; the concentration of the solute in the medium is as follows: glucose 10g / L, ammonium sulfate 10g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 2g / L , Yeast powder 2g / L, trace element mixture 2mL / L.

[0066] Through preliminary experiments, it was determined that the cell yield of Escherichia coli EC125 with L-isoleucine as the substrate was 107g / g (that is, 107 grams of Escherichia coli EC125 with a dry weight per gram of L-isole...

Embodiment 2

[0101] Example 2: Fermentation to produce L-threonine (mixed feed of potassium dihydrogen phosphate and isoleucine)

[0102] 1. Experimental materials

[0103] The seed culture medium used in this example is composed of water and solute; the concentration of the solute in the culture medium is as follows: glucose 40g / L, ammonium sulfate 15g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 2g / L, Yeast powder 2g / L, L-isoleucine 0.05g / L, calcium carbonate 15g / L, trace element mixture 2mL / L.

[0104] The initial fermentation medium used in this example is composed of water and solute; the concentration of the solute in the medium is as follows: glucose 10g / L, ammonium sulfate 10g / L, magnesium sulfate 2g / L, yeast powder 2g / L, trace amount Element mixture 2mL / L.

[0105] 2. Fermentation to produce L-threonine

[0106] 1. Same as Step 2 of Example 1.

[0107] 2. Inoculate the seed liquid of step 1 with 3% inoculum (volume ratio) in the fermentation initial medium in the fermentor, an...

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Abstract

The invention discloses a method for producing L-threonine. The method comprises the following steps of: performing fermentation culture on strains capable of producing the L-threonine to obtain the L-threonine, wherein in the fermentation culture process, a period before the growth quantity of thalli is not increased is used as a first stage, and a period in which the growth quantity of the thalli is not increased is used as a second stage; at the first stage, adding nutritional ingredients into a fermentation culture system in an index feed supplement mode on the premise of realizing the optimal production rate of the strains; and at the second stage, adding the nutritional ingredients into the fermentation culture system in a constant speed feed supplement mode on the premise of realizing the maximum production rate of the L-threonine by using the strains. The method has the advantages that: accuracy control and optimizing control are convenient to realize; the operability is high, and the production process is convenient to regulate and control in real time; and the production efficiency can be further improved. The method has a great significance for the industrial production of the L-threonine.

Description

Technical field [0001] The present invention relates to a method for producing L-threonine. Background technique [0002] L-threonine is an essential amino acid, which is widely used in medicine, food, animal feed, etc. In recent years, the demand for L-threonine in domestic and foreign markets has increased year by year. Currently, the main method for producing L-threonine is fermentation. The improvement of fermentation process regulation can increase the production efficiency of amino acids, reduce production costs, and reduce environmental pollution. Therefore, the regulation of the fermentation process has very important practical significance for the industrial production of L-threonine. [0003] There are three main ways to produce L-threonine by fermentation: batch (or batch) fermentation, continuous fermentation and fed-batch fermentation. Fed-batch fermentation can overcome the low production efficiency caused by the osmotic pressure caused by the excessively high ini...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/08C12N1/20C12R1/19
Inventor 温廷益刘树文陈宁梁勇刘茜陶桐桐
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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