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Method for producing L-threonine

A threonine and production rate technology, applied in the field of L-threonine production, can solve problems such as poor operability, lack of feeding basis for control methods, and inability to achieve precise and optimal control.

Active Publication Date: 2012-01-04
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the above feeding process control technology can improve the production efficiency of amino acid fermentation to a certain extent, there are still the following problems and deficiencies: Some existing technical solutions are strong, although they emphasize that adding certain components will improve the fermentation production efficiency, but there is no Provide a clear feeding process control method; although some existing technical solutions provide some simple feeding process control methods, these control methods lack the basis for feeding and cannot achieve accurate and optimal control; some existing technologies use fermentation condition parameters (such as pH) is the feeding process control basis to carry out batch method feeding, but can't realize continuous accurate control; Part of the prior art uses the concentration of some components (such as sulfate, amino acid) in the fermentation broth as the feeding process However, the determination methods of these components are complex, time-consuming, and poor in operability, which is not conducive to the real-time control of the feeding process

Method used

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  • Method for producing L-threonine

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Experimental program
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Effect test

Embodiment 1

[0062] Embodiment 1, production of L-threonine by fermentation (isoleucine feed)

[0063] 1. Experimental materials

[0064] The used seed culture medium of the present embodiment is made up of water and solute; The concentration of described solute in culture medium is as follows: glucose 40g / L, ammonium sulfate 15g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 2g / L, Yeast powder 2g / L, L-isoleucine 0.05g / L, calcium carbonate 15g / L, trace element mixture 2mL / L.

[0065] The initial fermentation medium used in this embodiment is composed of water and solute; the concentration of the solute in the medium is as follows: glucose 10g / L, ammonium sulfate 10g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 2g / L , yeast powder 2g / L, trace element mixture 2mL / L.

[0066] It is determined by preliminary experiments that the cell yield of Escherichia coli EC125 using L-isoleucine as a substrate is 107g / g (that is, every gram of L-isoleucine produces 107 grams of dry...

Embodiment 2

[0101] Embodiment 2, production of L-threonine by fermentation (potassium dihydrogen phosphate and isoleucine mixed feed)

[0102] 1. Experimental materials

[0103] The used seed culture medium of the present embodiment is made up of water and solute; The concentration of described solute in culture medium is as follows: glucose 40g / L, ammonium sulfate 15g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 2g / L, Yeast powder 2g / L, L-isoleucine 0.05g / L, calcium carbonate 15g / L, trace element mixture 2mL / L.

[0104] The initial fermentation medium used in this embodiment is composed of water and solute; the concentration of the solute in the medium is as follows: glucose 10g / L, ammonium sulfate 10g / L, magnesium sulfate 2g / L, yeast powder 2g / L, trace Element mixture 2mL / L.

[0105] 2. Production of L-threonine by fermentation

[0106] 1. Same as step 2 of embodiment 1.

[0107]2. Inoculate the initial fermentation medium in the fermenter with the seed liquid of step 1...

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Abstract

The invention discloses a method for producing L-threonine. The method comprises the following steps of: performing fermentation culture on strains capable of producing the L-threonine to obtain the L-threonine, wherein in the fermentation culture process, a period before the growth quantity of thalli is not increased is used as a first stage, and a period in which the growth quantity of the thalli is not increased is used as a second stage; at the first stage, adding nutritional ingredients into a fermentation culture system in an index feed supplement mode on the premise of realizing the optimal production rate of the strains; and at the second stage, adding the nutritional ingredients into the fermentation culture system in a constant speed feed supplement mode on the premise of realizing the maximum production rate of the L-threonine by using the strains. The method has the advantages that: accuracy control and optimizing control are convenient to realize; the operability is high,and the production process is convenient to regulate and control in real time; and the production efficiency can be further improved. The method has a great significance for the industrial productionof the L-threonine.

Description

technical field [0001] The present invention relates to a method for producing L-threonine. Background technique [0002] L-threonine is an essential amino acid, widely used in medicine, food, animal feed, etc. In recent years, the demand for L-threonine in domestic and foreign markets has increased year by year. At present, the main method for producing L-threonine is fermentation. The improvement of fermentation process regulation can improve the production efficiency of amino acids, reduce production costs and reduce environmental pollution. Therefore, the regulation of the fermentation process has very important practical significance for the industrial production of L-threonine. [0003] There are three main ways to produce L-threonine by fermentation: batch (or batch) fermentation, continuous fermentation and fed-batch fermentation. Fed-batch fermentation can overcome the low production efficiency caused by osmotic pressure or improper composition ratio caused by h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/08C12N1/20C12R1/19
Inventor 刘树文温廷益陈宁梁勇刘茜陶桐桐
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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