Method for preparing conjugated linoleic acid rich bean pulp feed by secondary raw material fermentation

A technology of linoleic acid soybean meal and secondary meal, which is applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the problems of high production cost, no wine and sour aroma, poor aroma and taste of fermented soybean meal feed, etc. Good palatability and obvious food-attracting effect

Inactive Publication Date: 2012-01-18
ANHUI AGRICULTURAL UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

Its shortcoming is that, due to the use of clinker fermentation, the production cost is high, and it does not have the unique wine acid aroma of yeast fermentation, the produced fermented soybean meal feed has poor aroma and taste, so the amount added in the feed restricted

Method used

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Experimental program
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Embodiment Construction

[0013] 1. Preparation of seed liquid: Inoculate Candida utilis ANY01 (Candida utilis ANY01) into PDA solid slant medium (components are shown in Table 1), place in a 30°C constant temperature incubator for cultivation, plant lactic acid bacteria ANCLA01 ( Lactobacillus plantarum ANCLA01) was inoculated into MRS solid slant medium (the components are shown in Table 2), cultured in a 37°C constant temperature incubator, and continuously activated for 2 times. Cultivate in an incubator at °C and 37°C, prepare the primary seed liquid, and then inoculate them in PDA and MRS liquid medium with 10% inoculum, and culture in an incubator at 30°C and 37°C to prepare the secondary seed liquid. Concentration of the secondary seed solution of Candida utilis ANY01 ≥ 2.40×10 8 cfu / mL, the secondary seed liquid concentration of plant lactic acid bacteria ANCLA01 is 2.00×10 8 cfu / mL.

[0014] Table 1: Components of PDA Medium

[0015] Potato

200g

glucose

20g

...

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Abstract

The invention discloses a method for preparing conjugated linoleic acid rich bean pulp feed by secondary raw material fermentation. The method comprises the following steps of: (1) respectively preparing fermentable seed liquor from candida utilis ANY01 and lactobacillus plantarum ANCLA01 by slant solid culture, two-time continuous activation, shake flask liquor culture, two-time continuous activation and expanded culture; and (2) crushing bean pulp, sieving the crushed bean pulp, adding bran, phosphor salt, potassium salt, magnesium salt and deionized water into the bean pulp to inoculate the candida utilis ANY01, regulating the pH, controlling the temperature, fermenting, adding bran, phosphor salt, potassium salt, magnesium salt, sodium chloride, trace manganese, vegetable fat and deionized water to inoculate the lactobacillus plantarum ANCLA01, regulating the pH, controlling the temperature and fermenting. The conjugated linoleic acid rich bean pulp feed is prepared by adopting the secondary raw material fermentation method; after secondary fermentation, the total protein content of the bean pulp feed is improved by about 3 percent; and the bean pulp feed has thick vinic acid aroma and good palatability, and has obvious attraction for livestock and poultry.

Description

[technical field] [0001] The invention relates to the field of animal nutrition and feed biotechnology, in particular to a method for preparing soybean meal feed rich in conjugated linoleic acid by secondary raw meal fermentation. [Background technique] [0002] Conjugated linoleic acid (CLA) is a group of linoleic acid isomers derived from linoleic acid. It is a nutrient commonly found in humans and animals. It can inhibit fat deposition, improve meat quality and resist oxidation. , Prevent arteriosclerosis and anti-cancer, improve the body's immunity, promote animal growth and other positive functions. A large number of research experiments have proved that conjugated linoleic acid is neither toxic nor has any side effects. CLA has become a research hotspot in current natural health products and feed additives. Natural conjugated linoleic acid mainly exists in ruminants, such as milk fat and meat products of cattle and sheep, but the amount is small, and CLA for feed addi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K1/16A23K1/175A23K10/12A23K10/37A23K20/26
CPCY02P60/87
Inventor 王力生方飞章孝荣程茂基程建波丁建平蔡海莹刘洪瑜周韬张子军丁小玲
Owner ANHUI AGRICULTURAL UNIVERSITY
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