132 results about "Eucalyptus citriodora" patented technology

The invention discloses a perfumed mosquito repellent composition, characterized in that the composition is prepared by the following ingredients, based on percent by weight, of: 0.1-1% of wild lemonleaf oil, 0.1-2% of eucalyptus oil, 0.5-3% of tea tree oil, 1-10% of eucalyptus citriodora leaf oil, 0-0.5% of clove oil, 0-0.2% of linaloe wood oil, 0-0.5% of dementholised peppermint oil and the balance as diluent of alkane C14. The mosquito repellent composition makes full use of three mosquito repellent essential oils including eucalyptus oil, tea tree oil and eucalyptus citriodora leaf oil, which all have no pesticide ingredients contained, but are excellent in repelling mosquito, bugs and ants, thus achieving ideal mosquito repellent effect and acceptance for human body and generating noside effect to human body, besides, the cost of the composition is low.

The invention discloses compound plant essential oil with effects of repelling mosquitos, relieve itching and subsiding swellings. The compound plant essential oil comprises the following raw materials: artemisia vulgaris oil, lemon essential oil, eucalyptus citriodora oil, clove essential oil, camellia seed essential oil, catnip essential oil, thyme essential oil, geranium essential oil, rosemaryessential oil, castor oil, citronella oil, litsea oil, lavender essential oil, lithospermum essential oil, clary sage essential oil, mentha spicata essential oil, cypress essential oil, platycladus orientalis essential oil, calendula officinalis essential oil, cedarwood essential oil, juniper berry essential oil, wild chrysanthemum essential oil, leonurus japonicus essential oil, eucommia ulmoides essential oil, jasmine essential oil, eucalyptus essential oil, valeriana officinalis essential oil, rose essential oil, osmanthus fragrans essential oil and lemongrass oil. The compound plant essential oil has obvious mosquito repelling effect, high itching relieving and swelling subsiding speeds, remarkable improvement effects on eczema improving suppuration, herpes, pimples, swelling furuncleand itching skin as well as obvious anti-bacterial and anti-inflammation functions.

Concentrated vapors from botanical essential oils are inhaled to prevent, treat and cure infections of the respiratory pathogens causing Severe Acute Respiratory Syndrome (“SARS”). These vapors are inhaled as a practical method to reduce the risks of infection by the pathogens causing SARS in crowded public places. These vapors are also inhaled as a practical method to reduce the risks of infection by unknown, and unpredictable, respiratory pathogens that may be present in public places. The essential oils have antiseptic properties, are safe to inhale, and include, but are not limited to, the essential oils from Eucalyptus globulus, Melaleuca alternifolia, Eucalyptus citriodora, and Eucalyptus radiata. Convenient hand-held inhaler apparatus are provided for the inhalation of concentrated vapors from the antiseptic essential oils and other substances. The antiseptic essential oils have selected antiviral, antibacterial, and antifungal properties.

The invention discloses an ecological disinfestant and preparing method thereof. The ecological disinfestant comprises the following raw materials of: ferment, camphor bark, melangolo leaf, cannabis sativa leaf, angled bittersweet, pyrethrum, folium artemisiae argyi, Eucalyptus citriodora leaf and allicin. The ecological disinfestant is prepared by the steps, such as crushing, solvent extraction, recovery, filtering, concentrating, drying, mixing and pelleting. The ecological disinfestant is high in disinsection antibacterial property, crop pests are smudged via the bitter and sour smells of multiple raw materials to reduce the using amount of the insecticide; and particularly, for crops, such as tea trees, pesticide residues on tea leaves can be avoided, the photosynthesis of laminae can be strengthened, nutrient absorption is promoted, and thereby improving the quality of tea leaves further.

The invention relates to natural plant extracts, particularly relates to a mixture of natural plant extracts and a preparation method and application of the mixture and particularly discloses extracts of lemon eucalyptus, rosemary, liquorice, cinnamon and hyperforin perforatum and application of extracts in preparation of a disinfecting agent. Natural edible plant constituents serve as effective antibiotic constituents, other natural food additive constituents such as citric acid and sodium citrate are added to prepare the pure natural plant type disinfecting agent which is safe and nontoxic, and the disinfecting agent has the good preservation effect.

The invention discloses a method for extracting eucalyptus citriodra volatile oil and 3,8-diol, which is characterized by comprising the following steps: 1) cutting fresh leaves of eucalyptus citriodora into fragments; 2) pilling the leaves of the eucalyptus citriodora for fermentation; 3) extracting a volatile oil of an eucalyptus citriodra oil phase by distillation by a sodium chloride-containing steam distillation method from the cut and fermented leaf fragments of the eucalyptus citriodora, which serve as the raw material; 4) extracting raffinate, which is obtained after the steam distillation, by using a solvent; 5) drying the solvent, which is obtained after extraction, with anhydrous sodium sulfate; and 6) reclaiming the solvent to obtain a yellowish oily material which is the 3,8-diol. In the method, the extraction rates and content of the eucalyptus citriodra volatile oil and the 3,8-diol are improved by combining fermentation technology and steam distillation, the content of the obtained 3,8-diol accounts for 80 percent of water soluble components and meets the 60 percent content requirement on mosquito-repelling products overseas, the 3,8-diol can be sold directly, and thus the comprehensive utilization value of eucalyptus citriodora is improved.

The invention provides health pillows for treating common cold, cephalalgia, nervous prostration, resisting fatigue and alleviating mental stress, which is prepared from hierochloe odorata, cordate houttuynia, forest gray gum, eucalyptus citriodora, lysimachia christinae and swamp mahogany through drying and chopping.

The invention discloses a plant essential oil composition and an application thereof, and relates to the field of mosquito repelling. The plant essential oil composition disclosed by the invention comprises 10 to 30 parts of carapa guaianensis seed oil, 15 to 40 parts of eucalyptus citriodora oil, 5 to 25 parts of thyme essential oil, 10 to 30 parts of yarrow essential oil and 10 to 30 parts of geranium essential oil. The plant essential oil composition is natural and non-irritating, can effectively repel mosquitos and mites when being applied to spray, has relatively good antibacterial and itching-relieving effects, is safe and non-irritating to the skin, and has good temperature resistance.

The invention provides a natural plant abluent applicable to bowls and chopsticks characterized by taking three nontoxic and harmless pure natural plants as main raw materials such as cylindrical luffa leaf, hybrid paper mulberry leaf and wide-leaf aloe saponaria leaf (or aloe vera lea and shang-nong large-leaf aloe leaf), wherein the proportion of the main raw materials to the finished product is 56.00%-84.00%. A prescription A directly adopts the primary pulp of the three leaf abluent; and a prescription B directly adopts the former two leaf abluent and the later leaf abluent. Due to the physical characteristics of the three plants, the other eight auxiliary materials are added according to a certain sequence and proportion: drinking pure water, tea saponin, soapberry extracting solution, salt, edible glacial acetic acid, nisin, cinnamyl formate and lemon eucalyptus oil to physically prepare the natural plant cleaning product which can not injure the skin, can be completely and fastbiologically degraded, is free of toxicity and residue, can avoid the secondary water body pollution, is environment-friendly, economical, and suitable for cleaning the tableware, the vegetable, the fruit or the human body surface such as the hand, the foot and the like.

The invention relates to an environmentally-friendly phytochemical pasty biopesticide and a preparation method thereof. The biopesticide is characterized by being prepared by grinding and boiling the following raw materials in percentage by mass in water: chinaberry seeds 25-45, eucalyptus citriodora branches and leaves 0-20, croton fruit 5-15, star anise 0-10, fennel 5-10, tea seed cake 20-30 and colza cake 5-10, wherein the total mass percentage of the raw material components is 100 percent. The biopesticide is suitable for preventing and controlling pest and disease damages to woody fruit trees of longan, lichee, mango, mandarine, persimmon, apple, snow pear, pomelo and the like and is applied on tree trunks to repel and contact and kill pests. And the biopesticide can obviously improve the photosynthesis of the leaf surfaces, promote the sound growth of the fruit trees, increase the strength of flower stalks and the thickness of petals when applied before blossom, improve fruit setting rates when applied in young fruit period, make fruit avoid drop, ripe fruits even, ripe period advanced or hanging period prolonged when applied in middle fruit period, and lay a basis for shooting promotion and high yield in the coming year when applied after harvest.

Anti-microbial toilet soap and a preparation method thereof. The anti-microbial toilet soap includes following raw materials, by mass, 190-280 parts of a soap base, 50-55 parts of eucalyptus citriodora leaves, 20-24 parts of radix glycyrrhizae, 15-18 parts of cortex phellodendri, 11-14 parts of mulberry leaves and 10-20 parts of andrographis paniculata. In the invention, the eucalyptus citriodora leaves, the radix glycyrrhizae, the cortex phellodendri, the mulberry leaves and the andrographis paniculata are compounded reasonably. The raw materials are natural and environmental-protective. The toiled soap has an excellent anti-microbial property.

The invention relates to a mosquito-repelling, itching-relieving and bacteriostatic plant essential oil spray and an application. The mosquito-repelling, itching-relieving and bacteriostatic plant essential oil spray is prepared from the following raw material components in parts by weight: 96-98 parts of water, 1.5-3 parts of solubilizers and 0.58-1.25 parts of composite plant antibacterial essential oil, wherein the composite plant bacteriostatic essential oil is prepared from cymbopogon citratus leaf oil, lavender oil, pelargonium graveolens flower oil and lemon eucalyptus oil. The mosquito-repelling, itching-relieving and bacteriostatic plant essential oil spray has the beneficial effects that a composite plant bacteriostatic essential oil component formed by compounding four natural plant essential oils is added to the mosquito-repelling, itching-relieving and bacteriostatic plant essential oil spray, so that the mosquito-repelling, itching-relieving and bacteriostatic plant essential oil spray integrates multiple effects of repelling mosquitoes, relieving itching, resisting inflammation, repairing, inhibiting bacteria and the like, is fresh and soft in smell, does not irritate respiratory tracts, is free of skin irritation,; and can be used by infants and pregnant and lying-in women.

The invention belongs to the technical field of mosquito repellent products and particularly discloses a herbal plant mosquito repellent spray liquid and a preparationmethod thereof. The mosquito repellent spray liquid is prepared from the following components in percentage by weight: 14%-20% of traditional Chinese medicine, 1%-3% of traditional Chinese medicine essential oil, 70%-77% of 30%(v / v) ethyl alcohol and 5%-10% of glycerine, wherein the traditional Chinese medicine comprises illicium verum, cumin, Sichuan peppercorn, bay leaves, cortex cinnamomi, mint,flos caryophylli, folium artemisiae argyi, Chinese ilex leaves, cacumen platycladi, herba artemisiae annuae, rhizoma atractylodis, herba schizonepetae, litsea cubeba and lemongrass herbs; the traditional Chinese medicine essential oil is a mixture of rosemary oil and eucalyptus citriodora oil. The mosquito repellent spray liquid is safe, environment friendly, clean, pollution-free and harmless to human, and has better mosquito repellent effect.

The invention belongs to the field of antibacterial preservatives and food preserving packaging materials or food processing mechanical coatings and particularly relates to preparation and application of Eucalyptus citriodora oil and g-C3N4 composite modified waterborne polyurethane antibacterial coating. By adsorbing Eucalyptus citriodora oil to g-C3N4 composite, volatilization of Eucalyptus citriodora oil in use is reduced, waste of Eucalyptus citriodora oil is reduced, utilization rate of the Eucalyptus citriodora oil is increased, and long-acting antibiosis and efficient utilization are achieved; the composite is then used to modify waterborne polyurethane antibacterial coating so that stability and antibacterial and mildew-proof properties of the coating are improved.

The invention discloses a super-clean sterilizing liquid for a washing machine. The super-clean sterilizing liquid comprises the following raw materials in parts by weight: 3-8 parts by weight of polyhexamethylene biguanide hydrochloride, 3-10 parts by weight of an eucalyptus citriodora essential oil extract, 5-30 parts by weight of an organic poly-phosphate acid, 45-80 parts by weight of a surfactant, 5-15 parts by weight of a solubilizer, 3-15 parts by weight of a chelating agent and 0.1-2 parts by weight of an aromatic solvent. The invention also provides a preparation method of the super-clean sterilizing liquid for the washing machine, and the super-clean sterilizing liquid prepared by the method can have a killing effect of 99.9% on the bacteria of escherichia coli, staphylococcus aureus, candida albicans, F's dysentery bacillus, pseudomonas aeruginosa and the like, and has a good antibacterial effect (according to the following standard: a product with a bacteriostatic rate fromequal to or higher than 50% to lower than 90% has a bacteriostatic effect, and a product with a bacteriostatic rate of equal to or higher than 90% has a strong bacteriostatic effect). The bacteriostatic rate of the super-clean sterilizing liquid for the washing machine in the embodiment reaches 90%, and has an excellent disinfection and sterilization effect.

The invention relates to mosquito-repelling sustained-release dropping pills and a preparation method.The mosquito-repelling sustained-release dropping pills are characterized by be prepared from an essential oil combination and a matrix, wherein the weight ratio of the essential oil combination to the substrate ranges from 1:1 to 1:8, and the essential oil combination is prepared from eucalyptus citriodora essential oil, basil sweet essential oil, vanilla oil, jasmine essential oil and synergist.The preparation method comprises the steps that the eucalyptus citriodora essential oil is melted, the basil sweet essential oil, the vanilla oil, the jasmine essential oil and the synergist are added, and the materials are stirred to be uniform; the mixture is added to the melted matrix and stirred to be uniform, and the constant temperature is maintained; the dropping pills are preparedin a pill dropping machine according to a specified technology.According to the mosquito-repelling sustained-release dropping pills and the preparation method, the usage quantity and order of use of the pills can be self-assembled according to different usage requirements and hobbies, and therefore single active ingredient slow-release quantity and slow-release duration of the essential oil are controlled, mosquitoes cannot produce insecticide resistance, the pills can be matched with multiple mosquito-repelling carriers for use, and the mosquito repelling effect is good.

The invention discloses an environmentally-friendly botanic biological pesticide. The pesticide is characterized in that the pesticide comprises, by weight, 20-30 parts of Chinaberry seed, 10-20 parts of Eucalyptus citriodora branch and leaf, 5-10 parts of Croton Seed, 10-25 parts of Illicium verum, 5-12 parts of anise, 20-26 parts of tea seed cake, 10-16 parts of rapeseed cake, 1-10 parts of a synergist, 5-20 parts of Herba Taraxaci, 4-10 parts of purslane, 6-9 parts of Siberian Cocklour Fruit, 10-15 parts of pomegranate rind, 11-15 parts of Artemisia argyi, 12-18 parts of Herba Houttuyniae, 5-18 parts of Andrographis paniculata and 100-150 parts of water. The environmentally-friendly pesticide is a novel pesticide having the advantages of high effectiveness, pertinence, environmental protection, no secondary toxicity, no residual, no pollution, ecologic balance maintenance and no nuisance, is an important guarantee for realizing the ecologic agriculture, the green ecologic agriculture and green enterprises, and has a broad prospect.

The invention discloses an environment-friendly disinfectant which includes, by mass, 10-12 parts of muskmelon pedicel, 2-4 parts of oat glucan, 7-8 parts of scutellaria baicalensis, 6-8 parts of morinda officinalis, 2-4 parts of eucalyptus citriodora gum, 4-6 parts of glacial acetic acid, 3-5 parts of radix scrophulariae, 6-7 parts of clinopodium polycephalum, 4-6 parts of herba aristolochiae, 5-7 parts of cape jasmine, 7-8 parts of concha arcae, and 5-7 parts of fructus teminaliae billaricae. The disinfectant is prepared by finely extracting traditional Chinese medicines, so that the disinfectant ensures sterilization and cleaning functions and also avoids secondary pollution on environment since the raw materials are all natural traditional Chinese medicines.

The invention relates to a textile anti-mosquito finishing agent. The textile anti-mosquito finishing agent is prepared from the following components in parts by weight: 1 to 3 parts of ethyl N-acetyl-N-butyl, 1 to 2 parts of menthol, 2 to 4 parts of linalool, 1 to 2 parts of eucalyptol, 3 to 6 parts of thuja sutchuenensis extract, 4 to 7 parts of eucalyptus citriodora extract, 8 to 12 parts of Chinese herb extract and 0.5 to 1.5 parts of povidone-iodine. The invention also relates to a preparation method of the textile anti-mosquito finishing agent. The prepared textile anti-mosquito finishing agent can be used for finishing a textile by utilizing a conventional finishing method. The finished textile can prevent mosquitoes from approaching the human body, can be used for finishing textiles such as clothes and tents for outdoor climbing and outing, clothes for fishing, curtains, towel blankets and pajamas, clothes and gloves for gardening, working suits for agricultural production andmilitary clothes, and can effectively prevent the diseases caused by the propagation of mosquitoes.

The invention discloses a natural plant mosquito repellent. The natural plant mosquito repellent is prepared from raw materials in parts by mass as follows: 40-60 parts of edible ethanol, 1-10 parts of dementholized peppermint oil, 1-6 parts of citronella oil, 3-12 parts of wintergreen oil, 3-12 parts of eucalyptus citriodora oil, 3-12 parts of blumea oil, 3-12 parts of lavender oil, 8-16 parts ofsodium lauryl sulfate, 35-45 parts of sodium chloride, 4-9 parts of glycine betaine, 3-10 parts of honeysuckle extract and 3-5 parts of acid or acidic compounds; total pH value of a mosquito repellent solution is regulated to 4-4.5, the mosquito repellent solution is left to stand for 1-1.5 h, solid-liquid separation is performed after the mosquito repellent precipitates, and the liquid-state mosquito repellent is obtained after solid precipitates are removed. The natural plant mosquito repellent has technical benefits as follows: the mosquito repellent is prepared from the plant extracts asraw materials through scientific refining and is free of toxic and side effects and stimulation on a human body, and effective time for repelling mosquito bites is as long as six hours. Due to correctsetting of the pH value, aroma becomes mellow and normal and non-irritant, even people with nasal sensitivity cannot be troubled, and the application range of the natural plant mosquito repellent isexpanded.

The invention discloses a green environment-friendly pesticide preparation which is characterized by being composed of the following components in parts by weight: 1-3 parts of candida powder, 0.1-0.3 part of Bacillus thuringiensis powder, 1-3 parts of Oceanospirillales powder, 9-16 parts of neem seed, 8-13 parts of eucalyptus citriodora branch and leaf, 5-9 parts of Macleaya cordata, 7-10 parts of cabo, 9-10 parts of gynura segetum, 2-6 parts of azedarach, 10-20 parts of creeping oxalis, 8-10 parts of nux vomica, 8-10 parts of tea seed cake, 2-9 parts of grapeleaf anemone, 2-9 parts of yellow sandalwood, 10-20 parts of matrine, 12-32 parts of nicotine, 18-24 parts of cynanchum komarovii, 3-9 parts of cynanchum komarovii, 8-9 parts of triperygium wilfordii and 100 parts of water. The green environment-friendly pesticide preparation disclosed by the invention is a novel pesticide which has high efficiency, pertinence, environment friendliness, no secondary toxicity, no residue and no pollution, keeps ecological balance and is nuisance-free, is an important guarantee for realizing ecological agriculture, green ecological agriculture and green enterprises, and has wide prospects.

The present invention provides mosquito-repelling and itch-stopping compound essential oil, a preparation method and an application thereof, and a mosquito-repelling and itch-stopping product, and relates to the technical field of essential oils. The mosquito-repelling and itch-stopping compound essential oil comprises the following components in parts by mass: 0.5-2 parts of cymbopogon citratus sesame oil, 0.3-1.5 parts of eucalyptus citriodora leaf oil, 0.1-1 part of rosemary essential oil, 0.1-1 part of basil essential oil, 0.08-0.8 part of santolina chamaecyparissus essential oil, 0.5-1.5parts of elsholtzia ciliata essential oil, 0.1-1 part of fructus amomi rotundus essential oil and 0.08-0.8 part of pelargonium hortorum essential oil. The mosquito-repelling and itch-stopping compoundessential oil solves problems that traditional mosquito-repelling products are relatively large in toxicity, pollute environment and affect body health, mosquito-repelling and itch-stopping productshave certain irritancy and toxicity, and single mosquito-repelling essential oil is single in effects and not high in mosquito-repelling efficiency. The provided mosquito-repelling and itch-stopping compound essential oil has advantages of being green, healthy, free of pollution, low in toxicity, free of irritation, obvious in mosquito-repelling and itch-stopping effects, etc., and at the same time is simple to manufacture, convenient to use and good in application prospects.

The invention discloses natural liquid as well as a preparation method and application thereof, and relates to mosquito killing liquid. The natural liquid is prepared from the following raw materials of p-menthane-3,8-diol, citriodora oil or citronellal and a catalyst, wherein the mass ratio of the p-menthane-3,8-diol to the citriodora oil or citronellal is 1:(0.5 to 2); the adding amount of the catalyst is 0.1 to 2 percent of the p-menthane-3,8-diol according to the mass percentage. The citriodora oil or citronellal and the catalyst are added into the p-menthane-3,8-diol, so that after dewatering reaction, the reaction liquid is purified, and the natural liquid is obtained. The natural liquid can be applied to mosquito incense liquor; the content of the natural liquid in the mosquito incense liquor is 2 to 10wt%, and the balance is a solvent; after 30 minutes, the mosquito knockdown rate is not less than 95 percent, the avoidance rate is not greater than 5 percent, and the total mosquito expelling rate is up to 100 percent. The natural liquid is directly separated and extracted from eucalyptus citriodora oil, so that waste of a large quantity of eucalyptus citriodora raw materials can be avoided, the cost can be reduced, and the yield can be increased.

The invention provides plant essence itching-relieving mosquito-repellent liquid and a manufacturing method thereof. The method includes steps: adding, by weight, 5-10 parts of clove oil and 5-10 parts of eucalyptus citriodora oil into 30-50 parts of ethanol water solution to obtain first solution; adding, by weight, 10-15 parts of fructus cnidii, 10-15 parts of fructus kochiae and 10-20 parts of folium artemisiae argyi into 120 parts of ethanol water solution, and performing reflux extraction for 3-4h at a constant temperature of 30-40 DEG C and under a pressure of 0.1-0.13MPa to obtain second solution; concentrating the second solution to 40-50 parts by weight, and mixing with the first solution; adding a ph value regulator to regulate a ph value to 6.5. The plant essence itching-relieving mosquito-repellent liquid has functions of itching relieving and mosquito repelling.

The invention discloses a green pesticide containing microorganisms, which is composed of the following components in parts by weight: 1-3 parts of monad powder, 0.1-0.2 part of Beauveria bassiana powder, 0.3-0.5 part of Metarrhizium anisopliae powder, 1-3 parts of Oceanospirillales powder, 9-16 parts of neem seed, 8-13 parts of eucalyptus citriodora branch and leaf, 5-9 parts of Macleaya cordata, 7-10 parts of cabo, 9-10 parts of gynura segetum, 2-6 parts of azedarach, 10-20 parts of creeping oxalis, 8-10 parts of nux vomica, 8-10 parts of tea seed cake, 2-9 parts of grapeleaf anemone, 2-9 parts of yellow sandalwood, 5-10 parts of sapogenin and 100 parts of water. The biological environment-friendly pesticide disclosed by the invention is a novel pesticide which has high efficiency, pertinence, environment friendliness, no secondary toxicity, no residue and no pollution, keeps ecological balance and is nuisance-free, is an important guarantee for realizing ecological agriculture, green ecological agriculture and green enterprises, and has wide prospects.

The invention relates to the technical field of research and development of pesticide preparations, in particular to a plant-sourced mosquito-repellent incense and a preparation method thereof. The plant-sourced mosquito-repellent incense is prepared from the following raw materials in parts by weight: 50-60 parts of datura stramonium l, 20-25 parts of matthiola incana, 30-50 parts of herba rosmarini officinalis, 30-40 parts of pelargonium hortorum, 15-30 parts of radix glycyrrhizae, 15-20 parts of herba cymbopogonis citrati, 15-30 parts of pistacia weinmannifolia, 8-15 parts of herba lobeliae chinensis, 8-15 parts of acorus calamus linn, 8-15 parts of herba mirabilis jalapae and 5-10 parts of eucalyptus citriodora. The plant-sourced mosquito-repellent incense provided by the invention is prepared from pure natural traditional Chinese medicinal raw materials at a scientific ratio, is reasonable in collocation of the traditional Chinese medicinal raw materials, is synergistic in effect of the raw materials, free of chemical components, smoke-free and dust-free during use, good in mosquito-repellent effect, harmless to the human body, free of antibacterial agents, preservatives and other chemical additives, environment-friendly, healthy, simple and convenient in use method, and low in cost, and has a certain inhibiting and killing effect to viruses and bacteria of epidemic infectious diseases.

The invention belongs to the field of food antibacterial and fresh-keeping materials, and particularly relates to a eucalyptus citriodora essential oil / nisin nano-emulsion and a preparation method thereof. The preparation method comprises the following steps: dissolving a certain mass of nisin in deionized water, adding a composite emulsifier composed of Tween 80 and soybean lecithin to form an aqueous phase, magnetically stirring, dropwise adding eucalyptus citriodora essential oil-loaded corn oil as an oil phase, and performing stirring homogenization and ultrasonication to form an oil-in-water type nano-emulsion. The nano-emulsion prepared by the invention can effectively improve the water solubility and biocompatibility of the eucalyptus citriodora essential oil, and can achieve the purposes of efficient utilization and long-acting antibacterial effect by virtue of the synergistic effect with the nisin, thereby expanding the application of the eucalyptus citriodora essential oil and the nisin in the field of food antibacterial and fresh-keeping materials.