Method for identifying Bacillus subtilis for producing lipopeptide surfactants

Abstract

The invention discloses a method for identifying Bacillus subtilis for producing lipopeptide surfactants, which comprises the following steps: (1) determining the strain to be identified as a candidate positive strain; (2) determining the positive strain as a candidate lipopeptide-producing strain; and (3) determining the lipopeptide-producing strain as candidate lipopeptide-producing Bacillus subtilis. The method for identifying Bacillus subtilis for producing lipopeptide surfactants can quickly and accurately separate and identify lipopeptide-producing strains, has the advantages of high sensitivity and resolution, convenient operation process, low time consumption and low cost, and can be widely used for the screening and identification of lipopeptide-producing strains.

Description

technical field [0001] The invention relates to a method for identifying Bacillus subtilis producing lipopeptide surfactants. Background technique [0002] Lipopeptide, also known as acylpeptide, is a small peptide composed of a hydrophilic peptide bond and a lipophilic aliphatic chain. Due to its special chemical composition and amphiphilic molecular structure, lipopeptides Biosurfactants have shown very good characteristics and are widely used in fields such as medicine, food, cosmetics, environmental governance and microbial oil recovery [Singh P, Cameotra S S. Potential applications of microbial surfactants in biomedical sciences.Trends in Biotechnology , 2004, 22(3): 142-146]. Most of the lipopeptides are derived from microorganisms; most of them are derived from bacteria. At present, there are more than ten kinds of lipopeptide biosurfactants, mainly including Surfactin, Lichenysin, Iturin, Fengysin, etc. 11-16]. Among them, Surfactin (surfactin) is a kind of lipop...

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Problems solved by technology

[0003] In recent years, people have been using the blood plate method and the oil expansion circle method to screen the strains producing biosurfactants, but this is not only inefficient, but also cannot effectively distinguish lipopeptide-producing microorganisms from them; while identifying the lipopeptides produced by microorganisms The methods generally adopt high performance liquid chromatography [HPLC], nuclear magnetic resonance [NMR], and mass spectrometry to analyze the purified metabolites. These detection methods have high requirements on the purity of metabolites, but the purification of lipopeptide biosurfactants requires After acidification precipitation, freeze-drying, vacuum drying and other cumbersome processes, it is time-consuming and inefficient. Therefore, how to quickly screen the microorganisms that produce lipopeptide surfactants has always been the main problem that plagues researchers.

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