Method for culturing microbes through fermentation

A fermentation culture and microbial technology, applied in the direction of bacteria, etc., can solve the problems of low number of viable bacteria in bacterial powder, low fermentation coefficient, and low bacterial concentration, and achieve the effects of reducing medical burden, high fermentation level, and high spore conversion rate

Active Publication Date: 2012-02-01
远大生命科学(重庆)有限公司
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  • Abstract
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Problems solved by technology

[0007] The present invention aims at the deficiencies in the prior art that microbial fermentation mainly adopts the traditional batch low-density fermentation method, such as low concentration of bacteria in the fermentation broth, low number of live bacteria in the bacterial powder, low fermentation coefficient, low output, high energy consump

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Examples

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Example Embodiment

[0028] The method for fermentation and cultivation of microorganisms of the present invention includes the following process steps:

[0029] 1. According to the process requirements, select suitable bacillus to produce seeds, and use conventional separation and purification treatment to activate and revive the bacteria;

[0030] 2. Primary seed culture: inoculate the activated Bacillus strains into the primary seed liquid medium and cultivate for 6-24 hours at 30-40°C;

[0031] 3. Heat treatment of the first-level seed liquid: heat-treat the first-level seed liquid obtained in step 2: the treatment temperature is 60-90°C, and the time is 5-60 minutes;

[0032] The effect of heat treatment increases the synchronization of microbial growth and improves the adaptability of microorganisms to higher temperatures. The selected strains are more resistant to high temperatures, and strains that cannot adapt to high temperatures are eliminated;

[0033] 4. Second-level seed culture: P...

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Abstract

The invention discloses a method for culturing microbes through fermentation. The method comprises the following process steps of: performing one-stage seed culture on proper bacillus produced seeds, and performing heat treatment at the temperature of between 60 and 90DEG C for 5 to 60 minutes; performing two-stage seed culture and heat treatment; performing three-stage seed culture, heat treatment and cold treatment; inoculating three-stage seeds subjected to cold treatment into a liquid medium, and culturing in a fermentation tank for 16 to 36 hours; and dynamically detecting real-time data in the fermentation culture process and supplying a liquid medium and/or alkali to the fermentation tank in real time. By the method, the viable count and the fermentation coefficient of fermentation liquor are greatly improved, the yield is high, the energy consumption is low, the cost is low, the product quality standard is improved, after the seeds are subjected to heat treatment and cold treatment, the microbes synchronously grow in the fermentation culture process, the obtained microbe powder has high viable count, spores are quickly matured, the spore conversion rate is high, the fermentation level is high, the yield of viable bacteria is high, and the survival rate of the viable bacteria is high.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms, in particular to a method for cultivating bacillus. [0002] Background technique [0003] In the field of microorganisms, it often involves the cultivation and proliferation of strains, so that the strains can be multiplied in a suitable medium under certain temperature conditions to expand the number, so as to meet the requirements of the strains in the fields of human medicine, veterinary medicine, feed, breeding, etc. use needs. Especially for Bacillus, such as Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Clostridium butyricum, etc., it is necessary to ferment and proliferate in the fermenter to significantly increase the number of viable bacteria. [0004] According to the microbial life cycle theory, the microbial growth process can be divided into: adaptation period, logarithmic growth period, equilibrium period, and death period. After the equilibrium period, d...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 江苏华尚玉新
Owner 远大生命科学(重庆)有限公司
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