Method for reducing content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime

An Antarctic krill and quicklime technology, applied in application, food preparation, food science and other directions, can solve the problems of high price, inconvenient operation, reducing the nutritional function of fluorine content, etc., and achieve high intake, low cost and low price. Effect

Inactive Publication Date: 2012-02-08
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, many defluoridation methods and tools are mostly expensive or inconvenient to operate, and Antarctic krill enzymatic hydrolyzate contains a lot of nutrients and active substances. It is required not

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Antarctic krill was mashed, then added distilled water to homogenate to obtain Antarctic krill shrimp paste, and then added Alcalase protease (measured enzyme activity 109387U / g, purchased from Novozyme (China) Biotechnology Co., Ltd.), enzyme The amount is 3010U / g, enzymatic hydrolysis at 50°C and pH value of 8.0 for 240min, then inactivating the enzyme in a water bath at 90°C for 10min, and finally centrifuging (11000r / min, 15min) to obtain Antarctic krill enzymatic hydrolyzate; The volume ratio of krill to distilled water is 1:3;

[0023] (2) Grind quicklime, pass through a 120-mesh standard sieve, dry at 90-105°C for 4 hours, and store in a desiccator for later use; the amount of quicklime added is 10g / L enzymatic hydrolysis solution, and stirred for 50min at pH 11.5; Finally, remove the reactants, use a filter membrane with a diameter of 0.40 μm to filter out the reactants and the remaining quicklime after the reaction, and the filtrate is the Antarctic krill h...

Embodiment 2

[0025] (1) Antarctic krill was mashed, then added distilled water to homogenate to obtain Antarctic krill shrimp paste, and then added Alcalase protease (measured enzyme activity 109387U / g, purchased from Novozyme (China) Biotechnology Co., Ltd.), enzyme The amount is 3000U / g, under the conditions of 53°C and pH value of 8.2, enzymolyze for 220min, finally inactivate the enzyme, and centrifuge to obtain Antarctic krill enzymatic hydrolyzate; wherein the volume ratio of Antarctic krill to distilled water is 1:4;

[0026] (2) Add quicklime to the enzymatic hydrolyzate at a concentration of 8g / L, and stir for 45min at a pH value of 11.0; finally filter, and the filtrate is an Antarctic krill hydrolyzate with reduced fluorine content.

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Abstract

The invention relates to a method for reducing the content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime, comprising the following steps of: (1) mashing the Euphausia superb, then adding distilled water for homogenating to obtain Euphausia superb pulp, and then adding Alcalase protease, carrying out enzymatic hydrolysis under the conditions with a temperature of 50-55 DEG C and a pH value of 8.0-8.5 for 220-260min, and finally killing enzyme and centrifuging to obtain enzymatic hydrolysate of the Euphausia superb; and (2) adding calcined lime with the concentration of 8-12g/L into the enzymatic hydrolysate, and stirring under the condition with the pH value of 10.0-11.5 for 40-50min; and finally filtering to obtain filtrate, i.e., the enzymatic hydrolysate of the Euphausia superb, which has reduced content of fluorine. In the invention, the calcined lime as the raw material is low in price and wide in source; and the method has a good fluorine reduction effect without lowering the nutritional value of the enzymatic hydrolysate of the Euphausia superb, and can be used for completely removing products and remained calcined lime simply after fluorinereduction, thereby having a wide application prospect.

Description

technical field [0001] The invention belongs to the field of methods for reducing fluorine content in solutions, in particular to a method for reducing fluorine content in Antarctic krill enzymatic hydrolyzate by using quicklime. Background technique [0002] Antarctic krill is very rich in resources, rich in nutrients, high in protein, fat, mineral elements, etc. Therefore, as a new food resource for human beings, it has great potential for development and utilization. Antarctic krill contains a large amount of fluorine. German scientist Soevik et al. measured that the fluorine content of frozen krill is about 2400mg / kg. An appropriate amount of fluorine is beneficial to the growth of human bones and teeth, but excessive intake of fluorine can lead to bone deformities. The World Health Organization (WHO) stipulates that the maximum fluorine intake limit for adults is not higher than 4mg / d. This shows that the fluorine in Antarctic krill is far beyond the adaptability of th...

Claims

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Application Information

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IPC IPC(8): A23L1/327A23L1/015A23L17/20A23L5/20
Inventor 李学英杨宪时吕传萍郭全友
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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