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Mutant strain ZN0871v11 and purpose thereof in rapid and efficient decolouring treatment of printing and dyeing waste water

A zn0871v11 printing and dyeing wastewater technology, which is applied in textile industry wastewater treatment, biological water/sewage treatment, water/sludge/sewage treatment, etc., can solve the pollution of rivers and lakes in the treatment of printing and dyeing wastewater, long running time, and increased difficulty in wastewater treatment And other issues

Active Publication Date: 2012-02-15
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of organic pollutants in printing and dyeing wastewater increases, the chroma becomes darker, the alkalinity becomes larger, the composition becomes more complex, and the difficulty of wastewater treatment increases
In addition, with the application of new dyes, changes in printing and dyeing process and product structure, the composition of printing and dyeing wastewater has also changed, which further increases the difficulty of wastewater treatment
Improper treatment of printing and dyeing wastewater will cause water pollution in rivers and lakes and damage to the ecological environment.
However, the currently used wastewater treatment process takes a long time to run, costs are high, and the effluent is often difficult to meet the standard, especially when the water volume is large.

Method used

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  • Mutant strain ZN0871v11 and purpose thereof in rapid and efficient decolouring treatment of printing and dyeing waste water
  • Mutant strain ZN0871v11 and purpose thereof in rapid and efficient decolouring treatment of printing and dyeing waste water
  • Mutant strain ZN0871v11 and purpose thereof in rapid and efficient decolouring treatment of printing and dyeing waste water

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1 Operation steps of the present invention

[0022] In the first step, the strain is activated. The starting strain ZN0871 isolated from Nanhu water samples was inoculated into liquid LB medium (peptone 10g / L, yeast extract 5g / L, NaCl 5g / L, pH 7.0) at an inoculum size of 1% and cultured for 10h for activation , and then transferred to fresh liquid LB culture medium for 10h.

[0023] The second step, mutagenesis. Turn on the ultraviolet lamp (15W) to preheat for 20 minutes, add 3mL of the activated bacterial solution into a small petri dish with a diameter of 6cm (put a paperclip in the dish before sterilization, the bottom is a magnetic stirrer), put the petri dish under the ultraviolet light Under the lamp, with a distance of 18cm, cover the entire purification workbench with a shading cloth, then open the lid and irradiate for 15s, 30s, 45s, 1min, 1min 15s, 1min 30s under stirring conditions, cover the lid after irradiation, and then turn off the UV lamp...

Embodiment 2

[0029] Example 2 Rapid and efficient decolorization treatment of printing and dyeing wastewater under various conditions

[0030] In this example, according to the method described in Example 1 above, the rapid and efficient decolorization effect of mutant ZN0871v11 cells on different dyes was verified under various conditions such as pH, metal ions, and temperature. It should be understood that the various conditions described below are exemplary only, and should not be construed as limiting the scope of the present invention.

Embodiment 2-1

[0032] At pH 5.0, temperature 37°C, metal ion Fe 2+ At a final concentration of 20mg / 100mL, the mutant ZN0871v11 cells were added and mixed within 10 minutes. The decolorization rate of the mutant ZN0871v11 to direct blue was 100%, and the COD caused by the dye was measured to be reduced to "0".

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Abstract

The invention discloses a mutagenicity screening method for a mutant strain ZN0871v11 for rapid and efficient decolouring treatment of printing and dyeing waste water and a novel technical method of the mutant strain ZN0871v11 for rapid and efficient decolouring treatment of printing and dyeing waste water. After genetic mutation of original bacterial strains, the mutant strain ZN0871v11 is screened out; the mutant strain ZN0871v11 has rapid, efficient and excellent decolouring effect on dyes such as benzo fast blue; and a decolouring rate is raised by 80% to 100%.

Description

technical field [0001] The invention belongs to the field of printing and dyeing wastewater treatment, and in particular relates to a new technology for rapid and efficient treatment of printing and dyeing wastewater by mutant strains. Background technique [0002] The textile industry is an important part of our country's economy and plays a pivotal role in the development of the national economy and the improvement of people's living standards. However, while the textile industry increases GDP, it also produces a large amount of printing and dyeing wastewater that is difficult to treat. The printing and dyeing industry is a high water consumption and high pollution industry. The "First National Pollution Source Survey Bulletin" shows that the annual water consumption of my country's printing and dyeing industry in 2007 exceeded 10 billion tons, which is about three times that of developed countries, of which 80% to 90% It is discharged as printing and dyeing wastewater. A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F1/52C02F3/34C12R1/125
CPCC02F3/34C02F2101/308C02F2103/30C12N1/205C12R2001/125
Inventor 祝伟曹霞察冬梅陈胜慧李步海孙小梅
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES